Experiment: Recovery broth with Trimethoprim 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12985 and BBR_RS12990 are separated by 87 nucleotides BBR_RS12990 and BBR_RS12995 are separated by 105 nucleotides
BBR_RS12985: BBR_RS12985 - MFS transporter, at 683,285 to 684,604
_RS12985
BBR_RS12990: BBR_RS12990 - 4-hydroxy-tetrahydrodipicolinate reductase, at 684,692 to 685,447
_RS12990
BBR_RS12995: BBR_RS12995 - 4-hydroxy-tetrahydrodipicolinate synthase, at 685,553 to 686,458
_RS12995
Position (kb) 684
685
686 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 683.714 kb on - strand, within BBR_RS12985 at 683.754 kb on - strand, within BBR_RS12985 at 683.771 kb on - strand, within BBR_RS12985 at 683.881 kb on + strand, within BBR_RS12985 at 683.917 kb on + strand, within BBR_RS12985 at 683.941 kb on - strand, within BBR_RS12985 at 683.965 kb on - strand, within BBR_RS12985 at 683.975 kb on - strand, within BBR_RS12985 at 684.010 kb on + strand, within BBR_RS12985 at 684.027 kb on - strand, within BBR_RS12985 at 684.039 kb on - strand, within BBR_RS12985 at 684.053 kb on + strand, within BBR_RS12985 at 684.059 kb on + strand, within BBR_RS12985 at 684.073 kb on - strand, within BBR_RS12985 at 684.074 kb on + strand, within BBR_RS12985 at 684.108 kb on + strand, within BBR_RS12985 at 684.116 kb on - strand, within BBR_RS12985 at 684.133 kb on + strand, within BBR_RS12985 at 684.147 kb on + strand, within BBR_RS12985 at 684.155 kb on - strand, within BBR_RS12985 at 684.165 kb on + strand, within BBR_RS12985 at 684.204 kb on + strand, within BBR_RS12985 at 684.283 kb on + strand, within BBR_RS12985 at 684.283 kb on + strand, within BBR_RS12985 at 684.323 kb on - strand, within BBR_RS12985 at 684.323 kb on - strand, within BBR_RS12985 at 684.341 kb on - strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.431 kb on - strand, within BBR_RS12985 at 684.433 kb on + strand, within BBR_RS12985 at 684.441 kb on - strand, within BBR_RS12985 at 684.468 kb on - strand, within BBR_RS12985 at 684.497 kb on - strand at 684.497 kb on - strand at 684.550 kb on + strand at 684.550 kb on + strand at 684.550 kb on + strand at 684.552 kb on + strand at 684.558 kb on - strand at 684.573 kb on - strand at 684.584 kb on - strand at 684.599 kb on + strand at 684.599 kb on + strand at 684.607 kb on - strand at 684.607 kb on - strand at 684.694 kb on + strand at 684.735 kb on + strand at 684.749 kb on + strand at 684.749 kb on + strand at 684.803 kb on + strand, within BBR_RS12990 at 684.808 kb on + strand, within BBR_RS12990 at 684.831 kb on - strand, within BBR_RS12990 at 684.856 kb on + strand, within BBR_RS12990 at 684.874 kb on - strand, within BBR_RS12990 at 684.879 kb on - strand, within BBR_RS12990 at 684.887 kb on - strand, within BBR_RS12990 at 684.908 kb on - strand, within BBR_RS12990 at 684.925 kb on + strand, within BBR_RS12990 at 684.926 kb on - strand, within BBR_RS12990 at 684.929 kb on - strand, within BBR_RS12990 at 684.952 kb on + strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.990 kb on + strand, within BBR_RS12990 at 684.997 kb