Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01750 and IAI47_01755 are separated by 227 nucleotides IAI47_01755 and IAI47_01760 are separated by 29 nucleotides IAI47_01760 and IAI47_01765 are separated by 30 nucleotides
IAI47_01750: IAI47_01750 - glycerol-3-phosphate dehydrogenase, at 382,339 to 383,844
_01750
IAI47_01755: IAI47_01755 - thiosulfate sulfurtransferase GlpE, at 384,072 to 384,398
_01755
IAI47_01760: IAI47_01760 - rhomboid family intramembrane serine protease GlpG, at 384,428 to 385,249
_01760
IAI47_01765: IAI47_01765 - DeoR/GlpR family transcriptional regulator, at 385,280 to 386,038
_01765
Position (kb) 384
385 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 383.089 kb on - strand, within IAI47_01750 at 383.107 kb on - strand, within IAI47_01750 at 383.107 kb on - strand, within IAI47_01750 at 383.108 kb on + strand, within IAI47_01750 at 383.109 kb on - strand, within IAI47_01750 at 383.109 kb on - strand, within IAI47_01750 at 383.109 kb on - strand, within IAI47_01750 at 383.109 kb on - strand, within IAI47_01750 at 383.109 kb on - strand, within IAI47_01750 at 383.109 kb on - strand, within IAI47_01750 at 383.109 kb on - strand, within IAI47_01750 at 383.109 kb on - strand, within IAI47_01750 at 383.109 kb on - strand, within IAI47_01750 at 383.146 kb on - strand, within IAI47_01750 at 383.146 kb on - strand, within IAI47_01750 at 383.146 kb on - strand, within IAI47_01750 at 383.175 kb on - strand, within IAI47_01750 at 383.424 kb on - strand, within IAI47_01750 at 383.424 kb on - strand, within IAI47_01750 at 383.523 kb on - strand, within IAI47_01750 at 383.523 kb on - strand, within IAI47_01750 at 383.523 kb on - strand, within IAI47_01750 at 383.523 kb on - strand, within IAI47_01750 at 383.682 kb on - strand, within IAI47_01750 at 383.958 kb on + strand at 383.959 kb on - strand at 383.960 kb on + strand at 383.960 kb on + strand at 383.960 kb on + strand at 383.960 kb on + strand at 383.961 kb on - strand at 383.961 kb on - strand at 383.961 kb on - strand at 383.961 kb on - strand at 383.961 kb on - strand at 383.961 kb on - strand at 383.964 kb on + strand at 383.964 kb on + strand at 383.965 kb on - strand at 383.965 kb on - strand at 383.965 kb on - strand at 383.991 kb on + strand at 383.992 kb on - strand at 383.992 kb on - strand at 383.997 kb on + strand at 384.025 kb on + strand at 384.033 kb on + strand at 384.033 kb on + strand at 384.033 kb on + strand at 384.034 kb on - strand at 384.034 kb on - strand at 384.038 kb on + strand at 384.039 kb on - strand at 384.045 kb on + strand at 384.045 kb on + strand at 384.046 kb on - strand at 384.059 kb on + strand at 384.059 kb on + strand at 384.059 kb on + strand at 384.059 kb on + strand at 384.059 kb on + strand at 384.059 kb on + strand at 384.059 kb on + strand at 384.060 kb on - strand at 384.060 kb on - strand at 384.089 kb on + strand at 384.089 kb on + strand at 384.089 kb on + strand at 384.089 kb on + strand at 384.089 kb on + strand at 384.089 kb on + strand at 384.089 kb on + strand at 384.089 kb on + strand at 384.089 kb on + strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.090 kb on - strand at 384.162 kb on + strand, within IAI47_01755 