Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22680

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22670 and BWI76_RS22675 overlap by 1 nucleotidesBWI76_RS22675 and BWI76_RS22680 are separated by 19 nucleotidesBWI76_RS22680 and BWI76_RS22685 are separated by 139 nucleotidesBWI76_RS22685 and BWI76_RS22690 are separated by 53 nucleotides BWI76_RS22670: BWI76_RS22670 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 4,724,903 to 4,725,382 _RS22670 BWI76_RS22675: BWI76_RS22675 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 4,725,382 to 4,726,092 _RS22675 BWI76_RS22680: BWI76_RS22680 - cell division protein FtsB, at 4,726,112 to 4,726,429 _RS22680 BWI76_RS22685: BWI76_RS22685 - hypothetical protein, at 4,726,569 to 4,726,895 _RS22685 BWI76_RS22690: BWI76_RS22690 - adenylyl-sulfate kinase, at 4,726,949 to 4,727,554 _RS22690 Position (kb) 4726 4727Strain fitness (log2 ratio) -2 -1 0 1 2at 4726.585 kb on + strandat 4726.723 kb on + strand, within BWI76_RS22685at 4726.723 kb on + strand, within BWI76_RS22685at 4726.730 kb on - strand, within BWI76_RS22685at 4726.731 kb on - strand, within BWI76_RS22685at 4726.731 kb on - strand, within BWI76_RS22685at 4726.731 kb on - strand, within BWI76_RS22685at 4726.731 kb on - strand, within BWI76_RS22685at 4726.753 kb on + strand, within BWI76_RS22685at 4726.776 kb on - strand, within BWI76_RS22685at 4726.817 kb on + strand, within BWI76_RS22685at 4726.909 kb on + strandat 4726.964 kb on - strandat 4726.980 kb on - strandat 4726.980 kb on - strandat 4727.091 kb on + strand, within BWI76_RS22690at 4727.106 kb on + strand, within BWI76_RS22690at 4727.212 kb on + strand, within BWI76_RS22690at 4727.279 kb on - strand, within BWI76_RS22690at 4727.339 kb on + strand, within BWI76_RS22690

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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4,726,585 + -1.9
4,726,723 + BWI76_RS22685 0.47 +0.1
4,726,723 + BWI76_RS22685 0.47 +1.1
4,726,730 - BWI76_RS22685 0.49 -0.2
4,726,731 - BWI76_RS22685 0.50 -0.0
4,726,731 - BWI76_RS22685 0.50 +0.1
4,726,731 - BWI76_RS22685 0.50 -0.5
4,726,731 - BWI76_RS22685 0.50 +2.1
4,726,753 + BWI76_RS22685 0.56 +1.4
4,726,776 - BWI76_RS22685 0.63 +1.5
4,726,817 + BWI76_RS22685 0.76 +0.8
4,726,909 + -0.4
4,726,964 - +0.1
4,726,980 - -0.1
4,726,980 - +0.7
4,727,091 + BWI76_RS22690 0.23 -1.5
4,727,106 + BWI76_RS22690 0.26 -1.5
4,727,212 + BWI76_RS22690 0.43 +0.1
4,727,279 - BWI76_RS22690 0.54 +0.3
4,727,339 + BWI76_RS22690 0.64 -0.7

Or see this region's nucleotide sequence