Experiment: ALP with Cisplatin 0.0009 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1809 and Dsui_1810 are separated by 15 nucleotides Dsui_1810 and Dsui_1811 overlap by 1 nucleotides Dsui_1811 and Dsui_1812 are separated by 209 nucleotides Dsui_1812 and Dsui_1813 are separated by 3 nucleotides
Dsui_1809: Dsui_1809 - DNA polymerase III, subunit gamma/tau, at 1,906,920 to 1,908,566
_1809
Dsui_1810: Dsui_1810 - DNA-binding protein, YbaB/EbfC family, at 1,908,582 to 1,908,905
_1810
Dsui_1811: Dsui_1811 - recombination protein RecR, at 1,908,905 to 1,909,507
_1811
Dsui_1812: Dsui_1812 - ubiquinol-cytochrome c reductase, iron-sulfur subunit, at 1,909,717 to 1,910,313
_1812
Dsui_1813: Dsui_1813 - cytochrome b subunit of the bc complex, at 1,910,317 to 1,911,621
_1813
Position (kb)
1908
1909
1910 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1908.608 kb on + strand at 1908.645 kb on - strand at 1908.647 kb on + strand, within Dsui_1810 at 1908.657 kb on + strand, within Dsui_1810 at 1908.657 kb on + strand, within Dsui_1810 at 1908.683 kb on - strand, within Dsui_1810 at 1908.841 kb on - strand, within Dsui_1810 at 1908.868 kb on + strand, within Dsui_1810 at 1908.868 kb on + strand, within Dsui_1810 at 1908.876 kb on - strand at 1908.904 kb on - strand at 1908.934 kb on + strand at 1908.942 kb on - strand at 1908.982 kb on + strand, within Dsui_1811 at 1909.089 kb on - strand, within Dsui_1811 at 1909.097 kb on - strand, within Dsui_1811 at 1909.097 kb on - strand, within Dsui_1811 at 1909.192 kb on + strand, within Dsui_1811 at 1909.195 kb on - strand, within Dsui_1811 at 1909.223 kb on + strand, within Dsui_1811 at 1909.474 kb on + strand at 1909.476 kb on - strand at 1909.539 kb on + strand at 1909.541 kb on - strand at 1909.611 kb on + strand at 1909.662 kb on + strand at 1910.296 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0009 mg/ml remove 1,908,608 + -2.4 1,908,645 - -0.3 1,908,647 + Dsui_1810 0.20 -5.5 1,908,657 + Dsui_1810 0.23 -3.4 1,908,657 + Dsui_1810 0.23 -7.0 1,908,683 - Dsui_1810 0.31 -0.9 1,908,841 - Dsui_1810 0.80 +0.7 1,908,868 + Dsui_1810 0.88 -5.1 1,908,868 + Dsui_1810 0.88 -6.3 1,908,876 - -0.3 1,908,904 - -0.9 1,908,934 + -4.5 1,908,942 - -0.9 1,908,982 + Dsui_1811 0.13 -5.6 1,909,089 - Dsui_1811 0.31 -0.9 1,909,097 - Dsui_1811 0.32 -0.3 1,909,097 - Dsui_1811 0.32 -1.3 1,909,192 + Dsui_1811 0.48 -5.3 1,909,195 - Dsui_1811 0.48 +0.7 1,909,223 + Dsui_1811 0.53 -6.1 1,909,474 + -4.6 1,909,476 - -3.1 1,909,539 + +0.4 1,909,541 - -0.8 1,909,611 + -0.5 1,909,662 + -1.0 1,910,296 + -2.7
Or see this region's nucleotide sequence