on + strand, within BBR_RS12990 at 685.015 kb on - strand, within BBR_RS12990 at 685.077 kb on + strand, within BBR_RS12990 at 685.091 kb on + strand, within BBR_RS12990 at 685.096 kb on + strand, within BBR_RS12990 at 685.105 kb on + strand, within BBR_RS12990 at 685.113 kb on - strand, within BBR_RS12990 at 685.113 kb on - strand, within BBR_RS12990 at 685.123 kb on + strand, within BBR_RS12990 at 685.131 kb on - strand, within BBR_RS12990 at 685.139 kb on - strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.178 kb on - strand, within BBR_RS12990 at 685.229 kb on - strand, within BBR_RS12990 at 685.249 kb on + strand, within BBR_RS12990 at 685.257 kb on - strand, within BBR_RS12990 at 685.280 kb on - strand, within BBR_RS12990 at 685.280 kb on - strand, within BBR_RS12990 at 685.289 kb on - strand, within BBR_RS12990 at 685.297 kb on - strand, within BBR_RS12990 at 685.409 kb on + strand at 685.412 kb on - strand at 685.412 kb on - strand at 685.434 kb on + strand at 685.456 kb on - strand at 685.517 kb on + strand at 685.520 kb on + strand at 685.541 kb on + strand at 685.549 kb on + strand at 685.549 kb on + strand at 685.551 kb on + strand at 685.557 kb on - strand at 685.701 kb on + strand, within BBR_RS12995 at 685.701 kb on + strand, within BBR_RS12995 at 685.738 kb on + strand, within BBR_RS12995 at 685.764 kb on - strand, within BBR_RS12995 at 685.765 kb on + strand, within BBR_RS12995 at 685.784 kb on + strand, within BBR_RS12995 at 685.855 kb on + strand, within BBR_RS12995 at 685.859 kb on + strand, within BBR_RS12995 at 685.976 kb on + strand, within BBR_RS12995 at 686.008 kb on + strand, within BBR_RS12995 at 686.014 kb on + strand, within BBR_RS12995 at 686.014 kb on + strand, within BBR_RS12995 at 686.014 kb on + strand, within BBR_RS12995 at 686.014 kb on + strand, within BBR_RS12995 at 686.021 kb on + strand, within BBR_RS12995 at 686.021 kb on + strand, within BBR_RS12995 at 686.022 kb on + strand, within BBR_RS12995 at 686.050 kb on + strand, within BBR_RS12995 at 686.050 kb on + strand, within BBR_RS12995 at 686.050 kb on + strand, within BBR_RS12995 at 686.119 kb on + strand, within BBR_RS12995 at 686.327 kb on + strand, within BBR_RS12995 at 686.337 kb on + strand, within BBR_RS12995 at 686.421 kb on + strand at 686.442 kb on + strand at 686.442 kb on + strand at 686.442 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Trimethoprim 0.01 mM remove 683,714 - BBR_RS12985 0.33 -0.4 683,754 - BBR_RS12985 0.36 +1.0 683,771 - BBR_RS12985 0.37 -2.5 683,881 + BBR_RS12985 0.45 -2.6 683,917 + BBR_RS12985 0.48 -1.7 683,941 - BBR_RS12985 0.50 -1.2 683,965 - BBR_RS12985 0.52 -1.0 683,975 - BBR_RS12985 0.52 -1.8 684,010 + BBR_RS12985 0.55 -0.7 684,027 - BBR_RS12985 0.56 -2.2 684,039 - BBR_RS12985 0.57 -1.9 684,053 + BBR_RS12985 0.58 -1.4 684,059 + BBR_RS12985 0.59 -0.4 684,073 - BBR_RS12985 0.60 -1.2 684,074 + BBR_RS12985 0.60 -0.4 684,108 + BBR_RS12985 0.62 -1.5 684,116 - BBR_RS12985 0.63 -0.7 684,133 + BBR_RS12985 0.64 -1.3 684,147 + BBR_RS12985 0.65 -1.4 684,155 - BBR_RS12985 0.66 -0.8 684,165 + BBR_RS12985 0.67 -1.2 