Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01070 and AZOBR_RS01075 overlap by 1 nucleotides AZOBR_RS01075 and AZOBR_RS01080 are separated by 75 nucleotides AZOBR_RS01080 and AZOBR_RS01085 are separated by 309 nucleotides
AZOBR_RS01070: AZOBR_RS01070 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 218,753 to 219,247
_RS01070
AZOBR_RS01075: AZOBR_RS01075 - Rrf2 family transcriptional regulator, at 219,247 to 219,702
_RS01075
AZOBR_RS01080: AZOBR_RS01080 - cysteine synthase, at 219,778 to 220,737
_RS01080
AZOBR_RS01085: AZOBR_RS01085 - hypothetical protein, at 221,047 to 221,820
_RS01085
Position (kb) 219
220
221 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 219.693 kb on + strand at 220.702 kb on + strand at 220.702 kb on + strand at 220.702 kb on + strand at 220.703 kb on - strand at 220.736 kb on - strand at 220.790 kb on + strand at 220.791 kb on - strand at 220.819 kb on + strand at 220.844 kb on + strand at 220.844 kb on + strand at 220.845 kb on - strand at 220.924 kb on + strand at 221.141 kb on - strand, within AZOBR_RS01085 at 221.299 kb on + strand, within AZOBR_RS01085 at 221.300 kb on - strand, within AZOBR_RS01085 at 221.300 kb on - strand, within AZOBR_RS01085 at 221.300 kb on - strand, within AZOBR_RS01085 at 221.301 kb on + strand, within AZOBR_RS01085 at 221.302 kb on - strand, within AZOBR_RS01085 at 221.302 kb on - strand, within AZOBR_RS01085 at 221.348 kb on - strand, within AZOBR_RS01085 at 221.680 kb on + strand, within AZOBR_RS01085
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 219,693 + +0.6 220,702 + +0.8 220,702 + -0.5 220,702 + +0.3 220,703 - -2.7 220,736 - -0.4 220,790 + +2.0 220,791 - +0.4 220,819 + -0.5 220,844 + +0.1 220,844 + -0.2 220,845 - +0.4 220,924 + +0.2 221,141 - AZOBR_RS01085 0.12 -3.1 221,299 + AZOBR_RS01085 0.33 -4.0 221,300 - AZOBR_RS01085 0.33 -3.4 221,300 - AZOBR_RS01085 0.33 -1.2 221,300 - AZOBR_RS01085 0.33 -2.4 221,301 + AZOBR_RS01085 0.33 -4.9 221,302 - AZOBR_RS01085 0.33 -3.5 221,302 - AZOBR_RS01085 0.33 -3.5 221,348 - AZOBR_RS01085 0.39 -3.0 221,680 + AZOBR_RS01085 0.82 -2.7
Or see this region's nucleotide sequence