at 384.162 kb on + strand, within IAI47_01755 at 384.162 kb on + strand, within IAI47_01755 at 384.162 kb on + strand, within IAI47_01755 at 384.163 kb on - strand, within IAI47_01755 at 384.163 kb on - strand, within IAI47_01755 at 384.163 kb on - strand, within IAI47_01755 at 384.163 kb on - strand, within IAI47_01755 at 384.163 kb on - strand, within IAI47_01755 at 384.163 kb on - strand, within IAI47_01755 at 384.264 kb on + strand, within IAI47_01755 at 384.264 kb on + strand, within IAI47_01755 at 384.264 kb on + strand, within IAI47_01755 at 384.265 kb on - strand, within IAI47_01755 at 384.265 kb on - strand, within IAI47_01755 at 384.265 kb on - strand, within IAI47_01755 at 384.265 kb on - strand, within IAI47_01755 at 384.265 kb on - strand, within IAI47_01755 at 384.273 kb on - strand, within IAI47_01755 at 384.273 kb on - strand, within IAI47_01755 at 384.275 kb on + strand, within IAI47_01755 at 384.275 kb on + strand, within IAI47_01755 at 384.275 kb on + strand, within IAI47_01755 at 384.276 kb on - strand, within IAI47_01755 at 384.276 kb on - strand, within IAI47_01755 at 384.276 kb on - strand, within IAI47_01755 at 384.297 kb on + strand, within IAI47_01755 at 384.298 kb on - strand, within IAI47_01755 at 384.327 kb on + strand, within IAI47_01755 at 384.328 kb on - strand, within IAI47_01755 at 384.396 kb on + strand at 384.396 kb on + strand at 384.396 kb on + strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.423 kb on + strand at 384.423 kb on + strand at 384.423 kb on + strand at 384.423 kb on + strand at 384.423 kb on + strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.437 kb on - strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.480 kb on - strand at 384.480 kb on - strand at 384.480 kb on - strand at 384.480 kb on - strand at 384.480 kb on - strand at 384.527 kb on + strand, within IAI47_01760 at 384.528 kb on - strand, within IAI47_01760 at 384.528 kb on - strand, within IAI47_01760 at 384.528 kb on - strand, within IAI47_01760 at 384.662 kb on + strand, within IAI47_01760 at 384.663 kb on - strand, within IAI47_01760 at 384.676 kb on + strand, within IAI47_01760 at 384.676 kb on + strand, within IAI47_01760 at 384.676 kb on + strand, within IAI47_01760 at 384.677 kb on - strand, within IAI47_01760 at 384.677 kb on - strand, within IAI47_01760 at 384.748 kb on + strand, within IAI47_01760 at 384.748 kb on + strand, within IAI47_01760 at 384.749 kb on - strand, within IAI47_01760 at 384.749 kb on - strand, within IAI47_01760 at 384.749 kb on - strand, within IAI47_01760 at 384.880 kb on + strand, within IAI47_01760 at 384.881 kb on - strand, within IAI47_01760 at 384.899 kb on + strand, within IAI47_01760 at 384.899 kb on + strand, within IAI47_01760 at 384.899 kb on + strand, within IAI47_01760 at 384.899 kb on + strand, within IAI47_01760 at 384.900 kb on - strand, within IAI47_01760 at 384.900 kb on - strand, within IAI47_01760 at 384.900 kb on - strand, within IAI47_01760 at 384.900 kb on - strand, within IAI47_01760 at 384.948 kb on + strand, within IAI47_01760 at 384.948 kb on + strand, within IAI47_01760 at 384.948 kb on + strand at 384.948 kb on + strand, within IAI47_01760 at 384.949 kb on - strand, within IAI47_01760 at 384.949 kb on - strand, within IAI47_01760 at 384.949 kb on - strand, within IAI47_01760 at 384.953 kb on - strand, within IAI47_01760 at 384.953 kb on - strand, within IAI47_01760 at 384.953 kb on - strand, within IAI47_01760 at 384.953 kb on - strand, within IAI47_01760 at 385.034 kb on + strand, within IAI47_01760 at 385.034 kb on + strand, within IAI47_01760 at 385.035 kb on - strand, within IAI47_01760 at 385.050 kb on - strand, within IAI47_01760 at 385.091 kb on + strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.247 kb on + strand at 385.317 kb on + strand at 385.317 kb on + strand at 385.318 kb on - strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.342 kb on + strand at 385.356 kb on + strand, within IAI47_01765 at 385.356 kb on + strand, within IAI47_01765 at 385.356 kb on + strand, within IAI47_01765 at 385.356 kb on + strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 383,089 - IAI47_01750 0.50 -0.1 383,107 - IAI47_01750 0.51 +0.8 383,107 - IAI47_01750 0.51 +2.2 383,108 + IAI47_01750 0.51 +1.9 383,109 - IAI47_01750 0.51 -1.1 383,109 - IAI47_01750 0.51 -0.9 383,109 - IAI47_01750 0.51 +4.9 383,109 - IAI47_01750 0.51 +1.3 383,109 - IAI47_01750 0.51 -1.7 383,109 - IAI47_01750 0.51 -2.5 383,109 - IAI47_01750 0.51 -0.4 383,109 - IAI47_01750 0.51 +0.4 383,109 - IAI47_01750 0.51 +0.0 383,146 - IAI47_01750 0.54 +0.6 383,146 - IAI47_01750 0.54 +2.0 383,146 - IAI47_01750 0.54 +0.9 383,175 - IAI47_01750 0.56 -1.0 383,424 - IAI47_01750 0.72 -2.0 383,424 - IAI47_01750 0.72 +0.7 383,523 - IAI47_01750 0.79 +0.9 383,523 - IAI47_01750 0.79 +1.2 383,523 - IAI47_01750 0.79 +2.6 383,523 - IAI47_01750 0.79 +0.9 383,682 - IAI47_01750 0.89 -0.2 383,958 + -0.2 383,959 - +1.3 383,960 + -0.1 383,960 + +1.0 383,960 + +0.6 383,960 + -0.8 383,961 - -3.1 383,961 - -1.9 383,961 - -0.7 383,961 - +0.3 383,961 - +0.0 383,961 - -0.9 383,964 + +2.6 383,964 + -3.3 383,965 - -1.7 383,965 - -2.3 383,965 - +0.4 383,991 + -0.1 383,992 - +2.5 383,992 - +0.6 383,997 + -0.8 384,025 + +1.0 384,033 + +0.5 384,033 + -0.5 384,033 + +0.8 384,034 - -0.4 384,034 - +0.1 384,038 + +0.3 384,039 - +0.5 384,045 + +0.4 384,045 + -0.0 384,046 - -0.8 384,059 + +1.6 384,059 + +2.0 384,059 + +2.0 384,059 + -0.2 384,059 + +1.4 384,059 + +0.0 384,059 + +0.8 384,060 - -0.7 384,060 - +1.1 384,089 + -0.9 384,089 + -0.4 384,089 + -0.4 384,089 + -1.0 384,089 + +1.4 384,089 + +0.3 384,089 + -1.2 384,089 + +1.6 384,089 + -1.8 384,090 - -0.7 384,090 - -0.2 384,090 - -0.4 384,090 - +1.0 384,090 - +0.3 384,090 - -0.7 384,090 - -1.5 384,090 - +0.4 384,090 - -0.2 384,090 - +0.2 384,090 - +0.9 384,090 - +0.0 384,090 - +0.6 384,090 - +1.5 384,090 - +0.8 384,090 - -1.7 384,162 + IAI47_01755 0.28 -3.0 384,162 + IAI47_01755 0.28 +0.5 384,162 + IAI47_01755 0.28 -0.6 384,162 + IAI47_01755 0.28 -0.7 384,163 - IAI47_01755 0.28 -2.4 384,163 - IAI47_01755 0.28 -0.3 384,163 - IAI47_01755 0.28 -3.3 384,163 - IAI47_01755 0.28 -0.4 384,163 - IAI47_01755 0.28 -0.8 384,163 - IAI47_01755 0.28 +0.0 384,264 + IAI47_01755 0.59 +0.8 384,264 + IAI47_01755 0.59 +0.0 384,264 + IAI47_01755 0.59 -0.8 384,265 - IAI47_01755 0.59 -1.5 384,265 - IAI47_01755 0.59 +0.1 384,265 - IAI47_01755 0.59 -1.0 