Experiment: ALP with Cisplatin 0.0009 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0006 and Dsui_0007 are separated by 85 nucleotides Dsui_0007 and Dsui_0008 are separated by 312 nucleotides Dsui_0008 and Dsui_0009 are separated by 0 nucleotides Dsui_0009 and Dsui_0010 are separated by 107 nucleotides
Dsui_0006: Dsui_0006 - sulfur oxidation protein SoxZ, at 4,836 to 5,153
_0006
Dsui_0007: Dsui_0007 - cold shock protein, at 5,239 to 5,442
_0007
Dsui_0008: Dsui_0008 - hypothetical protein, at 5,755 to 6,072
_0008
Dsui_0009: Dsui_0009 - ATP-dependent Clp protease ATP-binding subunit clpA, at 6,073 to 8,328
_0009
Dsui_0010: Dsui_0010 - RraA family protein, at 8,436 to 8,927
_0010
Position (kb) 6
7
8
9 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 5.077 kb on - strand at 5.123 kb on - strand at 5.123 kb on - strand at 5.164 kb on + strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.173 kb on - strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.297 kb on - strand, within Dsui_0007 at 5.359 kb on + strand at 5.372 kb on - strand, within Dsui_0007 at 5.396 kb on + strand, within Dsui_0007 at 5.443 kb on - strand at 5.492 kb on - strand at 5.547 kb on - strand at 5.569 kb on + strand at 5.587 kb on - strand at 5.600 kb on - strand at 5.616 kb on + strand at 5.651 kb on + strand at 5.651 kb on + strand at 5.651 kb on + strand at 5.659 kb on - strand at 5.659 kb on - strand at 5.659 kb on - strand at 5.659 kb on - strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.710 kb on + strand at 5.718 kb on + strand at 5.718 kb on + strand at 5.718 kb on - strand at 5.718 kb on - strand at 5.726 kb on - strand at 5.726 kb on - strand at 5.730 kb on + strand at 5.730 kb on + strand at 5.730 kb on + strand at 5.730 kb on + strand at 5.738 kb on - strand at 5.740 kb on + strand at 5.748 kb on - strand at 5.750 kb on + strand at 5.751 kb on + strand at 5.751 kb on + strand at 5.759 kb on - strand at 5.759 kb on - strand at 5.776 kb on + strand at 5.776 kb on + strand at 5.776 kb on + strand at 5.781 kb on + strand at 5.784 kb on - strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.786 kb on + strand at 5.794 kb on - strand at 5.794 kb on - strand, within Dsui_0008 at 5.807 kb on + strand, within Dsui_0008 at 5.807 kb on + strand, within Dsui_0008 at 5.814 kb on + strand, within Dsui_0008 at 5.815 kb on - strand, within Dsui_0008 at 5.827 kb on - strand, within Dsui_0008 at 5.831 kb on + strand, within Dsui_0008 at 5.839 kb on - strand, within Dsui_0008 at 5.866 kb on + strand, within Dsui_0008 at 5.866 kb on + strand, within Dsui_0008 at 5.874 kb on - strand, within Dsui_0008 at 5.936 kb on + strand, within Dsui_0008 at 5.972 kb on + strand, within Dsui_0008 at 5.974 kb on + strand, within Dsui_0008 at 5.975 kb on - strand, within Dsui_0008 at 5.975 kb on - strand, within Dsui_0008 at 5.980 kb on - strand, within Dsui_0008 at 5.985 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 5.996 kb on + strand, within Dsui_0008 at 6.002 kb on + strand, within Dsui_0008 at 6.007 kb on + strand, within Dsui_0008 at 6.043 kb on + strand at 6.053 