Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19840 and IAI47_19845 are separated by 29 nucleotides IAI47_19845 and IAI47_19850 are separated by 164 nucleotides IAI47_19850 and IAI47_19855 are separated by 313 nucleotides
IAI47_19840: IAI47_19840 - DUF3147 family protein, at 195,957 to 196,307
_19840
IAI47_19845: IAI47_19845 - VIT family protein, at 196,337 to 197,020
_19845
IAI47_19850: IAI47_19850 - hypothetical protein, at 197,185 to 197,364
_19850
IAI47_19855: IAI47_19855 - DUF1345 domain-containing protein, at 197,678 to 198,337
_19855
Position (kb) 197
198 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 196.191 kb on + strand, within IAI47_19840 at 196.191 kb on + strand, within IAI47_19840 at 196.197 kb on + strand, within IAI47_19840 at 196.197 kb on + strand, within IAI47_19840 at 196.198 kb on - strand, within IAI47_19840 at 196.198 kb on - strand, within IAI47_19840 at 196.198 kb on - strand, within IAI47_19840 at 196.225 kb on + strand, within IAI47_19840 at 196.226 kb on - strand, within IAI47_19840 at 196.232 kb on - strand, within IAI47_19840 at 196.241 kb on - strand, within IAI47_19840 at 196.293 kb on - strand at 196.293 kb on - strand at 196.301 kb on + strand at 196.301 kb on + strand at 196.301 kb on + strand at 196.302 kb on - strand at 196.302 kb on - strand at 196.302 kb on - strand at 196.305 kb on + strand at 196.305 kb on + strand at 196.305 kb on + strand at 196.305 kb on + strand at 196.305 kb on + strand at 196.305 kb on + strand at 196.306 kb on - strand at 196.306 kb on - strand at 196.306 kb on - strand at 196.306 kb on - strand at 196.306 kb on - strand at 196.306 kb on - strand at 196.306 kb on - strand at 196.309 kb on + strand at 196.309 kb on + strand at 196.310 kb on - strand at 196.310 kb on - strand at 196.310 kb on - strand at 196.310 kb on - strand at 196.310 kb on - strand at 196.310 kb on - strand at 196.310 kb on - strand at 196.322 kb on - strand at 196.322 kb on - strand at 196.428 kb on - strand, within IAI47_19845 at 196.458 kb on - strand, within IAI47_19845 at 196.495 kb on + strand, within IAI47_19845 at 196.496 kb on - strand, within IAI47_19845 at 196.496 kb on - strand, within IAI47_19845 at 196.501 kb on + strand, within IAI47_19845 at 196.519 kb on - strand, within IAI47_19845 at 196.519 kb on - strand, within IAI47_19845 at 196.519 kb on - strand, within IAI47_19845 at 196.534 kb on + strand, within IAI47_19845 at 196.534 kb on + strand, within IAI47_19845 at 196.544 kb on + strand, within IAI47_19845 at 196.545 kb on - strand, within IAI47_19845 at 196.609 kb on + strand, within IAI47_19845 at 196.610 kb on - strand, within IAI47_19845 at 196.610 kb on - strand, within IAI47_19845 at 196.610 kb on - strand, within IAI47_19845 at 196.610 kb on - strand, within IAI47_19845 at 196.636 kb on + strand, within IAI47_19845 at 196.637 kb on - strand, within IAI47_19845 at 196.637 kb on - strand, within IAI47_19845 at 196.696 kb on + strand, within IAI47_19845 at 196.697 kb on - strand, within IAI47_19845 at 196.715 kb on - strand, within IAI47_19845 at 196.715 kb on - strand, within IAI47_19845 at 196.715 kb on - strand, within IAI47_19845 at 196.716 kb on + strand, within IAI47_19845 at 