Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_314

Experiment: LB 10C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_312 and Pf1N1B4_313 are separated by 151 nucleotidesPf1N1B4_313 and Pf1N1B4_314 are separated by 200 nucleotidesPf1N1B4_314 and Pf1N1B4_315 are separated by 10 nucleotides Pf1N1B4_312 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 365,145 to 367,796 _312 Pf1N1B4_313 - Potassium-transporting ATPase C chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 367,948 to 368,493 _313 Pf1N1B4_314 - Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 368,694 to 370,751 _314 Pf1N1B4_315 - Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 370,762 to 372,456 _315 Position (kb) 368 369 370 371Strain fitness (log2 ratio) -2 -1 0 1at 367.725 kb on + strandat 367.725 kb on + strandat 367.725 kb on + strandat 367.813 kb on - strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.895 kb on + strandat 367.896 kb on - strandat 367.896 kb on - strandat 367.896 kb on - strandat 367.949 kb on + strandat 367.968 kb on + strandat 368.046 kb on + strand, within Pf1N1B4_313at 368.047 kb on - strand, within Pf1N1B4_313at 368.047 kb on - strand, within Pf1N1B4_313at 368.177 kb on - strand, within Pf1N1B4_313at 368.323 kb on + strand, within Pf1N1B4_313at 368.426 kb on + strand, within Pf1N1B4_313at 368.479 kb on + strandat 368.479 kb on + strandat 368.480 kb on - strandat 368.506 kb on + strandat 368.506 kb on + strandat 368.506 kb on + strandat 368.507 kb on - strandat 368.698 kb on - strandat 368.774 kb on - strandat 368.774 kb on - strandat 368.948 kb on - strand, within Pf1N1B4_314at 368.983 kb on + strand, within Pf1N1B4_314at 368.983 kb on + strand, within Pf1N1B4_314at 368.983 kb on + strand, within Pf1N1B4_314at 368.983 kb on + strand, within Pf1N1B4_314at 368.983 kb on + strand, within Pf1N1B4_314at 368.984 kb on - strand, within Pf1N1B4_314at 368.984 kb on - strand, within Pf1N1B4_314at 369.040 kb on - strand, within Pf1N1B4_314at 369.673 kb on + strand, within Pf1N1B4_314at 369.769 kb on + strand, within Pf1N1B4_314at 369.770 kb on - strandat 370.262 kb on + strand, within Pf1N1B4_314at 370.651 kb on - strandat 370.734 kb on - strandat 370.763 kb on + strandat 370.763 kb on + strandat 370.764 kb on - strandat 370.764 kb on - strandat 370.959 kb on - strand, within Pf1N1B4_315at 370.959 kb on - strand, within Pf1N1B4_315at 370.959 kb on - strand, within Pf1N1B4_315at 371.066 kb on + strand, within Pf1N1B4_315at 371.066 kb on + strand, within Pf1N1B4_315at 371.067 kb on - strand, within Pf1N1B4_315at 371.067 kb on - strand, within Pf1N1B4_315at 371.067 kb on - strand, within Pf1N1B4_315at 371.067 kb on - strand, within Pf1N1B4_315at 371.067 kb on - strand, within Pf1N1B4_315at 371.072 kb on + strand, within Pf1N1B4_315at 371.073 kb on - strand, within Pf1N1B4_315at 371.073 kb on - strand, within Pf1N1B4_315at 371.073 kb on - strand, within Pf1N1B4_315at 371.235 kb on - strand, within Pf1N1B4_315at 371.273 kb on - strand, within Pf1N1B4_315at 371.303 kb on + strand, within Pf1N1B4_315at 371.304 kb on - strand, within Pf1N1B4_315at 371.538 kb on - strand, within Pf1N1B4_315at 371.627 kb on + strand, within Pf1N1B4_315at 371.627 kb on + strand, within Pf1N1B4_315at 371.627 kb on + strand, within Pf1N1B4_315at 371.628 kb on - strand, within Pf1N1B4_315at 371.628 kb on - strand, within Pf1N1B4_315at 371.628 kb on - strand, within Pf1N1B4_315

