Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS02115 and AZOBR_RS02120 are separated by 58 nucleotides AZOBR_RS02120 and AZOBR_RS02125 are separated by 213 nucleotides AZOBR_RS02125 and AZOBR_RS02130 are separated by 366 nucleotides AZOBR_RS02130 and AZOBR_RS02135 are separated by 77 nucleotides
AZOBR_RS02115: AZOBR_RS02115 - hypothetical protein, at 435,792 to 436,103
_RS02115
AZOBR_RS02120: AZOBR_RS02120 - hypothetical protein, at 436,162 to 436,635
_RS02120
AZOBR_RS02125: AZOBR_RS02125 - DNA mismatch repair protein MutS, at 436,849 to 439,551
_RS02125
AZOBR_RS02130: AZOBR_RS02130 - hypothetical protein, at 439,918 to 440,115
_RS02130
AZOBR_RS02135: AZOBR_RS02135 - hypothetical protein, at 440,193 to 440,705
_RS02135
Position (kb) 436
437
438
439
440 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 436.163 kb on + strand at 436.446 kb on - strand, within AZOBR_RS02120 at 436.763 kb on - strand at 436.763 kb on - strand at 436.799 kb on + strand at 436.800 kb on - strand at 436.960 kb on + strand at 436.978 kb on + strand at 437.104 kb on + strand at 437.105 kb on - strand at 437.452 kb on + strand, within AZOBR_RS02125 at 437.453 kb on - strand, within AZOBR_RS02125 at 437.453 kb on - strand, within AZOBR_RS02125 at 437.569 kb on + strand, within AZOBR_RS02125 at 437.570 kb on - strand, within AZOBR_RS02125 at 437.621 kb on - strand, within AZOBR_RS02125 at 438.200 kb on - strand, within AZOBR_RS02125 at 438.274 kb on + strand, within AZOBR_RS02125 at 438.274 kb on + strand, within AZOBR_RS02125 at 438.274 kb on + strand, within AZOBR_RS02125 at 438.275 kb on - strand, within AZOBR_RS02125 at 438.901 kb on + strand, within AZOBR_RS02125 at 438.901 kb on + strand, within AZOBR_RS02125 at 438.902 kb on - strand, within AZOBR_RS02125 at 439.126 kb on + strand, within AZOBR_RS02125 at 439.126 kb on + strand, within AZOBR_RS02125 at 439.127 kb on - strand, within AZOBR_RS02125 at 439.252 kb on + strand, within AZOBR_RS02125 at 439.252 kb on + strand, within AZOBR_RS02125 at 439.253 kb on - strand, within AZOBR_RS02125 at 439.525 kb on + strand at 439.560 kb on - strand at 439.663 kb on + strand at 439.664 kb on - strand at 439.664 kb on - strand at 439.677 kb on - strand at 439.875 kb on + strand at 439.875 kb on + strand at 439.875 kb on + strand at 439.875 kb on + strand at 439.876 kb on - strand at 439.876 kb on - strand at 439.876 kb on - strand at 439.876 kb on - strand at 439.919 kb on + strand at 439.919 kb on + strand at 439.919 kb on + strand at 439.920 kb on - strand at 439.920 kb on - strand at 439.920 kb on - strand at 439.920 kb on - strand at 440.356 kb on + strand, within AZOBR_RS02135 at 440.356 kb on + strand, within AZOBR_RS02135 at 440.356 kb on + strand, within AZOBR_RS02135 at 440.356 kb on + strand, within AZOBR_RS02135 at 440.357 kb on - strand, within AZOBR_RS02135
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 436,163 + +1.5 436,446 - AZOBR_RS02120 0.60 -0.1 436,763 - -0.2 436,763 - -0.0 436,799 + +0.8 436,800 - +0.6 436,960 + -0.2 436,978 + -0.3 437,104 + -0.1 437,105 - -0.5 437,452 + AZOBR_RS02125 0.22 -0.8 437,453 - AZOBR_RS02125 0.22 -0.6 437,453 - AZOBR_RS02125 0.22 +0.3 437,569 + AZOBR_RS02125 0.27 +0.4 437,570 - AZOBR_RS02125 0.27 +0.5 437,621 - AZOBR_RS02125 0.29 -0.3 438,200 - AZOBR_RS02125 0.50 +1.7 438,274 + AZOBR_RS02125 0.53 +0.2 438,274 + AZOBR_RS02125 0.53 -1.5 438,274 + AZOBR_RS02125 0.53 +1.0 438,275 - AZOBR_RS02125 0.53 +0.9 438,901 + AZOBR_RS02125 0.76 -0.4 438,901 + AZOBR_RS02125 0.76 -0.0 438,902 - AZOBR_RS02125 0.76 -1.2 439,126 + AZOBR_RS02125 0.84 -0.1 439,126 + AZOBR_RS02125 0.84 +0.8 439,127 - AZOBR_RS02125 0.84 +1.4 439,252 + AZOBR_RS02125 0.89 -0.7 439,252 + AZOBR_RS02125 0.89 +0.2 439,253 - AZOBR_RS02125 0.89 -1.6 439,525 + -0.2 439,560 - -0.3 439,663 + -0.3 439,664 - -0.2 439,664 - +0.1 439,677 - +0.1 439,875 + -3.8 439,875 + -0.9 439,875 + -2.5 439,875 + -2.6 439,876 - -0.9 439,876 - -0.9 439,876 - -0.2 439,876 - -1.2 439,919 + +0.6 439,919 + +0.6 439,919 + -0.6 439,920 - -0.2 439,920 - +0.5 439,920 - +0.2 439,920 - +1.4 440,356 + AZOBR_RS02135 0.32 -0.7 440,356 + AZOBR_RS02135 0.32 -1.3 440,356 + AZOBR_RS02135 0.32 -0.1 440,356 + AZOBR_RS02135 0.32 +1.0 440,357 - AZOBR_RS02135 0.32 +1.0
Or see this region's nucleotide sequence