Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_70 and Pf1N1B4_71 are separated by 23 nucleotides Pf1N1B4_71 and Pf1N1B4_72 are separated by 123 nucleotides
Pf1N1B4_70 - Major facilitator family transporter, at 72,931 to 74,256
_70
Pf1N1B4_71 - Glucarate dehydratase (EC 4.2.1.40), at 74,280 to 75,623
_71
Pf1N1B4_72 - Transcriptional regulator, LysR family, at 75,747 to 76,619
_72
Position (kb) 74
75
76 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 73.318 kb on + strand at 73.319 kb on - strand, within Pf1N1B4_70 at 73.319 kb on - strand, within Pf1N1B4_70 at 73.321 kb on + strand, within Pf1N1B4_70 at 73.321 kb on + strand, within Pf1N1B4_70 at 73.322 kb on - strand, within Pf1N1B4_70 at 73.392 kb on - strand at 73.471 kb on + strand, within Pf1N1B4_70 at 73.471 kb on + strand at 73.471 kb on + strand, within Pf1N1B4_70 at 73.472 kb on - strand, within Pf1N1B4_70 at 73.472 kb on - strand, within Pf1N1B4_70 at 73.472 kb on - strand at 73.690 kb on + strand, within Pf1N1B4_70 at 73.690 kb on + strand, within Pf1N1B4_70 at 73.690 kb on + strand, within Pf1N1B4_70 at 73.690 kb on + strand, within Pf1N1B4_70 at 73.691 kb on - strand, within Pf1N1B4_70 at 73.691 kb on - strand, within Pf1N1B4_70 at 73.715 kb on - strand, within Pf1N1B4_70 at 73.825 kb on + strand, within Pf1N1B4_70 at 73.825 kb on + strand, within Pf1N1B4_70 at 73.825 kb on + strand, within Pf1N1B4_70 at 73.825 kb on + strand, within Pf1N1B4_70 at 73.826 kb on - strand, within Pf1N1B4_70 at 73.826 kb on - strand, within Pf1N1B4_70 at 73.826 kb on - strand, within Pf1N1B4_70 at 73.826 kb on - strand, within Pf1N1B4_70 at 74.015 kb on - strand, within Pf1N1B4_70 at 74.244 kb on + strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.268 kb on - strand at 74.465 kb on + strand, within Pf1N1B4_71 at 74.523 kb on + strand, within Pf1N1B4_71 at 74.742 kb on + strand, within Pf1N1B4_71 at 74.742 kb on + strand, within Pf1N1B4_71 at 74.742 kb on + strand, within Pf1N1B4_71 at 74.742 kb on + strand, within Pf1N1B4_71 at 74.743 kb on - strand, within Pf1N1B4_71 at 74.743 kb on - strand, within Pf1N1B4_71 at 75.206 kb on - strand, within Pf1N1B4_71 at 75.210 kb on - strand, within Pf1N1B4_71 at 75.320 kb on + strand, within Pf1N1B4_71 at 75.523 kb on - strand at 75.523 kb on - strand at 75.523 kb on - strand at 75.523 kb on - strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.746 kb on - strand at 75.746 kb on - strand at 75.746 kb on - strand at 75.746 kb on - strand at 75.749 kb on - strand at 75.749 kb on - strand at 75.829 kb on + strand at 76.251 kb on + strand, within Pf1N1B4_72 at 76.251 kb on + strand, within Pf1N1B4_72 at 76.252 kb on - strand, within Pf1N1B4_72 at 76.252 kb on - strand, within Pf1N1B4_72
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 73,318 + +0.8 73,319 - Pf1N1B4_70 0.29 -1.6 73,319 - Pf1N1B4_70 0.29 +0.8 73,321 + Pf1N1B4_70 0.29 +0.5 73,321 + Pf1N1B4_70 0.29 -1.6 73,322 - Pf1N1B4_70 0.29 +1.8 73,392 - +1.5 73,471 + Pf1N1B4_70 0.41 +1.2 73,471 + -0.8 73,471 + Pf1N1B4_70 0.41 +0.3 73,472 - Pf1N1B4_70 0.41 -0.2 73,472 - Pf1N1B4_70 0.41 -0.7 73,472 - -1.0 73,690 + Pf1N1B4_70 0.57 +0.8 73,690 + Pf1N1B4_70 0.57 -0.7 73,690 + Pf1N1B4_70 0.57 +0.0 73,690 + Pf1N1B4_70 0.57 -0.8 73,691 - Pf1N1B4_70 0.57 +0.1 73,691 - Pf1N1B4_70 0.57 +0.0 73,715 - Pf1N1B4_70 0.59 -0.5 73,825 + Pf1N1B4_70 0.67 -0.4 73,825 + Pf1N1B4_70 0.67 -0.4 73,825 + Pf1N1B4_70 0.67 -0.1 73,825 + Pf1N1B4_70 0.67 +1.4 73,826 - Pf1N1B4_70 0.67 +1.8 73,826 - Pf1N1B4_70 0.67 -0.8 73,826 - Pf1N1B4_70 0.67 -0.7 73,826 - Pf1N1B4_70 0.67 +2.3 74,015 - Pf1N1B4_70 0.82 -1.0 74,244 + -0.3 74,245 - -0.7 74,245 - -0.6 74,245 - +0.7 74,245 - -1.3 74,245 - -0.3 74,245 - -1.3 74,268 - +0.8 74,465 + Pf1N1B4_71 0.14 -0.3 74,523 + Pf1N1B4_71 0.18 -1.7 74,742 + Pf1N1B4_71 0.34 +0.1 74,742 + Pf1N1B4_71 0.34 -0.9 74,742 + Pf1N1B4_71 0.34 -0.2 74,742 + Pf1N1B4_71 0.34 -1.5 74,743 - Pf1N1B4_71 0.34 -0.3 74,743 - Pf1N1B4_71 0.34 -0.3 75,206 - Pf1N1B4_71 0.69 -1.1 75,210 - Pf1N1B4_71 0.69 -0.5 75,320 + Pf1N1B4_71 0.77 -0.8 75,523 - +0.0 75,523 - +0.3 75,523 - +0.8 75,523 - -3.0 75,745 + +0.8 75,745 + +0.9 75,745 + +0.3 75,745 + -0.9 75,745 + -1.4 75,746 - +0.4 75,746 - -0.5 75,746 - -0.0 75,746 - +0.2 75,749 - -0.4 75,749 - +0.3 75,829 + -0.3 76,251 + Pf1N1B4_72 0.58 -0.5 76,251 + Pf1N1B4_72 0.58 -0.5 76,252 - Pf1N1B4_72 0.58 +2.2 76,252 - Pf1N1B4_72 0.58 -0.2
Or see this region's nucleotide sequence