Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5321 and Pf1N1B4_5322 are separated by 744 nucleotides Pf1N1B4_5322 and Pf1N1B4_5323 are separated by 96 nucleotides
Pf1N1B4_5321 - hypothetical protein, at 70,641 to 70,970
_5321
Pf1N1B4_5322 - hypothetical protein, at 71,715 to 71,900
_5322
Pf1N1B4_5323 - hypothetical protein, at 71,997 to 72,953
_5323
Position (kb) 71
72 Strain fitness (log2 ratio) -1
0
1
2 at 70.722 kb on - strand, within Pf1N1B4_5321 at 70.722 kb on - strand, within Pf1N1B4_5321 at 70.722 kb on - strand, within Pf1N1B4_5321 at 70.824 kb on - strand, within Pf1N1B4_5321 at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.059 kb on - strand at 71.059 kb on - strand at 71.059 kb on - strand at 71.092 kb on + strand at 71.092 kb on + strand at 71.093 kb on - strand at 71.114 kb on + strand at 71.114 kb on + strand at 71.115 kb on - strand at 71.131 kb on - strand at 71.139 kb on - strand at 71.229 kb on + strand at 71.335 kb on + strand at 71.335 kb on + strand at 71.336 kb on - strand at 71.356 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.433 kb on + strand at 71.437 kb on + strand at 71.437 kb on + strand at 71.437 kb on + strand at 71.481 kb on - strand at 71.499 kb on + strand at 71.669 kb on + strand at 71.669 kb on + strand at 71.669 kb on + strand at 71.669 kb on + strand at 71.670 kb on - strand at 71.670 kb on - strand at 71.715 kb on - strand at 71.752 kb on + strand, within Pf1N1B4_5322 at 71.753 kb on - strand, within Pf1N1B4_5322 at 71.763 kb on - strand, within Pf1N1B4_5322 at 71.880 kb on + strand, within Pf1N1B4_5322 at 71.880 kb on + strand, within Pf1N1B4_5322 at 71.898 kb on + strand at 71.899 kb on - strand at 71.913 kb on + strand at 71.917 kb on + strand at 71.917 kb on + strand at 71.918 kb on - strand at 71.926 kb on + strand at 71.926 kb on + strand at 71.927 kb on - strand at 71.975 kb on + strand at 72.165 kb on - strand, within Pf1N1B4_5323 at 72.268 kb on + strand, within Pf1N1B4_5323 at 72.269 kb on - strand, within Pf1N1B4_5323 at 72.411 kb on - strand, within Pf1N1B4_5323 at 72.495 kb on + strand, within Pf1N1B4_5323 at 72.496 kb on - strand, within Pf1N1B4_5323 at 72.496 kb on - strand, within Pf1N1B4_5323 at 72.496 kb on - strand, within Pf1N1B4_5323 at 72.515 kb on - strand, within Pf1N1B4_5323 at 72.529 kb on + strand, within Pf1N1B4_5323 at 72.529 kb on + strand, within Pf1N1B4_5323 at 72.530 kb on - strand, within Pf1N1B4_5323 at 72.531 kb on + strand, within Pf1N1B4_5323 at 72.531 kb on + strand, within Pf1N1B4_5323 at 72.531 kb on + strand, within Pf1N1B4_5323 at 72.532 kb on - strand, within Pf1N1B4_5323 at 72.575 kb on - strand, within Pf1N1B4_5323 at 72.668 kb on + strand, within Pf1N1B4_5323 at 72.788 kb on + strand, within Pf1N1B4_5323 at 72.865 kb on + strand at 72.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 70,722 - Pf1N1B4_5321 0.25 +0.7 70,722 - Pf1N1B4_5321 0.25 +2.0 70,722 - Pf1N1B4_5321 0.25 -0.6 70,824 - Pf1N1B4_5321 0.55 -0.8 71,058 + +0.3 71,058 + +0.4 71,058 + -0.6 71,058 + +0.7 71,058 + +0.7 71,058 + +0.2 71,059 - +0.2 71,059 - +0.0 71,059 - -0.1 71,092 + -0.4 71,092 + +0.7 71,093 - -0.5 71,114 + -0.9 71,114 + -0.0 71,115 - +1.3 71,131 - -1.5 71,139 - +0.5 71,229 + +0.5 71,335 + +0.3 71,335 + +0.7 71,336 - +0.7 71,356 + -0.2 71,358 + -1.0 71,358 + -0.2 71,358 + +0.6 71,358 + -0.8 71,358 + -0.1 71,358 + +0.5 71,358 + -1.0 71,359 - -0.5 71,359 - -1.2 71,359 - +0.7 71,359 - +1.0 71,359 - -0.2 71,359 - +0.7 71,433 + +1.2 71,437 + -0.7 71,437 + -1.0 71,437 + -0.3 71,481 - +0.0 71,499 + -0.3 71,669 + +0.4 71,669 + +0.2 71,669 + -0.2 71,669 + +0.2 71,670 - -0.1 71,670 - +1.7 71,715 - +1.2 71,752 + Pf1N1B4_5322 0.20 -0.5 71,753 - Pf1N1B4_5322 0.20 -1.5 71,763 - Pf1N1B4_5322 0.26 -1.3 71,880 + Pf1N1B4_5322 0.89 -1.4 71,880 + Pf1N1B4_5322 0.89 -0.7 71,898 + -1.2 71,899 - -0.1 71,913 + +0.4 71,917 + -0.6 71,917 + -0.6 71,918 - +0.9 71,926 + +0.2 71,926 + -1.3 71,927 - -0.0 71,975 + +1.5 72,165 - Pf1N1B4_5323 0.18 -1.1 72,268 + Pf1N1B4_5323 0.28 +0.0 72,269 - Pf1N1B4_5323 0.28 -0.2 72,411 - Pf1N1B4_5323 0.43 -0.4 72,495 + Pf1N1B4_5323 0.52 -0.1 72,496 - Pf1N1B4_5323 0.52 +0.8 72,496 - Pf1N1B4_5323 0.52 +0.8 72,496 - Pf1N1B4_5323 0.52 -0.7 72,515 - Pf1N1B4_5323 0.54 +0.1 72,529 + Pf1N1B4_5323 0.56 -0.5 72,529 + Pf1N1B4_5323 0.56 +0.3 72,530 - Pf1N1B4_5323 0.56 +2.5 72,531 + Pf1N1B4_5323 0.56 +0.4 72,531 + Pf1N1B4_5323 0.56 +0.7 72,531 + Pf1N1B4_5323 0.56 +0.3 72,532 - Pf1N1B4_5323 0.56 -0.7 72,575 - Pf1N1B4_5323 0.60 -0.8 72,668 + Pf1N1B4_5323 0.70 -0.8 72,788 + Pf1N1B4_5323 0.83 -0.1 72,865 + -1.1 72,866 - +1.7
Or see this region's nucleotide sequence