kb on + strand at 6.269 kb on + strand at 6.277 kb on - strand at 6.277 kb on - strand at 6.318 kb on - strand at 6.422 kb on + strand, within Dsui_0009 at 6.555 kb on + strand, within Dsui_0009 at 6.576 kb on + strand, within Dsui_0009 at 6.612 kb on + strand, within Dsui_0009 at 6.662 kb on + strand, within Dsui_0009 at 6.670 kb on - strand, within Dsui_0009 at 6.724 kb on - strand, within Dsui_0009 at 6.750 kb on - strand, within Dsui_0009 at 6.799 kb on - strand, within Dsui_0009 at 6.852 kb on + strand, within Dsui_0009 at 6.864 kb on + strand, within Dsui_0009 at 6.864 kb on + strand, within Dsui_0009 at 6.876 kb on + strand, within Dsui_0009 at 6.884 kb on - strand, within Dsui_0009 at 6.891 kb on + strand, within Dsui_0009 at 6.973 kb on - strand, within Dsui_0009 at 6.982 kb on - strand, within Dsui_0009 at 6.984 kb on + strand, within Dsui_0009 at 6.984 kb on + strand, within Dsui_0009 at 7.019 kb on + strand, within Dsui_0009 at 7.123 kb on - strand, within Dsui_0009 at 7.127 kb on - strand, within Dsui_0009 at 7.147 kb on + strand, within Dsui_0009 at 7.195 kb on + strand, within Dsui_0009 at 7.383 kb on - strand, within Dsui_0009 at 7.609 kb on + strand, within Dsui_0009 at 7.612 kb on - strand, within Dsui_0009 at 7.617 kb on - strand, within Dsui_0009 at 7.617 kb on - strand, within Dsui_0009 at 7.675 kb on + strand, within Dsui_0009 at 7.713 kb on + strand, within Dsui_0009 at 7.734 kb on + strand, within Dsui_0009 at 7.734 kb on + strand, within Dsui_0009 at 7.742 kb on - strand at 7.979 kb on - strand, within Dsui_0009 at 8.105 kb on - strand at 8.126 kb on + strand at 8.157 kb on + strand at 8.269 kb on - strand at 8.331 kb on - strand at 8.335 kb on - strand at 8.441 kb on - strand at 8.443 kb on + strand at 8.449 kb on + strand at 8.449 kb on + strand at 8.449 kb on + strand at 8.449 kb on + strand at 8.449 kb on + strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.457 kb on - strand at 8.485 kb on + strand at 8.485 kb on + strand at 8.521 kb on - strand, within Dsui_0010 at 8.533 kb on + strand, within Dsui_0010 at 8.562 kb on - strand, within Dsui_0010 at 8.601 kb on - strand, within Dsui_0010 at 8.601 kb on - strand, within Dsui_0010 at 8.606 kb on - strand, within Dsui_0010 at 8.618 kb on + strand, within Dsui_0010 at 8.626 kb on - strand, within Dsui_0010 at 8.626 kb on - strand, within Dsui_0010 at 8.764 kb on + strand, within Dsui_0010 at 8.781 kb on + strand, within Dsui_0010 at 8.894 kb on - strand at 8.991 kb on + strand at 9.010 kb on + strand at 9.070 kb on - strand at 9.089 kb on + strand at 9.103 kb on + strand at 9.279 kb on + strand at 9.279 kb on + strand at 9.281 kb on + strand at 9.287 kb on - strand at 9.291 kb on + strand at 9.