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 10C
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367,725 + -1.5
367,725 + +0.9
367,725 + +0.8
367,813 - +1.0
367,895 + -1.4
367,895 + +0.1
367,895 + -0.6
367,895 + -1.1
367,895 + -0.6
367,895 + +0.5
367,895 + -0.7
367,895 + +1.0
367,895 + -1.8
367,895 + -0.9
367,895 + +1.3
367,895 + +0.2
367,895 + +0.2
367,895 + +1.2
367,895 + -0.4
367,895 + -1.5
367,896 - -0.5
367,896 - -0.6
367,896 - -0.5
367,949 + -0.1
367,968 + +0.0
368,046 + Pf1N1B4_313 0.18 +0.7
368,047 - Pf1N1B4_313 0.18 +0.3
368,047 - Pf1N1B4_313 0.18 -0.5
368,177 - Pf1N1B4_313 0.42 -0.1
368,323 + Pf1N1B4_313 0.69 -0.8
368,426 + Pf1N1B4_313 0.88 -0.6
368,479 + +1.4
368,479 + -0.1
368,480 - -0.7
368,506 + +1.4
368,506 + -0.3
368,506 + -1.1
368,507 - -0.1
368,698 - -0.1
368,774 - -0.5
368,774 - +0.3
368,948 - Pf1N1B4_314 0.12 -0.8
368,983 + Pf1N1B4_314 0.14 -1.3
368,983 + Pf1N1B4_314 0.14 -0.8
368,983 + Pf1N1B4_314 0.14 -1.2
368,983 + Pf1N1B4_314 0.14 -0.7
368,983 + Pf1N1B4_314 0.14 -1.4
368,984 - Pf1N1B4_314 0.14 -0.9
368,984 - Pf1N1B4_314 0.14 -0.7
369,040 - Pf1N1B4_314 0.17 -0.4
369,673 + Pf1N1B4_314 0.48 -0.6
369,769 + Pf1N1B4_314 0.52 -1.4
369,770 - -1.1
370,262 + Pf1N1B4_314 0.76 -0.1
370,651 - +0.5
370,734 - -1.2
370,763 + -0.7
370,763 + +0.3
370,764 - +0.1
370,764 - +0.0
370,959 - Pf1N1B4_315 0.12 -0.9
370,959 - Pf1N1B4_315 0.12 -0.7
370,959 - Pf1N1B4_315 0.12 +0.2
371,066 + Pf1N1B4_315 0.18 -0.6
371,066 + Pf1N1B4_315 0.18 +0.3
371,067 - Pf1N1B4_315 0.18 -0.7
371,067 - Pf1N1B4_315 0.18 +0.3
371,067 - Pf1N1B4_315 0.18 +0.2
371,067 - Pf1N1B4_315 0.18 +0.4
371,067 - Pf1N1B4_315 0.18 -0.6
371,072 + Pf1N1B4_315 0.18 -2.4
371,073 - Pf1N1B4_315 0.18 -0.8
371,073 - Pf1N1B4_315 0.18 +0.2
371,073 - Pf1N1B4_315 0.18 +1.0
371,235 - Pf1N1B4_315 0.28 -0.5
371,273 - Pf1N1B4_315 0.30 -1.1
371,303 + Pf1N1B4_315 0.32 -0.7
371,304 - Pf1N1B4_315 0.32 +0.9
371,538 - Pf1N1B4_315 0.46 +0.4
371,627 + Pf1N1B4_315 0.51 +0.5
371,627 + Pf1N1B4_315 0.51 -0.2
371,627 + Pf1N1B4_315 0.51 -1.4
371,628 - Pf1N1B4_315 0.51 -0.3
371,628 - Pf1N1B4_315 0.51 +0.3
371,628 - Pf1N1B4_315 0.51 -0.6

Or see this region's nucleotide sequence