Experiment: Recovery broth with Trimethoprim 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12415 and BBR_RS12420 are separated by 268 nucleotides BBR_RS12420 and BBR_RS12425 are separated by 91 nucleotides BBR_RS12425 and BBR_RS12430 are separated by 358 nucleotides BBR_RS12430 and BBR_RS12435 are separated by 603 nucleotides
BBR_RS12415: BBR_RS12415 - permease, at 533,953 to 534,954
_RS12415
BBR_RS12420: BBR_RS12420 - tRNA-Leu, at 535,223 to 535,303
_RS12420
BBR_RS12425: BBR_RS12425 - tRNA-Thr, at 535,395 to 535,470
_RS12425
BBR_RS12430: BBR_RS12430 - glucose-6-phosphate isomerase, at 535,829 to 537,529
_RS12430
BBR_RS12435: BBR_RS12435 - ABC transporter ATP-binding protein, at 538,133 to 539,551
_RS12435
Position (kb) 535
536
537
538 Strain fitness (log2 ratio) -2
-1
0
1
2 at 534.833 kb on + strand, within BBR_RS12415 at 534.833 kb on + strand, within BBR_RS12415 at 534.833 kb on + strand, within BBR_RS12415 at 534.841 kb on - strand, within BBR_RS12415 at 534.848 kb on - strand, within BBR_RS12415 at 534.890 kb on - strand at 534.903 kb on - strand at 534.908 kb on - strand at 534.948 kb on - strand at 534.950 kb on - strand at 534.963 kb on + strand at 535.023 kb on + strand at 535.031 kb on + strand at 535.031 kb on - strand at 535.063 kb on - strand at 535.157 kb on + strand at 535.210 kb on - strand at 535.211 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.316 kb on + strand at 535.319 kb on - strand at 535.391 kb on - strand at 535.391 kb on - strand at 535.391 kb on - strand at 535.633 kb on + strand at 535.641 kb on - strand at 535.668 kb on + strand at 535.676 kb on - strand at 535.678 kb on - strand at 535.696 kb on - strand at 535.775 kb on + strand at 537.575 kb on + strand at 537.580 kb on + strand at 537.593 kb on - strand at 537.641 kb on + strand at 537.649 kb on - strand at 537.649 kb on - strand at 537.772 kb on - strand at 537.800 kb on + strand at 537.859 kb on + strand at 537.859 kb on + strand at 537.866 kb on - strand at 537.879 kb on - strand at 537.903 kb on + strand at 537.910 kb on - strand at 537.932 kb on + strand at 537.951 kb on + strand at 537.963 kb on + strand at 537.971 kb on - strand at 537.971 kb on - strand at 538.027 kb on + strand at 538.027 kb on + strand at 538.030 kb on - strand at 538.035 kb on - strand at 538.035 kb on - strand at 538.045 kb on - strand at 538.063 kb on + strand at 538.063 kb on + strand at 538.082 kb on - strand at 538.082 kb on - strand at 538.105 kb on - strand at 538.140 kb on + strand at 538.148 kb on - strand at 538.148 kb on - strand at 538.214 kb on + strand at 538.216 kb on - strand at 538.226 kb on + strand at 538.232 kb on + strand at 538.234 kb on + strand at 538.257 kb on - strand at 538.292 kb on + strand, within BBR_RS12435 at 538.292 kb on + strand, within BBR_RS12435 at 538.313 kb on - strand, within BBR_RS12435 at 538.362 kb on - strand, within BBR_RS12435 at 538.370 kb on + strand, within BBR_RS12435 at 538.371 kb on + strand, within BBR_RS12435 at 538.393 kb on + strand, within BBR_RS12435 at 538.403 kb on - strand, within BBR_RS12435 at 538.435 kb on + strand, within BBR_RS12435 at 538.438 kb on - strand, within BBR_RS12435 at 538.438 kb on - strand, within BBR_RS12435 at 538.466 kb on + strand, within BBR_RS12435 at 538.469 kb on - strand, within BBR_RS12435 at 538.487 kb on - strand, within BBR_RS12435 at 538.487 kb on - strand, within BBR_RS12435
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Trimethoprim 0.01 mM remove 534,833 + BBR_RS12415 0.88 +0.3 534,833 + BBR_RS12415 0.88 -0.5 534,833 + BBR_RS12415 0.88 -0.2 534,841 - BBR_RS12415 0.89 +1.0 534,848 - BBR_RS12415 0.89 -0.4 534,890 - -0.3 534,903 - -0.4 534,908 - -0.6 534,948 - +0.1 534,950 - +0.6 534,963 + -0.1 535,023 + -0.1 535,031 + -0.3 535,031 - -0.8 535,063 - +1.0 535,157 + +0.3 535,210 - +0.8 535,211 + +0.2 535,212 + +0.5 535,212 + -0.2 535,212 + -0.0 535,212 + +0.1 535,212 + +0.7 535,212 + -0.1 535,212 + +0.2 535,212 + +1.8 535,212 + +1.0 535,212 + -0.1 535,212 + -0.4 535,220 - -0.1 535,220 - +0.5 535,220 - +1.0 535,220 - +0.2 535,220 - -0.5 535,220 - -0.1 535,220 - -0.4 535,316 + -0.6 535,319 - +0.1 535,391 - -0.5 535,391 - -0.0 535,391 - -0.0 535,633 + +0.4 535,641 - -0.5 535,668 + +0.1 535,676 - +0.2 535,678 - -0.1 535,696 - +0.2 535,775 + +1.1 537,575 + +1.1 537,580 + -0.8 537,593 - -0.6 537,641 + +0.5 537,649 - +0.5 537,649 - +0.2 537,772 - -1.4 537,800 + -0.6 537,859 + +0.2 537,859 + -0.3 537,866 - -0.1 537,879 - +0.2 537,903 + -0.4 537,910 - -0.4 537,932 + -0.2 537,951 + +0.2 537,963 + -0.3 537,971 - -1.1 537,971 - -0.2 538,027 + -0.4 538,027 + -0.8 538,030 - +0.3 538,035 - -0.5 538,035 - -0.1 538,045 - +0.1 538,063 + -1.1 538,063 + +0.0 538,082 - +1.5 538,082 - -0.6 538,105 - -2.0 538,140 + +0.7 538,148 - +0.3 538,148 - -1.0 538,214 + +0.5 538,216 - -0.7 538,226 + -2.2 538,232 + -1.4 538,234 + -0.9 538,257 - -0.9 538,292 + BBR_RS12435 0.11 -1.2 538,292 + BBR_RS12435 0.11 -0.8 538,313 - BBR_RS12435 0.13 -1.1 538,362 - BBR_RS12435 0.16 -0.8 538,370 + BBR_RS12435 0.17 -0.5 538,371 + BBR_RS12435 0.17 -2.3 538,393 + BBR_RS12435 0.18 -0.4 538,403 - BBR_RS12435 0.19 -0.4 538,435 + BBR_RS12435 0.21 +0.0 538,438 - BBR_RS12435 0.21 +0.2 538,438 - BBR_RS12435 0.21 -2.1 538,466 + BBR_RS12435 0.23 -0.7 538,469 - BBR_RS12435 0.24 -1.8 538,487 - BBR_RS12435 0.25 -1.2 538,487 - BBR_RS12435 0.25 -1.7
Or see this region's nucleotide sequence