196.716 kb on + strand, within IAI47_19845 at 196.716 kb on + strand, within IAI47_19845 at 196.716 kb on + strand, within IAI47_19845 at 196.716 kb on + strand, within IAI47_19845 at 196.717 kb on - strand, within IAI47_19845 at 196.717 kb on - strand, within IAI47_19845 at 196.717 kb on - strand, within IAI47_19845 at 196.717 kb on - strand, within IAI47_19845 at 196.717 kb on - strand, within IAI47_19845 at 196.717 kb on - strand, within IAI47_19845 at 196.717 kb on - strand, within IAI47_19845 at 196.749 kb on + strand, within IAI47_19845 at 196.749 kb on + strand, within IAI47_19845 at 196.749 kb on + strand, within IAI47_19845 at 196.750 kb on - strand, within IAI47_19845 at 196.824 kb on + strand, within IAI47_19845 at 196.825 kb on - strand, within IAI47_19845 at 196.869 kb on - strand, within IAI47_19845 at 196.888 kb on + strand, within IAI47_19845 at 196.889 kb on - strand, within IAI47_19845 at 196.891 kb on + strand, within IAI47_19845 at 196.891 kb on + strand, within IAI47_19845 at 196.892 kb on - strand, within IAI47_19845 at 196.892 kb on - strand, within IAI47_19845 at 197.015 kb on + strand at 197.015 kb on + strand at 197.015 kb on + strand at 197.015 kb on + strand at 197.016 kb on - strand at 197.016 kb on - strand at 197.016 kb on - strand at 197.027 kb on - strand at 197.056 kb on - strand at 197.113 kb on - strand at 197.131 kb on - strand at 197.131 kb on - strand at 197.132 kb on + strand at 197.133 kb on - strand at 197.285 kb on + strand, within IAI47_19850 at 197.285 kb on + strand, within IAI47_19850 at 197.285 kb on + strand, within IAI47_19850 at 197.286 kb on - strand, within IAI47_19850 at 197.286 kb on - strand, within IAI47_19850 at 197.286 kb on - strand, within IAI47_19850 at 197.286 kb on - strand, within IAI47_19850 at 197.286 kb on - strand, within IAI47_19850 at 197.286 kb on - strand, within IAI47_19850 at 197.307 kb on + strand, within IAI47_19850 at 197.308 kb on - strand, within IAI47_19850 at 197.339 kb on + strand, within IAI47_19850 at 197.340 kb on - strand, within IAI47_19850 at 197.340 kb on - strand, within IAI47_19850 at 197.356 kb on + strand at 197.408 kb on + strand at 197.424 kb on + strand at 197.425 kb on - strand at 197.425 kb on - strand at 197.428 kb on + strand at 197.428 kb on + strand at 197.428 kb on + strand at 197.429 kb on - strand at 197.429 kb on - strand at 197.429 kb on - strand at 197.444 kb on + strand at 197.444 kb on + strand at 197.450 kb on + strand at 197.451 kb on - strand at 197.464 kb on + strand at 197.465 kb on - strand at 197.486 kb on + strand at 197.487 kb on - strand at 197.487 kb on - strand at 197.491 kb on + strand at 197.491 kb on + strand at 197.491 kb on + strand at 197.492 kb on - strand at 197.492 kb on - strand at 197.520 kb on - strand at 197.520 kb on - strand at 197.526 kb on + strand at 197.526 kb on + strand at 197.526 kb on + strand at 197.526 kb on + strand at 197.527 kb on - strand at 197.527 kb on - strand at 197.527 kb on - strand at 197.527 kb on - strand at 197.527 kb on - strand at 197.527 kb on - strand at 197.527 kb on - strand at 197.548 kb on - strand at 197.548 kb on - strand at 197.586 kb on + strand at 197.595 kb on + strand at 197.595 kb