Experiment: ALP with Zinc 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0001 and Dsui_0002 are separated by 67 nucleotides Dsui_0002 and Dsui_0003 are separated by 80 nucleotides Dsui_0003 and Dsui_0004 are separated by 42 nucleotides Dsui_0004 and Dsui_0005 are separated by 32 nucleotides
Dsui_0001: Dsui_0001 - aminodeoxychorismate synthase, component I, at 103 to 1,923
_0001
Dsui_0002: Dsui_0002 - putative redox protein, regulator of disulfide bond formation, at 1,991 to 2,218
_0002
Dsui_0003: Dsui_0003 - putative Zn-dependent protease, at 2,299 to 3,786
_0003
Dsui_0004: Dsui_0004 - molybdenum cofactor biosynthesis protein MoaC, at 3,829 to 4,323
_0004
Dsui_0005: Dsui_0005 - putative secreted protein, at 4,356 to 4,826
_0005
Position (kb) 2
3
4 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1.360 kb on + strand, within Dsui_0001 at 1.368 kb on - strand, within Dsui_0001 at 1.488 kb on - strand, within Dsui_0001 at 1.514 kb on + strand, within Dsui_0001 at 1.613 kb on + strand, within Dsui_0001 at 1.621 kb on - strand, within Dsui_0001 at 1.810 kb on + strand at 1.823 kb on - strand at 1.846 kb on - strand at 1.846 kb on - strand at 1.894 kb on - strand at 2.403 kb on - strand at 2.450 kb on - strand, within Dsui_0003 at 2.534 kb on - strand, within Dsui_0003 at 2.548 kb on - strand, within Dsui_0003 at 2.572 kb on - strand, within Dsui_0003 at 2.577 kb on - strand, within Dsui_0003 at 2.977 kb on - strand, within Dsui_0003 at 3.026 kb on - strand, within Dsui_0003 at 3.131 kb on - strand at 3.222 kb on - strand, within Dsui_0003 at 3.413 kb on - strand, within Dsui_0003 at 3.503 kb on - strand, within Dsui_0003 at 3.503 kb on - strand at 3.503 kb on - strand, within Dsui_0003 at 3.567 kb on - strand, within Dsui_0003 at 3.791 kb on + strand at 3.833 kb on - strand at 3.833 kb on - strand at 3.861 kb on - strand at 4.008 kb on + strand, within Dsui_0004 at 4.035 kb on + strand, within Dsui_0004 at 4.079 kb on + strand, within Dsui_0004 at 4.093 kb on - strand at 4.099 kb on + strand, within Dsui_0004 at 4.205 kb on + strand, within Dsui_0004 at 4.230 kb on - strand, within Dsui_0004 at 4.241 kb on - strand at 4.260 kb on + strand, within Dsui_0004 at 4.426 kb on - strand, within Dsui_0005 at 4.426 kb on - strand at 4.504 kb on - strand, within Dsui_0005 at 4.522 kb on - strand, within Dsui_0005 at 4.630 kb on + strand, within Dsui_0005 at 4.631 kb on - strand, within Dsui_0005 at 4.695 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.713 kb on - strand, within Dsui_0005 at 4.725 kb on - strand, within Dsui_0005 at 4.742 kb on + strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.762 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Zinc 1 mM remove 1,360 + Dsui_0001 0.69 -1.2 1,368 - Dsui_0001 0.69 -0.3 1,488 - Dsui_0001 0.76 -0.5 1,514 + Dsui_0001 0.77 -2.2 1,613 + Dsui_0001 0.83 -1.9 1,621 - Dsui_0001 0.83 +0.1 1,810 + +0.4 1,823 - -0.3 1,846 - -0.3 1,846 - -0.3 1,894 - -1.2 2,403 - -3.2 2,450 - Dsui_0003 0.10 -2.4 2,534 - Dsui_0003 0.16 -3.9 2,548 - Dsui_0003 0.17 -0.8 2,572 - Dsui_0003 0.18 -5.3 2,577 - Dsui_0003 0.19 -3.7 2,977 - Dsui_0003 0.46 -3.3 3,026 - Dsui_0003 0.49 -1.8 3,131 - -1.9 3,222 - Dsui_0003 0.62 -2.3 3,413 - Dsui_0003 0.75 -0.6 3,503 - Dsui_0003 0.81 -2.2 3,503 - -1.9 3,503 - Dsui_0003 0.81 -2.8 3,567 - Dsui_0003 0.85 -3.5 3,791 + -0.4 3,833 - -1.2 3,833 - -1.2 3,861 - -1.6 4,008 + Dsui_0004 0.36 +0.1 4,035 + Dsui_0004 0.42 -0.5 4,079 + Dsui_0004 0.51 -0.2 4,093 - +0.4 4,099 + Dsui_0004 0.55 -0.1 4,205 + Dsui_0004 0.76 -0.3 4,230 - Dsui_0004 0.81 -1.2 4,241 - +0.4 4,260 + Dsui_0004 0.87 -0.3 4,426 - Dsui_0005 0.15 +0.4 4,426 - +0.4 4,504 - Dsui_0005 0.31 -0.6 4,522 - Dsui_0005 0.35 +0.4 4,630 + Dsui_0005 0.58 +0.0 4,631 - Dsui_0005 0.58 -2.2 4,695 + Dsui_0005 0.72 -0.5 4,705 + Dsui_0005 0.74 -0.4 4,705 + Dsui_0005 0.74 -0.2 4,705 + Dsui_0005 0.74 -0.5 4,705 + Dsui_0005 0.74 -0.1 4,713 - Dsui_0005 0.76 -0.6 4,725 - Dsui_0005 0.78 -0.6 4,742 + Dsui_0005 0.82 -0.3 4,750 - Dsui_0005 0.84 -1.2 4,750 - Dsui_0005 0.84 +0.4 4,750 - Dsui_0005 0.84 +0.4 4,750 - Dsui_0005 0.84 -1.6 4,762 - +0.4
Or see this region's nucleotide sequence