Experiment: ALP with Cisplatin 0.0009 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2187 and Dsui_2188 are separated by 320 nucleotides Dsui_2188 and Dsui_2189 are separated by 145 nucleotides Dsui_2189 and Dsui_2190 are separated by 316 nucleotides Dsui_2190 and Dsui_2191 are separated by 10 nucleotides
Dsui_2187: Dsui_2187 - acyl-CoA synthetase/AMP-acid ligase, at 2,321,416 to 2,323,113
_2187
Dsui_2188: Dsui_2188 - Protein of unknown function (DUF3460), at 2,323,434 to 2,323,625
_2188
Dsui_2189: Dsui_2189 - acyl-CoA synthetase (NDP forming), at 2,323,771 to 2,326,461
_2189
Dsui_2190: Dsui_2190 - Tellurite resistance protein TehB, at 2,326,778 to 2,327,332
_2190
Dsui_2191: Dsui_2191 - squalene-associated FAD-dependent desaturase, at 2,327,343 to 2,328,680
_2191
Position (kb)
2323
2324
2325
2326
2327 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2322.782 kb on - strand at 2322.804 kb on + strand, within Dsui_2187 at 2322.850 kb on - strand, within Dsui_2187 at 2322.906 kb on - strand at 2323.017 kb on + strand at 2323.034 kb on + strand at 2323.048 kb on + strand at 2323.096 kb on + strand at 2323.176 kb on - strand at 2323.329 kb on + strand at 2323.431 kb on + strand at 2323.450 kb on - strand at 2323.457 kb on + strand, within Dsui_2188 at 2323.547 kb on + strand, within Dsui_2188 at 2323.587 kb on + strand, within Dsui_2188 at 2323.595 kb on + strand, within Dsui_2188 at 2323.619 kb on - strand at 2323.640 kb on + strand at 2323.669 kb on + strand at 2323.669 kb on - strand at 2323.749 kb on + strand at 2324.195 kb on - strand, within Dsui_2189 at 2324.280 kb on - strand, within Dsui_2189 at 2324.420 kb on + strand at 2324.474 kb on - strand, within Dsui_2189 at 2324.764 kb on - strand at 2324.974 kb on - strand, within Dsui_2189 at 2325.069 kb on + strand at 2325.199 kb on + strand at 2325.290 kb on + strand at 2325.736 kb on - strand, within Dsui_2189 at 2325.754 kb on - strand, within Dsui_2189 at 2325.827 kb on - strand, within Dsui_2189 at 2325.836 kb on - strand, within Dsui_2189 at 2325.847 kb on - strand, within Dsui_2189 at 2326.085 kb on - strand, within Dsui_2189 at 2326.087 kb on - strand, within Dsui_2189 at 2326.087 kb on - strand, within Dsui_2189 at 2326.222 kb on - strand at 2326.222 kb on - strand at 2326.456 kb on + strand at 2326.584 kb on + strand at 2326.773 kb on - strand at 2326.842 kb on - strand at 2326.842 kb on - strand at 2327.039 kb on - strand at 2327.071 kb on + strand at 2327.088 kb on + strand, within Dsui_2190 at 2327.111 kb on + strand, within Dsui_2190 at 2327.135 kb on - strand at 2327.294 kb on + strand at 2327.334 kb on - strand at 2327.334 kb on - strand at 2327.334 kb on - strand at 2327.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0009 mg/ml remove 2,322,782 - +0.9 2,322,804 + Dsui_2187 0.82 +0.9 2,322,850 - Dsui_2187 0.84 -1.4 2,322,906 - +0.9 2,323,017 + -1.4 2,323,034 + -0.1 2,323,048 + +0.9 2,323,096 + -1.4 2,323,176 - +0.9 2,323,329 + -0.1 2,323,431 + +0.4 2,323,450 - +0.9 2,323,457 + Dsui_2188 0.12 +0.1 2,323,547 + Dsui_2188 0.59 +0.2 2,323,587 + Dsui_2188 0.80 +0.4 2,323,595 + Dsui_2188 0.84 +0.0 2,323,619 - -1.1 2,323,640 + -0.4 2,323,669 + +0.8 2,323,669 - +2.5 2,323,749 + +0.9 2,324,195 - Dsui_2189 0.16 -1.4 2,324,280 - Dsui_2189 0.19 -0.7 2,324,420 + -0.1 2,324,474 - Dsui_2189 0.26 -1.9 2,324,764 - -0.1 2,324,974 - Dsui_2189 0.45 -1.4 2,325,069 + +0.9 2,325,199 + -0.1 2,325,290 + -0.1 2,325,736 - Dsui_2189 0.73 -2.1 2,325,754 - Dsui_2189 0.74 -2.4 2,325,827 - Dsui_2189 0.76 -2.9 2,325,836 - Dsui_2189 0.77 -3.4 2,325,847 - Dsui_2189 0.77 -1.5 2,326,085 - Dsui_2189 0.86 -0.7 2,326,087 - Dsui_2189 0.86 -1.1 2,326,087 - Dsui_2189 0.86 +0.0 2,326,222 - -3.1 2,326,222 - -3.1 2,326,456 + +0.9 2,326,584 + +0.9 2,326,773 - -0.7 2,326,842 - -0.1 2,326,842 - +0.9 2,327,039 - -0.7 2,327,071 + +0.9 2,327,088 + Dsui_2190 0.56 -0.1 2,327,111 + Dsui_2190 0.60 -1.1 2,327,135 - +0.9 2,327,294 + +0.9 2,327,334 - +0.9 2,327,334 - +0.9 2,327,334 - -0.7 2,327,359 - -1.1
Or see this region's nucleotide sequence