on + strand at 197.596 kb on - strand at 197.599 kb on + strand at 197.599 kb on + strand at 197.599 kb on + strand at 197.600 kb on - strand at 197.600 kb on - strand at 197.610 kb on + strand at 197.610 kb on + strand at 197.611 kb on - strand at 197.611 kb on - strand at 197.637 kb on + strand at 197.637 kb on + strand at 197.638 kb on - strand at 197.647 kb on - strand at 197.665 kb on + strand at 197.666 kb on - strand at 197.674 kb on + strand at 197.675 kb on - strand at 197.675 kb on - strand at 197.675 kb on - strand at 197.675 kb on - strand at 197.675 kb on - strand at 197.691 kb on + strand at 197.692 kb on - strand at 197.705 kb on + strand at 197.705 kb on + strand at 197.705 kb on + strand at 197.705 kb on + strand at 197.705 kb on + strand at 197.705 kb on + strand at 197.705 kb on + strand at 197.705 kb on + strand at 197.706 kb on - strand at 197.706 kb on - strand at 197.706 kb on - strand at 197.706 kb on - strand at 197.745 kb on + strand, within IAI47_19855 at 197.745 kb on + strand, within IAI47_19855 at 197.745 kb on + strand, within IAI47_19855 at 197.760 kb on + strand, within IAI47_19855 at 197.760 kb on + strand, within IAI47_19855 at 197.801 kb on - strand, within IAI47_19855 at 197.825 kb on + strand, within IAI47_19855 at 197.825 kb on + strand, within IAI47_19855 at 197.825 kb on + strand, within IAI47_19855 at 197.875 kb on + strand, within IAI47_19855 at 197.875 kb on + strand, within IAI47_19855 at 197.875 kb on + strand, within IAI47_19855 at 197.876 kb on - strand, within IAI47_19855 at 197.876 kb on - strand, within IAI47_19855 at 197.881 kb on + strand, within IAI47_19855 at 197.881 kb on + strand, within IAI47_19855 at 197.881 kb on + strand, within IAI47_19855 at 197.881 kb on + strand, within IAI47_19855 at 197.881 kb on + strand, within IAI47_19855 at 197.881 kb on + strand, within IAI47_19855 at 197.881 kb on + strand, within IAI47_19855 at 197.882 kb on - strand, within IAI47_19855 at 197.882 kb on - strand, within IAI47_19855 at 197.941 kb on + strand, within IAI47_19855 at 197.941 kb on + strand, within IAI47_19855 at 197.942 kb on - strand, within IAI47_19855 at 197.942 kb on - strand, within IAI47_19855 at 197.950 kb on + strand, within IAI47_19855 at 197.951 kb on - strand, within IAI47_19855 at 197.951 kb on - strand, within IAI47_19855 at 198.083 kb on + strand, within IAI47_19855 at 198.083 kb on + strand, within IAI47_19855 at 198.083 kb on + strand, within IAI47_19855 at 198.083 kb on + strand, within IAI47_19855 at 198.084 kb on - strand, within IAI47_19855 at 198.084 kb on - strand, within IAI47_19855 at 198.084 kb on - strand, within IAI47_19855 at 198.084 kb on - strand, within IAI47_19855 at 198.084 kb on - strand, within IAI47_19855 at 198.084 kb on - strand, within IAI47_19855 at 198.084 kb on - strand, within IAI47_19855 at 198.084 kb on - strand, within IAI47_19855 at 198.098 kb on + strand, within IAI47_19855 at 198.098 kb on + strand, within IAI47_19855 at 198.103 kb on + strand, within IAI47_19855 at 198.132 kb on + strand, within IAI47_19855 at 198.176 kb on + strand, within IAI47_19855 at 198.176 kb on + strand, within IAI47_19855 at 198.245 kb on - strand, within IAI47_19855 at 198.256 