Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1532 and BT1533 are separated by 32 nucleotides BT1533 and BT1534 are separated by 37 nucleotides BT1534 and BT1535 are separated by 29 nucleotides
BT1532: BT1532 - ABC transporter permease (NCBI ptt file), at 1,891,100 to 1,892,353
BT1532
BT1533: BT1533 - conserved hypothetical protein (NCBI ptt file), at 1,892,386 to 1,893,675
BT1533
BT1534: BT1534 - hypothetical protein (NCBI ptt file), at 1,893,713 to 1,894,339
BT1534
BT1535: BT1535 - ABC transporter ATP-binding protein (NCBI ptt file), at 1,894,369 to 1,895,034
BT1535
Position (kb)
1892
1893
1894 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1891.507 kb on + strand, within BT1532 at 1891.510 kb on - strand, within BT1532 at 1891.521 kb on - strand, within BT1532 at 1891.531 kb on - strand, within BT1532 at 1891.550 kb on - strand, within BT1532 at 1891.672 kb on + strand, within BT1532 at 1891.711 kb on + strand, within BT1532 at 1891.726 kb on - strand, within BT1532 at 1891.746 kb on + strand, within BT1532 at 1891.828 kb on + strand, within BT1532 at 1891.840 kb on + strand, within BT1532 at 1891.858 kb on + strand, within BT1532 at 1892.031 kb on - strand, within BT1532 at 1892.070 kb on - strand, within BT1532 at 1892.152 kb on - strand, within BT1532 at 1892.165 kb on - strand, within BT1532 at 1892.194 kb on - strand, within BT1532 at 1892.194 kb on - strand, within BT1532 at 1892.233 kb on + strand at 1892.247 kb on + strand at 1892.248 kb on - strand at 1892.263 kb on + strand at 1892.263 kb on + strand at 1892.264 kb on - strand at 1892.266 kb on - strand at 1892.266 kb on - strand at 1892.359 kb on + strand at 1892.412 kb on + strand at 1892.521 kb on - strand, within BT1533 at 1892.556 kb on + strand, within BT1533 at 1892.604 kb on - strand, within BT1533 at 1892.649 kb on + strand, within BT1533 at 1892.650 kb on - strand, within BT1533 at 1892.773 kb on + strand at 1892.798 kb on - strand, within BT1533 at 1892.802 kb on + strand, within BT1533 at 1892.805 kb on - strand, within BT1533 at 1892.922 kb on + strand, within BT1533 at 1892.925 kb on - strand, within BT1533 at 1892.925 kb on - strand, within BT1533 at 1893.080 kb on + strand, within BT1533 at 1893.129 kb on + strand, within BT1533 at 1893.196 kb on + strand, within BT1533 at 1893.197 kb on - strand, within BT1533 at 1893.197 kb on - strand, within BT1533 at 1893.208 kb on + strand, within BT1533 at 1893.246 kb on + strand, within BT1533 at 1893.280 kb on - strand, within BT1533 at 1893.324 kb on + strand, within BT1533 at 1893.324 kb on + strand, within BT1533 at 1893.381 kb on + strand, within BT1533 at 1893.381 kb on + strand, within BT1533 at 1893.382 kb on - strand, within BT1533 at 1893.432 kb on + strand, within BT1533 at 1893.507 kb on + strand, within BT1533 at 1893.574 kb on - strand at 1893.601 kb on + strand at 1893.602 kb on - strand at 1893.602 kb on - strand at 1893.602 kb on - strand at 1893.602 kb on - strand at 1893.602 kb on - strand at 1893.628 kb on - strand at 1893.628 kb on - strand at 1893.678 kb on + strand at 1893.679 kb on - strand at 1893.760 kb on + strand at 1893.859 kb on + strand, within BT1534 at 1894.098 kb on + strand, within BT1534 at 1894.180 kb on - strand, within BT1534 at 1894.244 kb on + strand, within BT1534 at 1894.292 kb on + strand at 1894.292 kb on + strand at 1894.292 kb on + strand at 1894.301 kb on + strand at 1894.319 kb on + strand at 1894.370 kb on + strand at 1894.370 kb on + strand at 1894.401 kb on + strand at 1894.471 kb on - strand, within BT1535 at 1894.525 kb on - strand, within BT1535 at 1894.580 kb on - strand, within BT1535 at 1894.632 kb on + strand, within BT1535
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 1,891,507 + BT1532 0.32 -1.2 1,891,510 - BT1532 0.33 -1.2 1,891,521 - BT1532 0.34 -0.7 1,891,531 - BT1532 0.34 -2.0 1,891,550 - BT1532 0.36 -1.7 1,891,672 + BT1532 0.46 -1.4 1,891,711 + BT1532 0.49 +0.3 1,891,726 - BT1532 0.50 +0.6 1,891,746 + BT1532 0.52 -0.7 1,891,828 + BT1532 0.58 +1.6 1,891,840 + BT1532 0.59 -0.2 1,891,858 + BT1532 0.60 -0.0 1,892,031 - BT1532 0.74 +0.2 1,892,070 - BT1532 0.77 +0.4 1,892,152 - BT1532 0.84 -1.0 1,892,165 - BT1532 0.85 -0.4 1,892,194 - BT1532 0.87 -0.0 1,892,194 - BT1532 0.87 -1.2 1,892,233 + +1.0 1,892,247 + +1.0 1,892,248 - -0.4 1,892,263 + +0.4 1,892,263 + -1.4 1,892,264 - -0.7 1,892,266 - +0.8 1,892,266 - -2.1 1,892,359 + -0.0 1,892,412 + -1.2 1,892,521 - BT1533 0.10 +1.3 1,892,556 + BT1533 0.13 +0.3 1,892,604 - BT1533 0.17 -0.7 1,892,649 + BT1533 0.20 -0.5 1,892,650 - BT1533 0.20 -0.4 1,892,773 + +0.6 1,892,798 - BT1533 0.32 -1.0 1,892,802 + BT1533 0.32 -0.3 1,892,805 - BT1533 0.32 -2.7 1,892,922 + BT1533 0.42 +0.8 1,892,925 - BT1533 0.42 -1.2 1,892,925 - BT1533 0.42 +1.6 1,893,080 + BT1533 0.54 +0.8 1,893,129 + BT1533 0.58 +2.6 1,893,196 + BT1533 0.63 -1.6 1,893,197 - BT1533 0.63 -0.7 1,893,197 - BT1533 0.63 +1.6 1,893,208 + BT1533 0.64 +0.6 1,893,246 + BT1533 0.67 -1.7 1,893,280 - BT1533 0.69 -0.9 1,893,324 + BT1533 0.73 +0.2 1,893,324 + BT1533 0.73 +0.8 1,893,381 + BT1533 0.77 -2.2 1,893,381 + BT1533 0.77 -2.0 1,893,382 - BT1533 0.77 +0.1 1,893,432 + BT1533 0.81 +0.5 1,893,507 + BT1533 0.87 -0.3 1,893,574 - -0.9 1,893,601 + -1.9 1,893,602 - +2.4 1,893,602 - -0.4 1,893,602 - +1.3 1,893,602 - +0.6 1,893,602 - -1.0 1,893,628 - -0.7 1,893,628 - +0.6 1,893,678 + -0.6 1,893,679 - -0.1 1,893,760 + -0.4 1,893,859 + BT1534 0.23 -2.1 1,894,098 + BT1534 0.61 -0.9 1,894,180 - BT1534 0.74 -1.3 1,894,244 + BT1534 0.85 -1.4 1,894,292 + -0.6 1,894,292 + +0.0 1,894,292 + +0.0 1,894,301 + +0.3 1,894,319 + -0.4 1,894,370 + -0.6 1,894,370 + -2.1 1,894,401 + -0.6 1,894,471 - BT1535 0.15 -0.9 1,894,525 - BT1535 0.23 -0.0 1,894,580 - BT1535 0.32 -0.6 1,894,632 + BT1535 0.39 +0.6
Or see this region's nucleotide sequence