384,265 - IAI47_01755 0.59 +0.4 384,265 - IAI47_01755 0.59 -1.6 384,273 - IAI47_01755 0.61 +2.9 384,273 - IAI47_01755 0.61 +0.3 384,275 + IAI47_01755 0.62 -1.1 384,275 + IAI47_01755 0.62 +0.0 384,275 + IAI47_01755 0.62 -0.8 384,276 - IAI47_01755 0.62 -0.8 384,276 - IAI47_01755 0.62 +1.0 384,276 - IAI47_01755 0.62 -1.0 384,297 + IAI47_01755 0.69 +0.3 384,298 - IAI47_01755 0.69 +0.2 384,327 + IAI47_01755 0.78 -1.5 384,328 - IAI47_01755 0.78 -0.6 384,396 + -0.9 384,396 + -1.3 384,396 + -0.0 384,397 - -0.2 384,397 - +1.9 384,397 - -2.3 384,397 - +1.4 384,397 - -0.3 384,397 - -2.6 384,397 - +0.7 384,423 + +1.2 384,423 + -1.5 384,423 + -0.9 384,423 + -2.1 384,423 + +1.0 384,424 - -0.1 384,424 - -2.4 384,424 - -0.8 384,424 - +0.3 384,424 - -2.2 384,424 - -2.0 384,424 - -0.5 384,437 - +2.0 384,479 + +0.9 384,479 + +0.6 384,479 + -1.4 384,479 + -1.3 384,479 + +1.9 384,479 + +1.2 384,479 + -2.2 384,479 + +0.0 384,479 + +0.4 384,479 + +1.3 384,479 + +1.0 384,479 + +0.3 384,480 - -0.7 384,480 - -4.3 384,480 - -1.8 384,480 - -1.2 384,480 - +1.3 384,527 + IAI47_01760 0.12 +0.7 384,528 - IAI47_01760 0.12 -2.7 384,528 - IAI47_01760 0.12 +1.0 384,528 - IAI47_01760 0.12 +2.0 384,662 + IAI47_01760 0.28 +2.3 384,663 - IAI47_01760 0.29 +1.6 384,676 + IAI47_01760 0.30 -0.6 384,676 + IAI47_01760 0.30 -1.3 384,676 + IAI47_01760 0.30 -0.5 384,677 - IAI47_01760 0.30 +0.6 384,677 - IAI47_01760 0.30 -0.9 384,748 + IAI47_01760 0.39 -1.2 384,748 + IAI47_01760 0.39 +1.3 384,749 - IAI47_01760 0.39 -1.0 384,749 - IAI47_01760 0.39 -2.1 384,749 - IAI47_01760 0.39 +1.0 384,880 + IAI47_01760 0.55 +0.4 384,881 - IAI47_01760 0.55 +2.0 384,899 + IAI47_01760 0.57 +0.2 384,899 + IAI47_01760 0.57 +1.6 384,899 + IAI47_01760 0.57 -1.7 384,899 + IAI47_01760 0.57 +1.1 384,900 - IAI47_01760 0.57 -3.4 384,900 - IAI47_01760 0.57 +0.1 384,900 - IAI47_01760 0.57 -1.5 384,900 - IAI47_01760 0.57 +1.5 384,948 + IAI47_01760 0.63 -1.2 384,948 + IAI47_01760 0.63 +0.9 384,948 + +1.6 384,948 + IAI47_01760 0.63 -0.8 384,949 - IAI47_01760 0.63 +0.6 384,949 - IAI47_01760 0.63 -0.4 384,949 - IAI47_01760 0.63 -0.3 384,953 - IAI47_01760 0.64 -0.3 384,953 - IAI47_01760 0.64 +0.5 384,953 - IAI47_01760 0.64 -2.0 384,953 - IAI47_01760 0.64 +0.8 385,034 + IAI47_01760 0.74 -0.3 385,034 + IAI47_01760 0.74 -2.4 385,035 - IAI47_01760 0.74 +0.5 385,050 - IAI47_01760 0.76 -1.7 385,091 + IAI47_01760 0.81 +2.2 385,092 - IAI47_01760 0.81 +0.8 385,092 - IAI47_01760 0.81 +5.7 385,092 - IAI47_01760 0.81 +0.2 385,092 - IAI47_01760 0.81 -0.0 385,092 - IAI47_01760 0.81 -2.8 385,247 + +2.4 385,317 + -0.1 385,317 + -0.5 385,318 - -2.0 385,334 + -0.2 385,334 + +1.6 385,334 + +0.0 385,334 + -2.1 385,334 + -1.2 385,334 + -0.5 385,334 + +3.2 385,334 + -0.4 385,335 - -0.3 385,335 - +0.0 385,335 - -1.4 385,335 - +0.6 385,335 - +1.6 385,335 - -3.6 385,335 - -1.1 385,335 - +1.8 385,342 + +1.3 385,356 + IAI47_01765 0.10 +0.0 385,356 + IAI47_01765 0.10 +0.2 385,356 + IAI47_01765 0.10 +2.5 385,356 + IAI47_01765 0.10 -1.6 385,357 - IAI47_01765 0.10 +1.0 385,357 - IAI47_01765 0.10 -3.1 385,357 - IAI47_01765 0.10 -0.2 385,357 - IAI47_01765 0.10 -0.7 385,357 - IAI47_01765 0.10 -0.4 385,357 - IAI47_01765 0.10 -2.3
Or see this region's nucleotide sequence