Experiment: ALP with Cisplatin 0.0009 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2191 and Dsui_2192 overlap by 16 nucleotides Dsui_2192 and Dsui_2193 are separated by 10 nucleotides Dsui_2193 and Dsui_2194 are separated by 131 nucleotides
Dsui_2191: Dsui_2191 - squalene-associated FAD-dependent desaturase, at 2,327,343 to 2,328,680
_2191
Dsui_2192: Dsui_2192 - squalene synthase HpnD, at 2,328,665 to 2,329,498
_2192
Dsui_2193: Dsui_2193 - squalene synthase HpnC, at 2,329,509 to 2,330,333
_2193
Dsui_2194: Dsui_2194 - tRNA-Leu, at 2,330,465 to 2,330,549
_2194
Position (kb)
2328
2329
2330 Strain fitness (log2 ratio)
-1
0
1
2 at 2328.155 kb on + strand at 2328.336 kb on - strand, within Dsui_2191 at 2328.487 kb on - strand at 2328.519 kb on - strand, within Dsui_2191 at 2328.519 kb on - strand at 2328.728 kb on - strand at 2328.742 kb on + strand at 2328.772 kb on + strand at 2328.797 kb on - strand, within Dsui_2192 at 2328.797 kb on - strand, within Dsui_2192 at 2328.853 kb on - strand, within Dsui_2192 at 2328.861 kb on + strand at 2329.108 kb on - strand, within Dsui_2192 at 2329.116 kb on - strand, within Dsui_2192 at 2329.123 kb on + strand, within Dsui_2192 at 2329.131 kb on - strand, within Dsui_2192 at 2329.177 kb on + strand at 2329.186 kb on - strand, within Dsui_2192 at 2329.248 kb on + strand, within Dsui_2192 at 2329.255 kb on - strand, within Dsui_2192 at 2329.276 kb on - strand, within Dsui_2192 at 2329.292 kb on - strand, within Dsui_2192 at 2329.406 kb on - strand, within Dsui_2192 at 2329.600 kb on + strand at 2329.753 kb on + strand, within Dsui_2193 at 2329.761 kb on - strand, within Dsui_2193 at 2329.761 kb on - strand, within Dsui_2193 at 2329.761 kb on - strand, within Dsui_2193 at 2329.780 kb on - strand, within Dsui_2193 at 2329.780 kb on - strand, within Dsui_2193 at 2329.780 kb on - strand, within Dsui_2193 at 2329.780 kb on - strand, within Dsui_2193 at 2329.856 kb on + strand at 2329.859 kb on - strand, within Dsui_2193 at 2329.859 kb on - strand, within Dsui_2193 at 2329.927 kb on - strand, within Dsui_2193 at 2329.990 kb on + strand at 2330.013 kb on - strand, within Dsui_2193 at 2330.209 kb on - strand, within Dsui_2193 at 2330.213 kb on - strand, within Dsui_2193 at 2330.266 kb on - strand at 2330.337 kb on - strand at 2330.368 kb on + strand at 2330.423 kb on + strand at 2330.423 kb on - strand at 2330.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0009 mg/ml remove 2,328,155 + -1.4 2,328,336 - Dsui_2191 0.74 -0.7 2,328,487 - -0.1 2,328,519 - Dsui_2191 0.88 -0.1 2,328,519 - +0.9 2,328,728 - -0.3 2,328,742 + -0.1 2,328,772 + +0.9 2,328,797 - Dsui_2192 0.16 +0.5 2,328,797 - Dsui_2192 0.16 +0.1 2,328,853 - Dsui_2192 0.23 -0.3 2,328,861 + -0.1 2,329,108 - Dsui_2192 0.53 +0.4 2,329,116 - Dsui_2192 0.54 +0.3 2,329,123 + Dsui_2192 0.55 -1.4 2,329,131 - Dsui_2192 0.56 +0.1 2,329,177 + -0.7 2,329,186 - Dsui_2192 0.62 +0.9 2,329,248 + Dsui_2192 0.70 +0.9 2,329,255 - Dsui_2192 0.71 -0.4 2,329,276 - Dsui_2192 0.73 -0.3 2,329,292 - Dsui_2192 0.75 -0.5 2,329,406 - Dsui_2192 0.89 -0.4 2,329,600 + +0.9 2,329,753 + Dsui_2193 0.30 +0.9 2,329,761 - Dsui_2193 0.31 +1.9 2,329,761 - Dsui_2193 0.31 +1.2 2,329,761 - Dsui_2193 0.31 -0.8 2,329,780 - Dsui_2193 0.33 +1.9 2,329,780 - Dsui_2193 0.33 +1.2 2,329,780 - Dsui_2193 0.33 +0.5 2,329,780 - Dsui_2193 0.33 +2.7 2,329,856 + +0.9 2,329,859 - Dsui_2193 0.42 -0.7 2,329,859 - Dsui_2193 0.42 -1.1 2,329,927 - Dsui_2193 0.51 -0.1 2,329,990 + -0.1 2,330,013 - Dsui_2193 0.61 +0.9 2,330,209 - Dsui_2193 0.85 +2.2 2,330,213 - Dsui_2193 0.85 -0.9 2,330,266 - -0.1 2,330,337 - -0.1 2,330,368 + -1.4 2,330,423 + -0.7 2,330,423 - -0.2 2,330,430 - +0.6
Or see this region's nucleotide sequence