Experiment: ALP with Zinc 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1122 and Dsui_1123 are separated by 150 nucleotides Dsui_1123 and Dsui_1124 are separated by 49 nucleotides Dsui_1124 and Dsui_1125 are separated by 61 nucleotides
Dsui_1122: Dsui_1122 - dihydroxy-acid dehydratase, at 1,169,218 to 1,171,077
_1122
Dsui_1123: Dsui_1123 - methyl-accepting chemotaxis protein, at 1,171,228 to 1,172,841
_1123
Dsui_1124: Dsui_1124 - prolipoprotein diacylglyceryl transferase, at 1,172,891 to 1,173,676
_1124
Dsui_1125: Dsui_1125 - Malonyl-CoA decarboxylase (MCD), at 1,173,738 to 1,175,066
_1125
Position (kb)
1171
1172
1173 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1171.443 kb on - strand at 1171.458 kb on - strand, within Dsui_1123 at 1171.502 kb on + strand, within Dsui_1123 at 1171.573 kb on - strand, within Dsui_1123 at 1171.575 kb on + strand, within Dsui_1123 at 1171.575 kb on + strand, within Dsui_1123 at 1171.705 kb on - strand, within Dsui_1123 at 1171.897 kb on + strand, within Dsui_1123 at 1171.900 kb on - strand, within Dsui_1123 at 1172.097 kb on + strand, within Dsui_1123 at 1172.112 kb on - strand, within Dsui_1123 at 1172.198 kb on - strand, within Dsui_1123 at 1172.213 kb on + strand, within Dsui_1123 at 1172.242 kb on + strand, within Dsui_1123 at 1172.273 kb on + strand, within Dsui_1123 at 1172.490 kb on + strand, within Dsui_1123 at 1172.493 kb on - strand, within Dsui_1123 at 1172.515 kb on + strand, within Dsui_1123 at 1172.525 kb on + strand, within Dsui_1123 at 1172.532 kb on - strand, within Dsui_1123 at 1172.799 kb on + strand at 1172.799 kb on + strand at 1172.807 kb on - strand at 1172.863 kb on + strand at 1173.702 kb on - strand at 1173.807 kb on + strand at 1173.820 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Zinc 1 mM remove 1,171,443 - +0.3 1,171,458 - Dsui_1123 0.14 -0.7 1,171,502 + Dsui_1123 0.17 +0.3 1,171,573 - Dsui_1123 0.21 -2.3 1,171,575 + Dsui_1123 0.21 +1.9 1,171,575 + Dsui_1123 0.21 -1.5 1,171,705 - Dsui_1123 0.30 +0.3 1,171,897 + Dsui_1123 0.41 -2.3 1,171,900 - Dsui_1123 0.42 +0.3 1,172,097 + Dsui_1123 0.54 -0.1 1,172,112 - Dsui_1123 0.55 -1.7 1,172,198 - Dsui_1123 0.60 -1.7 1,172,213 + Dsui_1123 0.61 -0.7 1,172,242 + Dsui_1123 0.63 -0.1 1,172,273 + Dsui_1123 0.65 +0.1 1,172,490 + Dsui_1123 0.78 -1.0 1,172,493 - Dsui_1123 0.78 +0.3 1,172,515 + Dsui_1123 0.80 -0.7 1,172,525 + Dsui_1123 0.80 -0.3 1,172,532 - Dsui_1123 0.81 -0.7 1,172,799 + -2.1 1,172,799 + -0.8 1,172,807 - -0.7 1,172,863 + -0.4 1,173,702 - +0.3 1,173,807 + -0.2 1,173,820 + -0.1
Or see this region's nucleotide sequence