kb on - strand, within IAI47_19855 at 198.268 kb on + strand, within IAI47_19855 at 198.268 kb on + strand, within IAI47_19855 at 198.293 kb on - strand at 198.293 kb on - strand at 198.327 kb on + strand at 198.328 kb on - strand at 198.335 kb on + strand at 198.335 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 196,191 + IAI47_19840 0.67 -0.4 196,191 + IAI47_19840 0.67 +1.5 196,197 + IAI47_19840 0.68 -0.4 196,197 + IAI47_19840 0.68 -0.9 196,198 - IAI47_19840 0.69 -1.6 196,198 - IAI47_19840 0.69 +4.1 196,198 - IAI47_19840 0.69 +0.9 196,225 + IAI47_19840 0.76 -1.0 196,226 - IAI47_19840 0.77 +3.8 196,232 - IAI47_19840 0.78 +0.9 196,241 - IAI47_19840 0.81 +1.0 196,293 - +1.2 196,293 - -1.6 196,301 + -0.4 196,301 + +1.0 196,301 + -0.5 196,302 - +0.2 196,302 - +0.1 196,302 - +1.4 196,305 + +0.1 196,305 + +1.8 196,305 + -1.4 196,305 + -0.8 196,305 + +1.7 196,305 + +3.3 196,306 - -1.4 196,306 - -0.6 196,306 - +0.5 196,306 - +0.1 196,306 - -2.5 196,306 - +2.3 196,306 - +0.0 196,309 + -1.8 196,309 + -0.5 196,310 - -0.7 196,310 - -0.4 196,310 - -0.9 196,310 - +1.5 196,310 - -2.6 196,310 - -1.2 196,310 - -0.2 196,322 - -0.5 196,322 - -2.1 196,428 - IAI47_19845 0.13 -0.4 196,458 - IAI47_19845 0.18 +0.6 196,495 + IAI47_19845 0.23 -2.0 196,496 - IAI47_19845 0.23 +1.1 196,496 - IAI47_19845 0.23 -0.0 196,501 + IAI47_19845 0.24 +0.9 196,519 - IAI47_19845 0.27 +3.2 196,519 - IAI47_19845 0.27 -1.1 196,519 - IAI47_19845 0.27 +1.7 196,534 + IAI47_19845 0.29 -1.5 196,534 + IAI47_19845 0.29 -0.3 196,544 + IAI47_19845 0.30 +0.7 196,545 - IAI47_19845 0.30 +0.2 196,609 + IAI47_19845 0.40 -1.1 196,610 - IAI47_19845 0.40 +0.3 196,610 - IAI47_19845 0.40 +1.2 196,610 - IAI47_19845 0.40 -0.9 196,610 - IAI47_19845 0.40 -2.4 196,636 + IAI47_19845 0.44 -2.0 196,637 - IAI47_19845 0.44 +0.4 196,637 - IAI47_19845 0.44 +1.2 196,696 + IAI47_19845 0.52 +0.2 196,697 - IAI47_19845 0.53 +1.2 196,715 - IAI47_19845 0.55 +2.9 196,715 - IAI47_19845 0.55 -0.5 196,715 - IAI47_19845 0.55 +2.0 196,716 + IAI47_19845 0.55 +1.2 196,716 + IAI47_19845 0.55 +0.7 196,716 + IAI47_19845 0.55 -0.6 196,716 + IAI47_19845 0.55 -0.8 196,716 + IAI47_19845 0.55 -0.4 196,717 - IAI47_19845 0.56 -0.6 196,717 - IAI47_19845 0.56 -2.0 196,717 - IAI47_19845 0.56 +0.8 196,717 - IAI47_19845 0.56 +0.5 196,717 - IAI47_19845 0.56 +0.9 196,717 - IAI47_19845 0.56 +1.6 196,717 - IAI47_19845 0.56 -1.9 196,749 + IAI47_19845 0.60 -0.8 196,749 + IAI47_19845 0.60 -1.8 196,749 + IAI47_19845 0.60 +0.5 196,750 - IAI47_19845 0.60 +2.2 196,824 + IAI47_19845 0.71 -0.1 196,825 - IAI47_19845 0.71 +0.9 196,869 - IAI47_19845 0.78 +1.0 196,888 + IAI47_19845 0.81 -0.1 196,889 - IAI47_19845 0.81 +2.8 196,891 + IAI47_19845 0.81 +1.5 196,891 + IAI47_19845 0.81 +0.8 196,892 - IAI47_19845 0.81 +1.6 196,892 - IAI47_19845 0.81 +0.7 197,015 + -1.4 197,015 + +3.5 197,015 + +0.8 197,015 + +1.2 197,016 - -1.5 197,016 - +1.7 197,016 - +0.1 197,027 - +1.2 197,056 - -0.2 197,113 - -0.8 197,131 - -0.8 197,131 - +0.7 197,132 + +0.8 197,133 - -0.0 197,285 + IAI47_19850 0.56 -1.1 197,285 + IAI47_19850 0.56 -0.6 197,285 + IAI47_19850 0.56 +1.0 197,286 - IAI47_19850 0.56 +2.5 197,286 - IAI47_19850 0.56 -0.2 197,286 - IAI47_19850 0.56 +1.1 197,286 - IAI47_19850 