Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_77 and Pf1N1B4_78 are separated by 143 nucleotides Pf1N1B4_78 and Pf1N1B4_79 are separated by 179 nucleotides Pf1N1B4_79 and Pf1N1B4_80 are separated by 18 nucleotides
Pf1N1B4_77 - FIG00956064: hypothetical protein, at 81,000 to 81,317
_77
Pf1N1B4_78 - FIG00956937: hypothetical protein, at 81,461 to 81,994
_78
Pf1N1B4_79 - Lactoylglutathione lyase and related lyases, at 82,174 to 82,542
_79
Pf1N1B4_80 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 82,561 to 83,844
_80
Position (kb) 82
83 Strain fitness (log2 ratio) -2
-1
0
1
2 at 81.220 kb on + strand, within Pf1N1B4_77 at 81.289 kb on + strand at 81.290 kb on - strand at 81.290 kb on - strand at 81.315 kb on + strand at 81.316 kb on - strand at 81.334 kb on + strand at 81.335 kb on - strand at 81.335 kb on - strand at 81.403 kb on - strand at 81.403 kb on - strand at 81.426 kb on + strand at 81.778 kb on - strand, within Pf1N1B4_78 at 81.778 kb on - strand, within Pf1N1B4_78 at 81.778 kb on - strand, within Pf1N1B4_78 at 81.778 kb on - strand, within Pf1N1B4_78 at 81.967 kb on + strand at 81.968 kb on - strand at 82.022 kb on + strand at 82.064 kb on + strand at 82.064 kb on + strand at 82.064 kb on + strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.070 kb on + strand at 82.071 kb on - strand at 82.071 kb on - strand at 82.071 kb on - strand at 82.090 kb on + strand at 82.090 kb on + strand at 82.091 kb on - strand at 82.091 kb on - strand at 82.091 kb on - strand at 82.124 kb on + strand at 82.125 kb on - strand at 82.324 kb on + strand, within Pf1N1B4_79 at 82.324 kb on + strand, within Pf1N1B4_79 at 82.325 kb on - strand, within Pf1N1B4_79 at 82.357 kb on + strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.492 kb on + strand, within Pf1N1B4_79 at 82.526 kb on + strand at 82.526 kb on + strand at 82.526 kb on + strand at 82.527 kb on - strand at 82.527 kb on - strand at 82.527 kb on - strand at 82.527 kb on - strand at 82.540 kb on + strand at 82.564 kb on + strand at 82.564 kb on + strand at 82.564 kb on + strand at 82.565 kb on - strand at 82.565 kb on - strand at 82.688 kb on + strand at 82.688 kb on + strand at 82.689 kb on - strand at 82.719 kb on - strand, within Pf1N1B4_80 at 82.744 kb on + strand, within Pf1N1B4_80 at 82.744 kb on + strand, within Pf1N1B4_80 at 82.745 kb on - strand, within Pf1N1B4_80 at 83.040 kb on + strand, within Pf1N1B4_80 at 83.041 kb on - strand, within Pf1N1B4_80 at 83.165 kb on + strand, within Pf1N1B4_80 at 83.166 kb on - strand, within Pf1N1B4_80 at 83.364 kb on + strand, within Pf1N1B4_80 at 83.364 kb on + strand at 83.365 kb on - strand, within Pf1N1B4_80 at 83.365 kb on - strand, within Pf1N1B4_80 at 83.366 kb on + strand, within Pf1N1B4_80 at 83.423 kb on - strand, within Pf1N1B4_80 at 83.423 kb on - strand, within Pf1N1B4_80 at 83.450 kb on + strand, within Pf1N1B4_80
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 81,220 + Pf1N1B4_77 0.69 -0.0 81,289 + -0.9 81,290 - -0.5 81,290 - -0.5 81,315 + +1.2 81,316 - -1.9 81,334 + -1.9 81,335 - +0.0 81,335 - +0.2 81,403 - +1.3 81,403 - -0.2 81,426 + -0.4 81,778 - Pf1N1B4_78 0.59 +0.0 81,778 - Pf1N1B4_78 0.59 +1.0 81,778 - Pf1N1B4_78 0.59 +0.2 81,778 - Pf1N1B4_78 0.59 +0.6 81,967 + -2.3 81,968 - +0.0 82,022 + -0.6 82,064 + +0.3 82,064 + +0.9 82,064 + +0.2 82,065 - +0.2 82,065 - +0.5 82,065 - -0.2 82,065 - -1.4 82,065 - +0.5 82,070 + -1.5 82,071 - -0.1 82,071 - +0.2 82,071 - -0.8 82,090 + +0.3 82,090 + +0.7 82,091 - -1.1 82,091 - -0.5 82,091 - -1.5 82,124 + +0.8 82,125 - +0.6 82,324 + Pf1N1B4_79 0.41 +0.4 82,324 + Pf1N1B4_79 0.41 +0.2 82,325 - Pf1N1B4_79 0.41 +0.5 82,357 + Pf1N1B4_79 0.50 +0.7 82,358 - Pf1N1B4_79 0.50 +0.2 82,358 - Pf1N1B4_79 0.50 +0.4 82,358 - Pf1N1B4_79 0.50 -0.6 82,358 - Pf1N1B4_79 0.50 +0.4 82,358 - Pf1N1B4_79 0.50 +0.7 82,492 + Pf1N1B4_79 0.86 +1.8 82,526 + -0.7 82,526 + -0.2 82,526 + -0.5 82,527 - +1.0 82,527 - -0.4 82,527 - -0.1 82,527 - -2.0 82,540 + +0.6 82,564 + -0.5 82,564 + -0.1 82,564 + -0.8 82,565 - +0.2 82,565 - -1.1 82,688 + -0.5 82,688 + -0.4 82,689 - -1.3 82,719 - Pf1N1B4_80 0.12 +0.5 82,744 + Pf1N1B4_80 0.14 +0.8 82,744 + Pf1N1B4_80 0.14 -0.6 82,745 - Pf1N1B4_80 0.14 +0.0 83,040 + Pf1N1B4_80 0.37 -0.1 83,041 - Pf1N1B4_80 0.37 -0.2 83,165 + Pf1N1B4_80 0.47 +0.0 83,166 - Pf1N1B4_80 0.47 +0.8 83,364 + Pf1N1B4_80 0.63 +0.2 83,364 + +0.8 83,365 - Pf1N1B4_80 0.63 -0.4 83,365 - Pf1N1B4_80 0.63 -0.3 83,366 + Pf1N1B4_80 0.63 +0.2 83,423 - Pf1N1B4_80 0.67 +0.3 83,423 - Pf1N1B4_80 0.67 +0.0 83,450 + Pf1N1B4_80 0.69 -0.3
Or see this region's nucleotide sequence