Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13280

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13270 and BWI76_RS13275 are separated by 38 nucleotidesBWI76_RS13275 and BWI76_RS13280 are separated by 68 nucleotidesBWI76_RS13280 and BWI76_RS13285 are separated by 172 nucleotidesBWI76_RS13285 and BWI76_RS13290 overlap by 4 nucleotides BWI76_RS13270: BWI76_RS13270 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 2,766,240 to 2,768,612 _RS13270 BWI76_RS13275: BWI76_RS13275 - hypothetical protein, at 2,768,651 to 2,768,836 _RS13275 BWI76_RS13280: BWI76_RS13280 - LysR family transcriptional regulator, at 2,768,905 to 2,769,828 _RS13280 BWI76_RS13285: BWI76_RS13285 - protocatechuate 3,4-dioxygenase subunit alpha, at 2,770,001 to 2,770,621 _RS13285 BWI76_RS13290: BWI76_RS13290 - protocatechuate 3,4-dioxygenase subunit beta, at 2,770,618 to 2,771,358 _RS13290 Position (kb) 2768 2769 2770Strain fitness (log2 ratio) -1 0 1 2at 2767.984 kb on - strand, within BWI76_RS13270at 2768.034 kb on - strand, within BWI76_RS13270at 2768.044 kb on - strand, within BWI76_RS13270at 2768.068 kb on - strand, within BWI76_RS13270at 2768.281 kb on + strand, within BWI76_RS13270at 2768.573 kb on - strandat 2768.763 kb on + strand, within BWI76_RS13275at 2769.007 kb on - strand, within BWI76_RS13280at 2769.080 kb on - strand, within BWI76_RS13280at 2769.505 kb on - strand, within BWI76_RS13280at 2769.507 kb on - strand, within BWI76_RS13280at 2769.574 kb on - strand, within BWI76_RS13280at 2769.577 kb on - strand, within BWI76_RS13280at 2769.700 kb on + strand, within BWI76_RS13280at 2769.728 kb on - strand, within BWI76_RS13280at 2769.757 kb on - strandat 2769.783 kb on - strandat 2769.794 kb on + strandat 2770.020 kb on + strandat 2770.256 kb on + strand, within BWI76_RS13285at 2770.653 kb on + strandat 2770.661 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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2,767,984 - BWI76_RS13270 0.73 -0.3
2,768,034 - BWI76_RS13270 0.76 +0.5
2,768,044 - BWI76_RS13270 0.76 +0.1
2,768,068 - BWI76_RS13270 0.77 +0.3
2,768,281 + BWI76_RS13270 0.86 +0.4
2,768,573 - +0.6
2,768,763 + BWI76_RS13275 0.60 +0.8
2,769,007 - BWI76_RS13280 0.11 +1.9
2,769,080 - BWI76_RS13280 0.19 +1.1
2,769,505 - BWI76_RS13280 0.65 +1.0
2,769,507 - BWI76_RS13280 0.65 +1.2
2,769,574 - BWI76_RS13280 0.72 +1.3
2,769,577 - BWI76_RS13280 0.73 +1.3
2,769,700 + BWI76_RS13280 0.86 +1.1
2,769,728 - BWI76_RS13280 0.89 +1.0
2,769,757 - +1.9
2,769,783 - +1.4
2,769,794 + +1.0
2,770,020 + +2.6
2,770,256 + BWI76_RS13285 0.41 -0.0
2,770,653 + +0.5
2,770,661 - +0.4

Or see this region's nucleotide sequence