Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4611 and Pf1N1B4_4612 are separated by 30 nucleotides Pf1N1B4_4612 and Pf1N1B4_4613 overlap by 4 nucleotides Pf1N1B4_4613 and Pf1N1B4_4614 are separated by 10 nucleotides
Pf1N1B4_4611 - GlpG protein (membrane protein of glp regulon), at 5,080,901 to 5,081,773
_4611
Pf1N1B4_4612 - FIG00954117: hypothetical protein, at 5,081,804 to 5,082,064
_4612
Pf1N1B4_4613 - FIG00953934: hypothetical protein, at 5,082,061 to 5,082,891
_4613
Pf1N1B4_4614 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 5,082,902 to 5,085,559
_4614
Position (kb)
5082
5083 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5081.136 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.744 kb on + strand at 5081.744 kb on + strand at 5081.745 kb on - strand at 5081.772 kb on - strand at 5081.776 kb on + strand at 5081.776 kb on + strand at 5081.777 kb on - strand at 5081.777 kb on - strand at 5081.777 kb on - strand at 5081.849 kb on + strand, within Pf1N1B4_4612 at 5081.849 kb on + strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.987 kb on + strand at 5081.988 kb on - strand, within Pf1N1B4_4612 at 5082.197 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.578 kb on + strand, within Pf1N1B4_4613 at 5082.855 kb on + strand at 5082.859 kb on + strand at 5082.859 kb on + strand at 5082.860 kb on - strand at 5082.902 kb on - strand at 5083.140 kb on - strand at 5083.247 kb on + strand, within Pf1N1B4_4614 at 5083.248 kb on - strand, within Pf1N1B4_4614 at 5083.308 kb on - strand at 5083.337 kb on - strand, within Pf1N1B4_4614 at 5083.345 kb on + strand, within Pf1N1B4_4614 at 5083.559 kb on + strand, within Pf1N1B4_4614 at 5083.560 kb on - strand at 5083.791 kb on + strand at 5083.792 kb on - strand, within Pf1N1B4_4614
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 5,081,136 + Pf1N1B4_4611 0.27 -0.7 5,081,477 + Pf1N1B4_4611 0.66 -0.7 5,081,477 + Pf1N1B4_4611 0.66 +0.4 5,081,477 + Pf1N1B4_4611 0.66 -0.1 5,081,477 + Pf1N1B4_4611 0.66 -0.7 5,081,477 + Pf1N1B4_4611 0.66 +0.0 5,081,744 + -0.2 5,081,744 + -0.7 5,081,745 - +0.0 5,081,772 - -0.2 5,081,776 + -0.4 5,081,776 + +1.5 5,081,777 - -1.0 5,081,777 - -0.6 5,081,777 - +0.9 5,081,849 + Pf1N1B4_4612 0.17 -1.8 5,081,849 + Pf1N1B4_4612 0.17 -1.4 5,081,850 - Pf1N1B4_4612 0.18 +0.4 5,081,850 - Pf1N1B4_4612 0.18 +0.2 5,081,850 - Pf1N1B4_4612 0.18 -2.8 5,081,987 + -0.3 5,081,988 - Pf1N1B4_4612 0.70 -0.6 5,082,197 - Pf1N1B4_4613 0.16 -0.4 5,082,329 - Pf1N1B4_4613 0.32 -1.1 5,082,329 - Pf1N1B4_4613 0.32 -1.2 5,082,578 + Pf1N1B4_4613 0.62 -0.1 5,082,855 + -0.1 5,082,859 + +0.8 5,082,859 + -0.3 5,082,860 - +0.7 5,082,902 - -0.3 5,083,140 - -1.3 5,083,247 + Pf1N1B4_4614 0.13 -0.0 5,083,248 - Pf1N1B4_4614 0.13 +0.6 5,083,308 - +1.0 5,083,337 - Pf1N1B4_4614 0.16 -0.9 5,083,345 + Pf1N1B4_4614 0.17 +1.0 5,083,559 + Pf1N1B4_4614 0.25 -0.7 5,083,560 - -1.3 5,083,791 + -1.3 5,083,792 - Pf1N1B4_4614 0.33 +0.1
Or see this region's nucleotide sequence