Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00275 and MMP_RS00280 are separated by 50 nucleotides MMP_RS00280 and MMP_RS00285 are separated by 4 nucleotides MMP_RS00285 and MMP_RS00290 are separated by 57 nucleotides MMP_RS00290 and MMP_RS00295 are separated by 38 nucleotides MMP_RS00295 and MMP_RS00300 are separated by 48 nucleotides
MMP_RS00275: MMP_RS00275 - polyprenyl synthetase family protein, at 62,928 to 63,881
_RS00275
MMP_RS00280: MMP_RS00280 - DUF2118 domain-containing protein, at 63,932 to 64,384
_RS00280
MMP_RS00285: MMP_RS00285 - TIGR04013 family B12-binding domain/radical SAM domain-containing protein, at 64,389 to 65,498
_RS00285
MMP_RS00290: MMP_RS00290 - hypothetical protein, at 65,556 to 65,828
_RS00290
MMP_RS00295: MMP_RS00295 - gamma carbonic anhydrase family protein, at 65,867 to 66,322
_RS00295
MMP_RS00300: MMP_RS00300 - 6 7-dimethyl-8-ribityllumazine synthase, at 66,371 to 66,784
_RS00300
Position (kb)
64
65
66 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 63.759 kb on - strand at 63.804 kb on - strand at 63.889 kb on - strand at 63.897 kb on - strand at 63.898 kb on + strand at 63.919 kb on - strand at 64.061 kb on - strand, within MMP_RS00280 at 64.071 kb on - strand, within MMP_RS00280 at 64.091 kb on - strand, within MMP_RS00280 at 64.128 kb on - strand, within MMP_RS00280 at 64.147 kb on - strand, within MMP_RS00280 at 64.154 kb on + strand, within MMP_RS00280 at 64.154 kb on + strand, within MMP_RS00280 at 64.162 kb on - strand, within MMP_RS00280 at 64.181 kb on + strand, within MMP_RS00280 at 64.203 kb on + strand, within MMP_RS00280 at 64.287 kb on - strand, within MMP_RS00280 at 64.292 kb on - strand, within MMP_RS00280 at 64.293 kb on + strand, within MMP_RS00280 at 64.293 kb on + strand, within MMP_RS00280 at 64.297 kb on - strand, within MMP_RS00280 at 64.309 kb on + strand, within MMP_RS00280 at 64.323 kb on - strand, within MMP_RS00280 at 64.328 kb on + strand, within MMP_RS00280 at 64.358 kb on - strand at 64.358 kb on - strand at 64.359 kb on + strand at 64.362 kb on - strand at 64.464 kb on + strand at 64.534 kb on - strand, within MMP_RS00285 at 64.535 kb on + strand, within MMP_RS00285 at 64.570 kb on - strand, within MMP_RS00285 at 64.632 kb on + strand, within MMP_RS00285 at 64.722 kb on - strand, within MMP_RS00285 at 64.766 kb on - strand, within MMP_RS00285 at 64.766 kb on - strand, within MMP_RS00285 at 64.777 kb on - strand, within MMP_RS00285 at 64.788 kb on - strand, within MMP_RS00285 at 64.824 kb on - strand, within MMP_RS00285 at 65.009 kb on - strand, within MMP_RS00285 at 65.009 kb on - strand, within MMP_RS00285 at 65.293 kb on + strand, within MMP_RS00285 at 65.371 kb on + strand, within MMP_RS00285 at 65.373 kb on - strand, within MMP_RS00285 at 65.437 kb on + strand at 65.464 kb on - strand at 65.464 kb on - strand at 65.465 kb on + strand at 