Experiment: ALP with Cisplatin 0.0009 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0309 and Dsui_0310 are separated by 50 nucleotides Dsui_0310 and Dsui_0311 are separated by 99 nucleotides Dsui_0311 and Dsui_0312 are separated by 14 nucleotides Dsui_0312 and Dsui_0313 are separated by 45 nucleotides
Dsui_0309: Dsui_0309 - DNA/RNA helicase, superfamily II, at 311,154 to 312,485
_0309
Dsui_0310: Dsui_0310 - acyl-CoA hydrolase, at 312,536 to 313,036
_0310
Dsui_0311: Dsui_0311 - ATP-dependent DNA helicase RecG, at 313,136 to 315,211
_0311
Dsui_0312: Dsui_0312 - endoribonuclease L-PSP, putative, at 315,226 to 315,606
_0312
Dsui_0313: Dsui_0313 - putative transcriptional regulator, at 315,652 to 316,005
_0313
Position (kb) 313
314
315
316 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 312.181 kb on + strand, within Dsui_0309 at 312.394 kb on + strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.491 kb on + strand at 312.750 kb on + strand, within Dsui_0310 at 312.805 kb on + strand, within Dsui_0310 at 312.805 kb on + strand at 312.876 kb on - strand, within Dsui_0310 at 312.917 kb on + strand at 312.925 kb on - strand, within Dsui_0310 at 312.925 kb on - strand, within Dsui_0310 at 312.925 kb on - strand, within Dsui_0310 at 312.925 kb on - strand, within Dsui_0310 at 312.947 kb on - strand, within Dsui_0310 at 313.031 kb on + strand at 313.064 kb on + strand at 313.080 kb on - strand at 313.100 kb on + strand at 313.124 kb on - strand at 313.140 kb on + strand at 313.148 kb on - strand at 313.153 kb on + strand at 313.224 kb on + strand at 313.267 kb on + strand at 313.267 kb on + strand at 313.295 kb on + strand at 313.370 kb on - strand, within Dsui_0311 at 313.412 kb on - strand, within Dsui_0311 at 313.544 kb on + strand at 313.643 kb on + strand, within Dsui_0311 at 313.773 kb on - strand, within Dsui_0311 at 314.076 kb on - strand, within Dsui_0311 at 314.106 kb on + strand at 314.110 kb on + strand, within Dsui_0311 at 314.144 kb on - strand, within Dsui_0311 at 314.190 kb on + strand at 314.346 kb on + strand at 314.643 kb on + strand, within Dsui_0311 at 314.659 kb on + strand, within Dsui_0311 at 314.687 kb on + strand at 314.756 kb on + strand at 314.941 kb on - strand, within Dsui_0311 at 315.022 kb on + strand at 315.045 kb on + strand at 315.129 kb on - strand at 315.129 kb on - strand at 315.224 kb on - strand at 315.664 kb on + strand at 315.725 kb on - strand, within Dsui_0313 at 315.727 kb on - strand at 315.741 kb on + strand, within Dsui_0313 at 315.741 kb on + strand at 315.749 kb on - strand, within Dsui_0313 at 315.749 kb on - strand, within Dsui_0313 at 315.749 kb on - strand, within Dsui_0313 at 315.749 kb on - strand, within Dsui_0313 at 315.749 kb on - strand, within Dsui_0313 at 315.756 kb on - strand, within Dsui_0313 at 315.776 kb on + strand, within Dsui_0313 at 315.851 kb on - strand, within Dsui_0313 at 315.967 kb on + strand at 315.999 kb on + strand at 316.089 kb on - strand at 316.091 kb on + strand at 316.109 kb on + strand at 316.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0009 mg/ml remove 312,181 + Dsui_0309 0.77 -2.2 312,394 + -1.2 312,488 - +1.0 312,488 - +0.4 312,491 + +0.4 312,750 + Dsui_0310 0.43 -0.6 312,805 + Dsui_0310 0.54 -1.6 312,805 + +0.4 312,876 - Dsui_0310 0.68 -0.2 312,917 + -1.2 312,925 - Dsui_0310 0.78 -0.3 312,925 - Dsui_0310 0.78 -0.1 312,925 - Dsui_0310 0.78 -0.1 312,925 - Dsui_0310 0.78 -0.9 312,947 - Dsui_0310 0.82 -1.6 313,031 + +0.4 313,064 + +0.4 313,080 - -0.5 313,100 + +0.4 313,124 - -0.6 313,140 + +0.4 313,148 - -0.0 313,153 + -1.2 313,224 + +0.4 313,267 + -2.8 313,267 + -0.6 313,295 + -1.2 313,370 - Dsui_0311 0.11 -2.3 313,412 - Dsui_0311 0.13 -3.2 313,544 + +0.4 313,643 + Dsui_0311 0.24 +0.4 313,773 - Dsui_0311 0.31 -4.1 314,076 - Dsui_0311 0.45 -3.3 314,106 + +0.4 314,110 + Dsui_0311 0.47 +0.4 314,144 - Dsui_0311 0.49 -4.3 314,190 + -1.2 314,346 + -1.6 314,643 + Dsui_0311 0.73 -0.6 314,659 + Dsui_0311 0.73 -2.4 314,687 + +0.4 314,756 + +0.4 314,941 - Dsui_0311 0.87 -6.1 315,022 + +0.4 315,045 + -0.6 315,129 - -5.4 315,129 - -3.9 315,224 - -0.3 315,664 + +0.4 315,725 - Dsui_0313 0.21 -1.6 315,727 - -0.6 315,741 + Dsui_0313 0.25 -0.6 315,741 + +0.4 315,749 - Dsui_0313 0.27 -2.2 315,749 - Dsui_0313 0.27 -3.5 315,749 - Dsui_0313 0.27 +0.4 315,749 - Dsui_0313 0.27 -1.9 315,749 - Dsui_0313 0.27 -0.6 315,756 - Dsui_0313 0.29 -1.6 315,776 + Dsui_0313 0.35 -1.3 315,851 - Dsui_0313 0.56 +0.4 315,967 + +0.4 315,999 + -2.2 316,089 - -1.6 316,091 + +2.2 316,109 + -1.2 316,168 - -0.3
Or see this region's nucleotide sequence