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0009 mg/ml remove 5,077 - +0.4 5,123 - -1.2 5,123 - +0.4 5,164 + -0.7 5,172 - +0.4 5,172 - -1.6 5,172 - -0.6 5,173 - -1.6 5,237 + -0.6 5,237 + +0.4 5,237 + +0.4 5,244 - -0.4 5,244 - -1.0 5,244 - -1.1 5,297 - Dsui_0007 0.28 -1.0 5,359 + +0.4 5,372 - Dsui_0007 0.65 -1.7 5,396 + Dsui_0007 0.77 +0.4 5,443 - -2.2 5,492 - -1.1 5,547 - -0.9 5,569 + +0.4 5,587 - -0.9 5,600 - -0.8 5,616 + +0.4 5,651 + +0.4 5,651 + +0.4 5,651 + -2.0 5,659 - -1.2 5,659 - -0.6 5,659 - -3.0 5,659 - +0.4 5,710 + +0.4 5,710 + -1.2 5,710 + +0.4 5,710 + -2.0 5,710 + +0.4 5,718 + -0.6 5,718 + +0.4 5,718 - -0.6 5,718 - -1.6 5,726 - +0.4 5,726 - +0.4 5,730 + -0.6 5,730 + +0.4 5,730 + +1.4 5,730 + -1.6 5,738 - -0.6 5,740 + -0.3 5,748 - +0.4 5,750 + -0.2 5,751 + -0.1 5,751 + -0.5 5,759 - -0.6 5,759 - +0.4 5,776 + +0.1 5,776 + -0.6 5,776 + -1.9 5,781 + -0.4 5,784 - -2.4 5,786 + -1.1 5,786 + -0.2 5,786 + -1.3 5,786 + -0.3 5,786 + -0.7 5,786 + +0.2 5,794 - +0.4 5,794 - Dsui_0008 0.12 +0.4 5,807 + Dsui_0008 0.16 -0.7 5,807 + Dsui_0008 0.16 -1.0 5,814 + Dsui_0008 0.19 -1.0 5,815 - Dsui_0008 0.19 -0.5 5,827 - Dsui_0008 0.23 +0.4 5,831 + Dsui_0008 0.24 -1.1 5,839 - Dsui_0008 0.26 +0.4 5,866 + Dsui_0008 0.35 -0.6 5,866 + Dsui_0008 0.35 -0.4 5,874 - Dsui_0008 0.37 -2.8 5,936 + Dsui_0008 0.57 -0.3 5,972 + Dsui_0008 0.68 -0.4 5,974 + Dsui_0008 0.69 -0.4 5,975 - Dsui_0008 0.69 +0.4 5,975 - Dsui_0008 0.69 +0.4 5,980 - Dsui_0008 0.71 +0.4 5,985 + Dsui_0008 0.72 -0.5 5,996 + Dsui_0008 0.76 -1.1 5,996 + Dsui_0008 0.76 -0.4 5,996 + Dsui_0008 0.76 -0.7 5,996 + Dsui_0008 0.76 -0.4 6,002 + Dsui_0008 0.78 -0.8 6,007 + Dsui_0008 0.79 -0.8 6,043 + -0.4 6,053 + -0.5 6,269 + -2.6 6,277 - -3.5 6,277 - +0.4 6,318 - +0.4 6,422 + Dsui_0009 0.15 -3.4 6,555 + Dsui_0009 0.21 -3.5 6,576 + Dsui_0009 0.22 -4.1 6,612 + Dsui_0009 0.24 -3.8 6,662 + Dsui_0009 0.26 -2.7 6,670 - Dsui_0009 0.26 -2.0 6,724 - Dsui_0009 0.29 -2.0 6,750 - Dsui_0009 0.30 -2.4 6,799 - Dsui_0009 0.32 -2.0 6,852 + Dsui_0009 0.35 -3.9 6,864 + Dsui_0009 0.35 -3.1 6,864 + Dsui_0009 0.35 -3.0 6,876 + Dsui_0009 0.36 -2.4 6,884 - Dsui_0009 0.36 -0.6 6,891 + Dsui_0009 0.36 -3.1 6,973 - Dsui_0009 0.40 +0.4 6,982 - Dsui_0009 0.40 -3.6 6,984 + Dsui_0009 0.40 -4.1 6,984 + Dsui_0009 0.40 -3.6 7,019 + Dsui_0009 0.42 -4.0 7,123 - Dsui_0009 0.47 -1.2 7,127 - Dsui_0009 0.47 -0.6 7,147 + Dsui_0009 0.48 -3.1 7,195 + Dsui_0009 0.50 -4.6 7,383 - Dsui_0009 0.58 -0.6 7,609 + Dsui_0009 0.68 -3.6 7,612 - Dsui_0009 0.68 -2.0 7,617 - Dsui_0009 0.68 -1.2 7,617 - Dsui_0009 0.68 -2.0 7,675 + Dsui_0009 0.71 -3.3 7,713 + Dsui_0009 0.73 -3.8 7,734 + Dsui_0009 0.74 -4.6 7,734 + Dsui_0009 0.74 -4.2 7,742 - +0.4 7,979 - Dsui_0009 0.84 -2.2 8,105 - -2.0 8,126 + -7.1 8,157 + -4.7 8,269 - -1.2 8,331 - -2.8 8,335 - -0.6 8,441 - -2.0 8,443 + +0.2 8,449 + -0.1 8,449 + -0.9 8,449 + -1.9 8,449 + -2.6 8,449 + -0.6 8,457 - -0.6 8,457 - +0.4 8,457 - +0.4 8,457 - -0.6 8,457 - -1.2 8,457 - +0.4 8,457 - +0.0 8,485 + -2.8 8,485 + -2.0 8,521 - Dsui_0010 0.17 -1.2 8,533 + Dsui_0010 0.20 -0.4 8,562 - Dsui_0010 0.26 -0.6 8,601 - Dsui_0010 0.34 -2.0 8,601 - Dsui_0010 0.34 -1.6 8,606 - Dsui_0010 0.35 +0.4 8,618 + Dsui_0010 0.37 -3.0 8,626 - Dsui_0010 0.39 +0.4 8,626 - Dsui_0010 0.39 -0.6 8,764 + Dsui_0010 0.67 -0.9 8,781 + Dsui_0010 0.70 +0.1 8,894 - +0.4 8,991 + -3.2 9,010 + +0.4 9,070 - +0.4 9,089 + -2.8 9,103 + +0.4 9,279 + -2.0 9,279 + +0.4 9,281 + -3.5 9,287 - +0.4 9,291 + -2.2 9,291 + -1.6
Or see this region's nucleotide sequence