Experiment: ALP with Zinc 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1854 and Dsui_1855 are separated by 384 nucleotides Dsui_1855 and Dsui_1856 are separated by 102 nucleotides Dsui_1856 and Dsui_1857 overlap by 13 nucleotides
Dsui_1854: Dsui_1854 - hypothetical protein, at 1,948,314 to 1,949,579
_1854
Dsui_1855: Dsui_1855 - hypothetical protein, at 1,949,964 to 1,950,902
_1855
Dsui_1856: Dsui_1856 - 4-oxalocrotonate tautomerase family enzyme, at 1,951,005 to 1,951,214
_1856
Dsui_1857: Dsui_1857 - putative permease, DMT superfamily, at 1,951,202 to 1,952,101
_1857
Position (kb)
1949
1950
1951 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1949.126 kb on + strand at 1949.188 kb on - strand at 1949.212 kb on - strand, within Dsui_1854 at 1949.214 kb on - strand at 1949.240 kb on - strand at 1949.535 kb on + strand at 1949.608 kb on + strand at 1949.969 kb on - strand at 1950.089 kb on - strand, within Dsui_1855 at 1950.089 kb on - strand, within Dsui_1855 at 1950.099 kb on - strand at 1950.342 kb on + strand at 1950.521 kb on + strand at 1950.589 kb on + strand, within Dsui_1855 at 1950.590 kb on - strand at 1950.630 kb on - strand at 1950.637 kb on + strand, within Dsui_1855 at 1950.675 kb on - strand at 1950.739 kb on - strand at 1950.815 kb on - strand at 1951.000 kb on - strand at 1951.074 kb on - strand at 1951.182 kb on + strand, within Dsui_1856 at 1951.227 kb on - strand at 1951.368 kb on - strand, within Dsui_1857 at 1951.376 kb on - strand at 1951.385 kb on + strand at 1951.440 kb on - strand at 1951.536 kb on + strand at 1951.544 kb on - strand, within Dsui_1857 at 1951.572 kb on - strand, within Dsui_1857 at 1951.611 kb on + strand, within Dsui_1857 at 1951.845 kb on + strand at 1951.896 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Zinc 1 mM remove 1,949,126 + +0.6 1,949,188 - +0.6 1,949,212 - Dsui_1854 0.71 -0.4 1,949,214 - -0.4 1,949,240 - +0.6 1,949,535 + -1.4 1,949,608 + -0.4 1,949,969 - +0.6 1,950,089 - Dsui_1855 0.13 -2.7 1,950,089 - Dsui_1855 0.13 -1.0 1,950,099 - -1.0 1,950,342 + -0.4 1,950,521 + +0.6 1,950,589 + Dsui_1855 0.67 +0.6 1,950,590 - +0.6 1,950,630 - +0.6 1,950,637 + Dsui_1855 0.72 -2.8 1,950,675 - +0.6 1,950,739 - +0.6 1,950,815 - -1.4 1,951,000 - -2.6 1,951,074 - +0.6 1,951,182 + Dsui_1856 0.84 -1.7 1,951,227 - -1.7 1,951,368 - Dsui_1857 0.18 -2.2 1,951,376 - +0.6 1,951,385 + +0.6 1,951,440 - +0.6 1,951,536 + -1.0 1,951,544 - Dsui_1857 0.38 +0.6 1,951,572 - Dsui_1857 0.41 -0.4 1,951,611 + Dsui_1857 0.45 -0.4 1,951,845 + +0.6 1,951,896 - -0.4
Or see this region's nucleotide sequence