Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_16130 and IAI47_16135 overlap by 20 nucleotides IAI47_16135 and IAI47_16140 are separated by 3 nucleotides IAI47_16140 and IAI47_16145 overlap by 1 nucleotides
IAI47_16130: IAI47_16130 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 3,415,050 to 3,416,003
_16130
IAI47_16135: IAI47_16135 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 3,415,984 to 3,416,454
_16135
IAI47_16140: IAI47_16140 - signal peptidase II, at 3,416,458 to 3,416,964
_16140
IAI47_16145: IAI47_16145 - isoleucine--tRNA ligase, at 3,416,964 to 3,419,780
_16145
Position (kb)
3415
3416
3417 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3414.996 kb on + strand at 3414.997 kb on - strand at 3414.997 kb on - strand at 3414.997 kb on - strand at 3415.041 kb on - strand at 3415.041 kb on - strand at 3415.052 kb on - strand at 3415.052 kb on - strand at 3415.061 kb on + strand at 3415.061 kb on + strand at 3415.062 kb on - strand at 3415.062 kb on - strand at 3415.062 kb on - strand at 3415.062 kb on - strand at 3415.062 kb on - strand at 3415.062 kb on - strand at 3415.062 kb on - strand at 3415.065 kb on + strand at 3415.066 kb on - strand at 3415.066 kb on - strand at 3415.066 kb on - strand at 3415.066 kb on - strand at 3415.066 kb on - strand at 3415.085 kb on + strand at 3415.086 kb on - strand at 3415.086 kb on - strand at 3415.086 kb on - strand at 3416.042 kb on - strand, within IAI47_16135 at 3416.042 kb on - strand, within IAI47_16135 at 3416.042 kb on - strand, within IAI47_16135 at 3416.042 kb on - strand, within IAI47_16135 at 3416.042 kb on - strand, within IAI47_16135 at 3416.095 kb on - strand, within IAI47_16135 at 3416.095 kb on - strand, within IAI47_16135 at 3416.095 kb on - strand, within IAI47_16135 at 3416.095 kb on - strand, within IAI47_16135 at 3416.194 kb on - strand, within IAI47_16135 at 3416.194 kb on - strand, within IAI47_16135 at 3416.214 kb on - strand, within IAI47_16135 at 3416.266 kb on - strand, within IAI47_16135 at 3416.266 kb on - strand, within IAI47_16135 at 3416.410 kb on - strand at 3416.460 kb on - strand at 3416.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 3,414,996 + +1.2 3,414,997 - -0.5 3,414,997 - -0.9 3,414,997 - +1.4 3,415,041 - +1.4 3,415,041 - -0.4 3,415,052 - -0.8 3,415,052 - +0.6 3,415,061 + +0.7 3,415,061 + +1.2 3,415,062 - -0.1 3,415,062 - +1.0 3,415,062 - -0.8 3,415,062 - +0.8 3,415,062 - -0.3 3,415,062 - -0.1 3,415,062 - -0.7 3,415,065 + +0.4 3,415,066 - -0.6 3,415,066 - -2.1 3,415,066 - +0.4 3,415,066 - -0.6 3,415,066 - -0.2 3,415,085 + -0.3 3,415,086 - +1.6 3,415,086 - +3.2 3,415,086 - -1.8 3,416,042 - IAI47_16135 0.12 -0.2 3,416,042 - IAI47_16135 0.12 -1.0 3,416,042 - IAI47_16135 0.12 -0.3 3,416,042 - IAI47_16135 0.12 -2.1 3,416,042 - IAI47_16135 0.12 -0.1 3,416,095 - IAI47_16135 0.24 -0.7 3,416,095 - IAI47_16135 0.24 -0.1 3,416,095 - IAI47_16135 0.24 -1.5 3,416,095 - IAI47_16135 0.24 +1.2 3,416,194 - IAI47_16135 0.45 -0.7 3,416,194 - IAI47_16135 0.45 -1.8 3,416,214 - IAI47_16135 0.49 -1.5 3,416,266 - IAI47_16135 0.60 -0.5 3,416,266 - IAI47_16135 0.60 -0.9 3,416,410 - -0.3 3,416,460 - -1.8 3,416,460 - +1.1
Or see this region's nucleotide sequence