Experiment: ALP with Cisplatin 0.0009 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1416 and Dsui_1417 are separated by 48 nucleotides Dsui_1417 and Dsui_1418 overlap by 1 nucleotides Dsui_1418 and Dsui_1419 are separated by 7 nucleotides Dsui_1419 and Dsui_1420 overlap by 4 nucleotides Dsui_1420 and Dsui_1421 are separated by 39 nucleotides
Dsui_1416: Dsui_1416 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 1,472,448 to 1,473,086
_1416
Dsui_1417: Dsui_1417 - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, at 1,473,135 to 1,473,875
_1417
Dsui_1418: Dsui_1418 - imidazoleglycerol phosphate synthase, cyclase subunit, at 1,473,875 to 1,474,636
_1418
Dsui_1419: Dsui_1419 - phosphoribosyl-AMP cyclohydrolase, at 1,474,644 to 1,475,051
_1419
Dsui_1420: Dsui_1420 - phosphoribosyl-ATP pyrophosphohydrolase, at 1,475,048 to 1,475,371
_1420
Dsui_1421: Dsui_1421 - HIT family hydrolase, diadenosine tetraphosphate hydrolase, at 1,475,411 to 1,475,770
_1421
Position (kb)
1473
1474
1475 Strain fitness (log2 ratio)
-1
0
1 at 1475.406 kb on - strand at 1475.416 kb on - strand at 1475.416 kb on - strand at 1475.463 kb on + strand, within Dsui_1421 at 1475.465 kb on + strand, within Dsui_1421 at 1475.465 kb on + strand, within Dsui_1421 at 1475.465 kb on + strand, within Dsui_1421 at 1475.465 kb on + strand, within Dsui_1421 at 1475.473 kb on - strand, within Dsui_1421 at 1475.473 kb on - strand, within Dsui_1421 at 1475.630 kb on + strand, within Dsui_1421
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0009 mg/ml remove 1,475,406 - +0.5 1,475,416 - +0.5 1,475,416 - +1.5 1,475,463 + Dsui_1421 0.14 -0.3 1,475,465 + Dsui_1421 0.15 -0.8 1,475,465 + Dsui_1421 0.15 +0.3 1,475,465 + Dsui_1421 0.15 +0.5 1,475,465 + Dsui_1421 0.15 -0.4 1,475,473 - Dsui_1421 0.17 -1.1 1,475,473 - Dsui_1421 0.17 +0.5 1,475,630 + Dsui_1421 0.61 +0.5
Or see this region's nucleotide sequence