0.56 +1.2 197,286 - IAI47_19850 0.56 -1.4 197,286 - IAI47_19850 0.56 +0.7 197,307 + IAI47_19850 0.68 +0.0 197,308 - IAI47_19850 0.68 +2.2 197,339 + IAI47_19850 0.86 -2.0 197,340 - IAI47_19850 0.86 -0.8 197,340 - IAI47_19850 0.86 +0.3 197,356 + +0.6 197,408 + -1.2 197,424 + +2.2 197,425 - -0.1 197,425 - -2.3 197,428 + +0.2 197,428 + -0.5 197,428 + -0.2 197,429 - -0.1 197,429 - +0.3 197,429 - -1.0 197,444 + -0.4 197,444 + -1.4 197,450 + -1.3 197,451 - +0.1 197,464 + +0.2 197,465 - +0.9 197,486 + +2.0 197,487 - +0.2 197,487 - +1.5 197,491 + +1.2 197,491 + -0.2 197,491 + -1.5 197,492 - +1.0 197,492 - +4.0 197,520 - +0.9 197,520 - -0.0 197,526 + +0.5 197,526 + -1.8 197,526 + -0.7 197,526 + -2.7 197,527 - -0.5 197,527 - +1.7 197,527 - -1.6 197,527 - -0.6 197,527 - +0.2 197,527 - +0.5 197,527 - -1.6 197,548 - -1.0 197,548 - -1.4 197,586 + -0.8 197,595 + -1.6 197,595 + +1.6 197,596 - -1.6 197,599 + -0.1 197,599 + -1.4 197,599 + -0.7 197,600 - -0.5 197,600 - -0.7 197,610 + -0.9 197,610 + -1.0 197,611 - +3.1 197,611 - -1.1 197,637 + -1.6 197,637 + -0.1 197,638 - -0.4 197,647 - -0.6 197,665 + -2.5 197,666 - -0.8 197,674 + +0.2 197,675 - -0.1 197,675 - -2.5 197,675 - +0.4 197,675 - -0.3 197,675 - +1.3 197,691 + -0.5 197,692 - -2.9 197,705 + +1.2 197,705 + +1.4 197,705 + +1.8 197,705 + -0.8 197,705 + -1.4 197,705 + +0.8 197,705 + -0.8 197,705 + +0.6 197,706 - -0.4 197,706 - -0.1 197,706 - +0.9 197,706 - -0.1 197,745 + IAI47_19855 0.10 +0.2 197,745 + IAI47_19855 0.10 -2.3 197,745 + IAI47_19855 0.10 -0.5 197,760 + IAI47_19855 0.12 -0.2 197,760 + IAI47_19855 0.12 +3.0 197,801 - IAI47_19855 0.19 -2.1 197,825 + IAI47_19855 0.22 -1.2 197,825 + IAI47_19855 0.22 -1.6 197,825 + IAI47_19855 0.22 -1.2 197,875 + IAI47_19855 0.30 -1.6 197,875 + IAI47_19855 0.30 -0.2 197,875 + IAI47_19855 0.30 -0.7 197,876 - IAI47_19855 0.30 +0.3 197,876 - IAI47_19855 0.30 +0.7 197,881 + IAI47_19855 0.31 +1.2 197,881 + IAI47_19855 0.31 -0.6 197,881 + IAI47_19855 0.31 +0.6 197,881 + IAI47_19855 0.31 -1.9 197,881 + IAI47_19855 0.31 -1.4 197,881 + IAI47_19855 0.31 +1.9 197,881 + IAI47_19855 0.31 -0.0 197,882 - IAI47_19855 0.31 -3.3 197,882 - IAI47_19855 0.31 +1.0 197,941 + IAI47_19855 0.40 -1.5 197,941 + IAI47_19855 0.40 -0.3 197,942 - IAI47_19855 0.40 +0.2 197,942 - IAI47_19855 0.40 +1.5 197,950 + IAI47_19855 0.41 -0.0 197,951 - IAI47_19855 0.41 +1.2 197,951 - IAI47_19855 0.41 +1.2 198,083 + IAI47_19855 0.61 -1.1 198,083 + IAI47_19855 0.61 -1.3 198,083 + IAI47_19855 0.61 -0.8 198,083 + IAI47_19855 0.61 +0.6 198,084 - IAI47_19855 0.62 +2.4 198,084 - IAI47_19855 0.62 +1.6 198,084 - IAI47_19855 0.62 +0.6 198,084 - IAI47_19855 0.62 -2.1 198,084 - IAI47_19855 0.62 -0.0 198,084 - IAI47_19855 0.62 -3.8 198,084 - IAI47_19855 0.62 -0.4 198,084 - IAI47_19855 0.62 +1.6 198,098 + IAI47_19855 0.64 +2.2 198,098 + IAI47_19855 0.64 -2.0 198,103 + IAI47_19855 0.64 -0.5 198,132 + IAI47_19855 0.69 -2.7 198,176 + IAI47_19855 0.75 +0.4 198,176 + IAI47_19855 0.75 -0.8 198,245 - IAI47_19855 0.86 +1.2 198,256 - IAI47_19855 0.88 +1.2 198,268 + IAI47_19855 0.89 +1.1 198,268 + IAI47_19855 0.89 -1.1 198,293 - -0.4 198,293 - +3.5 198,327 + +1.4 198,328 - +1.3 198,335 + -0.6 198,335 + +1.9
Or see this region's nucleotide sequence