65.498 kb on - strand at 65.499 kb on + strand at 65.508 kb on - strand at 65.521 kb on - strand at 65.522 kb on + strand at 65.522 kb on + strand at 65.522 kb on + strand at 65.523 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.577 kb on - strand at 65.577 kb on - strand at 65.578 kb on + strand at 65.578 kb on + strand at 65.578 kb on + strand at 65.587 kb on - strand, within MMP_RS00290 at 65.587 kb on - strand, within MMP_RS00290 at 65.619 kb on - strand, within MMP_RS00290 at 65.625 kb on - strand, within MMP_RS00290 at 65.664 kb on - strand, within MMP_RS00290 at 65.755 kb on - strand, within MMP_RS00290 at 65.773 kb on - strand, within MMP_RS00290 at 65.854 kb on - strand at 65.855 kb on + strand at 65.864 kb on + strand at 65.865 kb on - strand at 65.866 kb on + strand at 65.946 kb on - strand, within MMP_RS00295 at 66.252 kb on - strand, within MMP_RS00295 at 66.320 kb on - strand at 66.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 63,759 - -1.4 63,804 - +1.7 63,889 - +0.1 63,897 - -0.1 63,898 + -0.1 63,919 - -0.1 64,061 - MMP_RS00280 0.28 +0.2 64,071 - MMP_RS00280 0.31 -0.2 64,091 - MMP_RS00280 0.35 +0.1 64,128 - MMP_RS00280 0.43 +0.2 64,147 - MMP_RS00280 0.47 +0.1 64,154 + MMP_RS00280 0.49 +0.2 64,154 + MMP_RS00280 0.49 -0.1 64,162 - MMP_RS00280 0.51 -0.0 64,181 + MMP_RS00280 0.55 -0.3 64,203 + MMP_RS00280 0.60 +0.1 64,287 - MMP_RS00280 0.78 -0.1 64,292 - MMP_RS00280 0.79 -0.5 64,293 + MMP_RS00280 0.80 -0.2 64,293 + MMP_RS00280 0.80 +0.7 64,297 - MMP_RS00280 0.81 +0.3 64,309 + MMP_RS00280 0.83 +0.2 64,323 - MMP_RS00280 0.86 +0.2 64,328 + MMP_RS00280 0.87 +0.2 64,358 - -0.1 64,358 - -0.1 64,359 + -0.1 64,362 - -0.1 64,464 + +0.2 64,534 - MMP_RS00285 0.13 +0.2 64,535 + MMP_RS00285 0.13 +0.3 64,570 - MMP_RS00285 0.16 -0.2 64,632 + MMP_RS00285 0.22 -0.3 64,722 - MMP_RS00285 0.30 -0.6 64,766 - MMP_RS00285 0.34 +0.2 64,766 - MMP_RS00285 0.34 -0.0 64,777 - MMP_RS00285 0.35 +0.7 64,788 - MMP_RS00285 0.36 +1.8 64,824 - MMP_RS00285 0.39 +0.2 65,009 - MMP_RS00285 0.56 -0.1 65,009 - MMP_RS00285 0.56 -0.3 65,293 + MMP_RS00285 0.81 +0.3 65,371 + MMP_RS00285 0.88 -0.4 65,373 - MMP_RS00285 0.89 +0.5 65,437 + +0.5 65,464 - -0.1 65,464 - +0.2 65,465 + -0.2 65,498 - +0.2 65,499 + -0.1 65,508 - -1.4 65,521 - -0.9 65,522 + -0.1 65,522 + -0.0 65,522 + -0.4 65,523 - -0.4 65,575 - -0.9 65,575 - -1.0 65,575 - -0.5 65,575 - -0.5 65,575 - -1.8 65,577 - -0.5 65,577 - -0.9 65,578 + -0.7 65,578 + -0.7 65,578 + -0.4 65,587 - MMP_RS00290 0.11 -1.5 65,587 - MMP_RS00290 0.11 -1.2 65,619 - MMP_RS00290 0.23 -0.6 65,625 - MMP_RS00290 0.25 -0.4 65,664 - MMP_RS00290 0.40 -2.0 65,755 - MMP_RS00290 0.73 -1.2 65,773 - MMP_RS00290 0.79 -0.2 65,854 - -2.1 65,855 + -1.9 65,864 + -1.9 65,865 - -1.9 65,866 + -1.3 65,946 - MMP_RS00295 0.17 -1.5 66,252 - MMP_RS00295 0.84 -1.5 66,320 - -1.5 66,320 - -4.4
Or see this region's nucleotide sequence