684,204 + BBR_RS12985 0.70 -2.1 684,283 + BBR_RS12985 0.76 -3.3 684,283 + BBR_RS12985 0.76 -2.5 684,323 - BBR_RS12985 0.79 +0.7 684,323 - BBR_RS12985 0.79 -2.3 684,341 - BBR_RS12985 0.80 -2.6 684,428 + BBR_RS12985 0.87 -1.1 684,428 + BBR_RS12985 0.87 -0.8 684,428 + BBR_RS12985 0.87 -1.9 684,431 - BBR_RS12985 0.87 -2.5 684,433 + BBR_RS12985 0.87 -1.1 684,441 - BBR_RS12985 0.88 -1.8 684,468 - BBR_RS12985 0.90 -1.8 684,497 - -1.2 684,497 - -2.0 684,550 + -1.8 684,550 + -1.3 684,550 + -0.8 684,552 + -0.5 684,558 - -2.5 684,573 - -1.5 684,584 - -1.6 684,599 + -0.9 684,599 + -1.7 684,607 - -0.0 684,607 - +0.4 684,694 + +0.0 684,735 + -0.4 684,749 + -0.8 684,749 + +0.4 684,803 + BBR_RS12990 0.15 -0.2 684,808 + BBR_RS12990 0.15 -0.1 684,831 - BBR_RS12990 0.18 -0.0 684,856 + BBR_RS12990 0.22 +0.6 684,874 - BBR_RS12990 0.24 -0.6 684,879 - BBR_RS12990 0.25 -0.8 684,887 - BBR_RS12990 0.26 -0.4 684,908 - BBR_RS12990 0.29 -0.1 684,925 + BBR_RS12990 0.31 -0.1 684,926 - BBR_RS12990 0.31 -0.2 684,929 - BBR_RS12990 0.31 -1.5 684,952 + BBR_RS12990 0.34 -0.8 684,960 - BBR_RS12990 0.35 -0.9 684,960 - BBR_RS12990 0.35 -0.8 684,960 - BBR_RS12990 0.35 -1.2 684,960 - BBR_RS12990 0.35 +0.1 684,990 + BBR_RS12990 0.39 +1.7 684,997 + BBR_RS12990 0.40 -0.2 685,015 - BBR_RS12990 0.43 +0.2 685,077 + BBR_RS12990 0.51 +0.5 685,091 + BBR_RS12990 0.53 -0.8 685,096 + BBR_RS12990 0.53 -1.5 685,105 + BBR_RS12990 0.55 -0.4 685,113 - BBR_RS12990 0.56 +0.0 685,113 - BBR_RS12990 0.56 -0.8 685,123 + BBR_RS12990 0.57 -0.3 685,131 - BBR_RS12990 0.58 -0.5 685,139 - BBR_RS12990 0.59 -0.2 685,145 + BBR_RS12990 0.60 -0.5 685,145 + BBR_RS12990 0.60 -0.0 685,145 + BBR_RS12990 0.60 -0.3 685,145 + BBR_RS12990 0.60 +0.9 685,145 + BBR_RS12990 0.60 +0.2 685,153 - BBR_RS12990 0.61 -1.3 685,153 - BBR_RS12990 0.61 +1.2 685,153 - BBR_RS12990 0.61 -1.1 685,178 - BBR_RS12990 0.64 +0.1 685,229 - BBR_RS12990 0.71 -0.7 685,249 + BBR_RS12990 0.74 -0.9 685,257 - BBR_RS12990 0.75 +0.7 685,280 - BBR_RS12990 0.78 -0.4 685,280 - BBR_RS12990 0.78 +0.9 685,289 - BBR_RS12990 0.79 -0.5 685,297 - BBR_RS12990 0.80 -0.1 685,409 + -0.6 685,412 - +0.8 685,412 - +0.7 685,434 + -0.5 685,456 - +0.2 685,517 + +0.4 685,520 + -0.6 685,541 + +0.1 685,549 + +0.4 685,549 + -0.5 685,551 + -0.1 685,557 - -0.0 685,701 + BBR_RS12995 0.16 -0.3 685,701 + BBR_RS12995 0.16 +0.2 685,738 + BBR_RS12995 0.20 +0.8 685,764 - BBR_RS12995 0.23 +0.0 685,765 + BBR_RS12995 0.23 -0.8 685,784 + BBR_RS12995 0.25 -0.8 685,855 + BBR_RS12995 0.33 +0.1 685,859 + BBR_RS12995 0.34 +0.9 685,976 + BBR_RS12995 0.47 -0.1 686,008 + BBR_RS12995 0.50 -0.8 686,014 + BBR_RS12995 0.51 -0.0 686,014 + BBR_RS12995 0.51 +0.3 686,014 + BBR_RS12995 0.51 -0.1 686,014 + BBR_RS12995 0.51 -0.6 686,021 + BBR_RS12995 0.52 -0.1 686,021 + BBR_RS12995 0.52 +1.1 686,022 + BBR_RS12995 0.52 -1.3 686,050 + BBR_RS12995 0.55 +0.3 686,050 + BBR_RS12995 0.55 -0.2 686,050 + BBR_RS12995 0.55 -2.1 686,119 + BBR_RS12995 0.62 -0.2 686,327 + BBR_RS12995 0.85 -0.1 686,337 + BBR_RS12995 0.87 +0.1 686,421 + +1.2 686,442 + -0.2 686,442 + +0.1 686,442 + -0.1
Or see this region's nucleotide sequence