Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4002

Experiment: LB 10C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4001 and Pf1N1B4_4002 are separated by 201 nucleotidesPf1N1B4_4002 and Pf1N1B4_4003 are separated by 126 nucleotidesPf1N1B4_4003 and Pf1N1B4_4004 overlap by 4 nucleotides Pf1N1B4_4001 - Membrane fusion component of tripartite multidrug resistance system, at 4,449,471 to 4,450,673 _4001 Pf1N1B4_4002 - Inner membrane component of tripartite multidrug resistance system, at 4,450,875 to 4,452,404 _4002 Pf1N1B4_4003 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 4,452,531 to 4,453,283 _4003 Pf1N1B4_4004 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 4,453,280 to 4,453,783 _4004 Position (kb) 4450 4451 4452 4453Strain fitness (log2 ratio) -2 -1 0 1at 4450.329 kb on + strand, within Pf1N1B4_4001at 4450.329 kb on + strand, within Pf1N1B4_4001at 4450.330 kb on - strand, within Pf1N1B4_4001at 4450.584 kb on + strandat 4450.584 kb on + strandat 4450.584 kb on + strandat 4450.584 kb on + strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4451.233 kb on - strand, within Pf1N1B4_4002at 4451.367 kb on + strand, within Pf1N1B4_4002at 4451.367 kb on + strand, within Pf1N1B4_4002at 4451.368 kb on - strandat 4451.368 kb on - strand, within Pf1N1B4_4002at 4451.481 kb on + strand, within Pf1N1B4_4002at 4451.481 kb on + strand, within Pf1N1B4_4002at 4451.482 kb on - strand, within Pf1N1B4_4002at 4451.853 kb on + strandat 4451.854 kb on - strand, within Pf1N1B4_4002at 4452.162 kb on + strand, within Pf1N1B4_4002at 4452.162 kb on + strand, within Pf1N1B4_4002at 4452.162 kb on + strand, within Pf1N1B4_4002at 4452.162 kb on + strand, within Pf1N1B4_4002at 4452.162 kb on + strand, within Pf1N1B4_4002at 4452.162 kb on + strand, within Pf1N1B4_4002at 4452.163 kb on - strand, within Pf1N1B4_4002at 4452.163 kb on - strand, within Pf1N1B4_4002at 4452.163 kb on - strand, within Pf1N1B4_4002at 4452.163 kb on - strand, within Pf1N1B4_4002at 4452.179 kb on + strand, within Pf1N1B4_4002at 4452.186 kb on + strand, within Pf1N1B4_4002at 4452.186 kb on + strand, within Pf1N1B4_4002at 4452.279 kb on + strandat 4452.279 kb on + strandat 4452.279 kb on + strandat 4452.279 kb on + strandat 4452.279 kb on + strandat 4452.300 kb on + strandat 4452.301 kb on - strandat 4453.326 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 10C
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4,450,329 + Pf1N1B4_4001 0.71 -0.0
4,450,329 + Pf1N1B4_4001 0.71 +0.3
4,450,330 - Pf1N1B4_4001 0.71 -0.9
4,450,584 + +0.1
4,450,584 + +0.1
4,450,584 + +0.3
4,450,584 + -0.4
4,450,585 - -0.2
4,450,585 - -0.4
4,450,585 - -1.8
4,450,585 - +0.4
4,450,585 - -1.2
4,450,585 - -0.7
4,451,233 - Pf1N1B4_4002 0.23 +0.5
4,451,367 + Pf1N1B4_4002 0.32 -0.3
4,451,367 + Pf1N1B4_4002 0.32 -1.4
4,451,368 - -1.0
4,451,368 - Pf1N1B4_4002 0.32 -1.2
4,451,481 + Pf1N1B4_4002 0.40 -0.0
4,451,481 + Pf1N1B4_4002 0.40 -0.2
4,451,482 - Pf1N1B4_4002 0.40 +0.1
4,451,853 + -0.2
4,451,854 - Pf1N1B4_4002 0.64 +0.1
4,452,162 + Pf1N1B4_4002 0.84 +0.7
4,452,162 + Pf1N1B4_4002 0.84 -1.1
4,452,162 + Pf1N1B4_4002 0.84 -0.8
4,452,162 + Pf1N1B4_4002 0.84 +1.3
4,452,162 + Pf1N1B4_4002 0.84 -0.0
4,452,162 + Pf1N1B4_4002 0.84 -0.2
4,452,163 - Pf1N1B4_4002 0.84 +0.3
4,452,163 - Pf1N1B4_4002 0.84 -0.6
4,452,163 - Pf1N1B4_4002 0.84 +0.5
4,452,163 - Pf1N1B4_4002 0.84 +0.7
4,452,179 + Pf1N1B4_4002 0.85 -0.2
4,452,186 + Pf1N1B4_4002 0.86 +0.2
4,452,186 + Pf1N1B4_4002 0.86 -0.0
4,452,279 + -0.4
4,452,279 + +0.4
4,452,279 + +0.9
4,452,279 + +0.4
4,452,279 + +0.4
4,452,300 + -0.4
4,452,301 - +0.2
4,453,326 - +0.8

Or see this region's nucleotide sequence