Experiment: ALP with Cisplatin 0.0009 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1599 and Dsui_1600 overlap by 4 nucleotides Dsui_1600 and Dsui_1601 are separated by 27 nucleotides Dsui_1601 and Dsui_1602 are separated by 142 nucleotides
Dsui_1599: Dsui_1599 - putative enzyme of heme biosynthesis, at 1,679,632 to 1,680,834
_1599
Dsui_1600: Dsui_1600 - putative enzyme of heme biosynthesis, at 1,680,831 to 1,681,916
_1600
Dsui_1601: Dsui_1601 - uroporphyrinogen-III synthase, at 1,681,944 to 1,682,711
_1601
Dsui_1602: Dsui_1602 - porphobilinogen deaminase, at 1,682,854 to 1,683,813
_1602
Position (kb)
1680
1681
1682 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1680.002 kb on + strand, within Dsui_1599 at 1680.010 kb on - strand, within Dsui_1599 at 1680.102 kb on + strand, within Dsui_1599 at 1680.251 kb on + strand, within Dsui_1599 at 1680.366 kb on + strand, within Dsui_1599 at 1680.389 kb on - strand, within Dsui_1599 at 1680.394 kb on + strand, within Dsui_1599 at 1680.584 kb on + strand, within Dsui_1599 at 1680.592 kb on - strand, within Dsui_1599 at 1680.769 kb on - strand at 1680.849 kb on - strand at 1680.851 kb on + strand at 1680.859 kb on - strand at 1680.861 kb on + strand at 1680.868 kb on + strand at 1681.078 kb on - strand, within Dsui_1600 at 1681.090 kb on + strand, within Dsui_1600 at 1681.113 kb on - strand, within Dsui_1600 at 1681.131 kb on + strand at 1681.189 kb on - strand, within Dsui_1600 at 1681.386 kb on - strand, within Dsui_1600 at 1681.471 kb on + strand, within Dsui_1600 at 1681.504 kb on - strand, within Dsui_1600 at 1681.504 kb on - strand, within Dsui_1600 at 1681.513 kb on + strand at 1681.518 kb on + strand at 1681.656 kb on - strand, within Dsui_1600 at 1681.783 kb on - strand, within Dsui_1600 at 1681.890 kb on - strand at 1681.919 kb on - strand at 1681.930 kb on - strand at 1682.738 kb on + strand at 1682.762 kb on + strand at 1682.774 kb on - strand at 1682.779 kb on + strand at 1682.846 kb on - strand at 1682.859 kb on + strand at 1682.865 kb on - strand at 1682.869 kb on + strand at 1682.872 kb on - strand at 1682.872 kb on - strand at 1682.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0009 mg/ml remove 1,680,002 + Dsui_1599 0.31 -0.5 1,680,010 - Dsui_1599 0.31 -4.3 1,680,102 + Dsui_1599 0.39 -1.1 1,680,251 + Dsui_1599 0.51 -0.5 1,680,366 + Dsui_1599 0.61 -1.5 1,680,389 - Dsui_1599 0.63 -4.8 1,680,394 + Dsui_1599 0.63 -1.1 1,680,584 + Dsui_1599 0.79 -1.5 1,680,592 - Dsui_1599 0.80 -4.3 1,680,769 - -4.4 1,680,849 - -4.9 1,680,851 + +0.5 1,680,859 - -2.6 1,680,861 + -1.8 1,680,868 + -0.5 1,681,078 - Dsui_1600 0.23 -3.6 1,681,090 + Dsui_1600 0.24 -0.5 1,681,113 - Dsui_1600 0.26 -3.2 1,681,131 + +0.5 1,681,189 - Dsui_1600 0.33 -5.0 1,681,386 - Dsui_1600 0.51 -2.6 1,681,471 + Dsui_1600 0.59 +0.5 1,681,504 - Dsui_1600 0.62 -3.6 1,681,504 - Dsui_1600 0.62 -3.8 1,681,513 + +0.5 1,681,518 + -0.5 1,681,656 - Dsui_1600 0.76 -4.0 1,681,783 - Dsui_1600 0.88 -4.0 1,681,890 - -4.0 1,681,919 - -2.0 1,681,930 - -3.3 1,682,738 + -0.5 1,682,762 + +0.5 1,682,774 - -1.0 1,682,779 + -1.1 1,682,846 - -0.9 1,682,859 + -2.1 1,682,865 - -3.0 1,682,869 + +0.5 1,682,872 - -0.1 1,682,872 - -0.3 1,682,872 - -0.7
Or see this region's nucleotide sequence