Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5797 and Pf1N1B4_5798 are separated by 191 nucleotides Pf1N1B4_5798 and Pf1N1B4_5799 are separated by 10 nucleotides Pf1N1B4_5799 and Pf1N1B4_5800 overlap by 4 nucleotides
Pf1N1B4_5797 - 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase (EC 1.3.1.25), at 232,800 to 233,573
_5797
Pf1N1B4_5798 - 2-chlorobenzoate 1,2-dioxygenase reductase component, at 233,765 to 234,778
_5798
Pf1N1B4_5799 - Benzoate 1,2-dioxygenase beta subunit (EC 1.14.12.10), at 234,789 to 235,277
_5799
Pf1N1B4_5800 - Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10), at 235,274 to 236,638
_5800
Position (kb) 233
234
235 Strain fitness (log2 ratio) -2
-1
0
1
2 at 232.846 kb on + strand at 232.847 kb on - strand at 232.847 kb on - strand at 232.847 kb on - strand at 232.847 kb on - strand at 233.380 kb on + strand, within Pf1N1B4_5797 at 233.409 kb on + strand, within Pf1N1B4_5797 at 233.409 kb on + strand, within Pf1N1B4_5797 at 233.410 kb on - strand, within Pf1N1B4_5797 at 233.577 kb on + strand at 233.578 kb on - strand at 233.605 kb on + strand at 233.606 kb on - strand at 233.606 kb on - strand at 233.628 kb on + strand at 233.629 kb on - strand at 233.685 kb on + strand at 233.797 kb on - strand at 233.874 kb on + strand, within Pf1N1B4_5798 at 233.928 kb on + strand, within Pf1N1B4_5798 at 234.171 kb on - strand, within Pf1N1B4_5798 at 234.175 kb on - strand, within Pf1N1B4_5798 at 234.175 kb on - strand, within Pf1N1B4_5798 at 234.357 kb on + strand at 234.357 kb on + strand, within Pf1N1B4_5798 at 234.358 kb on - strand, within Pf1N1B4_5798 at 234.358 kb on - strand, within Pf1N1B4_5798 at 234.460 kb on - strand, within Pf1N1B4_5798 at 234.591 kb on + strand at 234.591 kb on + strand, within Pf1N1B4_5798 at 234.591 kb on + strand, within Pf1N1B4_5798 at 234.592 kb on - strand, within Pf1N1B4_5798 at 234.800 kb on - strand at 234.824 kb on - strand at 234.824 kb on - strand at 234.824 kb on - strand at 234.890 kb on - strand, within Pf1N1B4_5799 at 234.890 kb on - strand, within Pf1N1B4_5799 at 234.907 kb on + strand, within Pf1N1B4_5799 at 234.907 kb on + strand, within Pf1N1B4_5799 at 234.907 kb on + strand, within Pf1N1B4_5799 at 234.907 kb on + strand, within Pf1N1B4_5799 at 234.908 kb on - strand, within Pf1N1B4_5799 at 235.186 kb on + strand at 235.268 kb on + strand at 235.268 kb on + strand at 235.269 kb on - strand at 235.269 kb on - strand at 235.269 kb on - strand at 235.347 kb on + strand at 235.347 kb on + strand at 235.348 kb on - strand at 235.632 kb on + strand, within Pf1N1B4_5800 at 235.633 kb on - strand, within Pf1N1B4_5800 at 235.633 kb on - strand, within Pf1N1B4_5800 at 235.707 kb on + strand, within Pf1N1B4_5800
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 232,846 + -0.6 232,847 - -0.7 232,847 - -0.3 232,847 - -0.1 232,847 - +0.6 233,380 + Pf1N1B4_5797 0.75 -1.9 233,409 + Pf1N1B4_5797 0.79 -0.7 233,409 + Pf1N1B4_5797 0.79 -0.0 233,410 - Pf1N1B4_5797 0.79 +0.1 233,577 + +1.1 233,578 - -1.1 233,605 + +0.3 233,606 - +1.0 233,606 - -0.2 233,628 + +0.1 233,629 - -0.1 233,685 + -0.2 233,797 - +2.1 233,874 + Pf1N1B4_5798 0.11 +0.3 233,928 + Pf1N1B4_5798 0.16 -1.2 234,171 - Pf1N1B4_5798 0.40 -0.9 234,175 - Pf1N1B4_5798 0.40 -0.3 234,175 - Pf1N1B4_5798 0.40 +0.1 234,357 + +0.4 234,357 + Pf1N1B4_5798 0.58 -0.5 234,358 - Pf1N1B4_5798 0.58 -0.5 234,358 - Pf1N1B4_5798 0.58 -0.1 234,460 - Pf1N1B4_5798 0.69 -1.6 234,591 + +2.2 234,591 + Pf1N1B4_5798 0.81 -0.3 234,591 + Pf1N1B4_5798 0.81 -0.6 234,592 - Pf1N1B4_5798 0.82 -1.7 234,800 - -0.9 234,824 - -1.3 234,824 - -0.2 234,824 - +0.1 234,890 - Pf1N1B4_5799 0.21 +0.6 234,890 - Pf1N1B4_5799 0.21 -0.5 234,907 + Pf1N1B4_5799 0.24 +0.3 234,907 + Pf1N1B4_5799 0.24 +0.4 234,907 + Pf1N1B4_5799 0.24 -0.7 234,907 + Pf1N1B4_5799 0.24 +0.9 234,908 - Pf1N1B4_5799 0.24 -0.1 235,186 + -1.3 235,268 + -1.2 235,268 + -0.2 235,269 - -1.0 235,269 - -0.6 235,269 - +0.1 235,347 + -0.0 235,347 + -0.9 235,348 - -0.3 235,632 + Pf1N1B4_5800 0.26 -2.7 235,633 - Pf1N1B4_5800 0.26 -1.1 235,633 - Pf1N1B4_5800 0.26 -0.7 235,707 + Pf1N1B4_5800 0.32 -0.5
Or see this region's nucleotide sequence