Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5959 and Pf1N1B4_5960 are separated by 7 nucleotides Pf1N1B4_5960 and Pf1N1B4_5961 are separated by 63 nucleotides Pf1N1B4_5961 and Pf1N1B4_5962 overlap by 42 nucleotides Pf1N1B4_5962 and Pf1N1B4_5963 are separated by 233 nucleotides
Pf1N1B4_5959 - Probable signal peptide protein, at 408,684 to 408,947
_5959
Pf1N1B4_5960 - Cytochrome b, at 408,955 to 409,482
_5960
Pf1N1B4_5961 - Major facilitator superfamily transporter, at 409,546 to 411,222
_5961
Pf1N1B4_5962 - hypothetical protein, at 411,181 to 411,342
_5962
Pf1N1B4_5963 - Predicted regulator PutR for proline utilization, GntR family, at 411,576 to 412,247
_5963
Position (kb)
409
410
411
412 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 408.667 kb on + strand at 408.667 kb on + strand at 408.667 kb on + strand at 408.667 kb on + strand at 408.668 kb on - strand at 408.668 kb on - strand at 408.690 kb on - strand at 408.817 kb on - strand, within Pf1N1B4_5959 at 408.855 kb on + strand, within Pf1N1B4_5959 at 408.865 kb on - strand, within Pf1N1B4_5959 at 408.865 kb on - strand, within Pf1N1B4_5959 at 408.865 kb on - strand, within Pf1N1B4_5959 at 408.900 kb on + strand, within Pf1N1B4_5959 at 408.900 kb on + strand, within Pf1N1B4_5959 at 408.900 kb on + strand, within Pf1N1B4_5959 at 408.901 kb on - strand at 408.901 kb on - strand, within Pf1N1B4_5959 at 408.945 kb on + strand at 408.992 kb on + strand at 408.992 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 408.996 kb on + strand at 409.084 kb on + strand, within Pf1N1B4_5960 at 409.085 kb on - strand, within Pf1N1B4_5960 at 409.091 kb on + strand, within Pf1N1B4_5960 at 409.092 kb on - strand, within Pf1N1B4_5960 at 409.321 kb on + strand, within Pf1N1B4_5960 at 409.321 kb on + strand, within Pf1N1B4_5960 at 409.322 kb on - strand, within Pf1N1B4_5960 at 409.322 kb on - strand, within Pf1N1B4_5960 at 409.547 kb on + strand at 409.547 kb on + strand at 409.547 kb on + strand at 409.547 kb on + strand at 409.547 kb on + strand at 409.547 kb on + strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.548 kb on - strand at 409.646 kb on + strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.647 kb on - strand at 409.797 kb on - strand, within Pf1N1B4_5961 at 409.797 kb on - strand, within Pf1N1B4_5961 at 409.811 kb on + strand, within Pf1N1B4_5961 at 409.811 kb on + strand, within Pf1N1B4_5961 at 409.811 kb on + strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 409.812 kb on - strand, within Pf1N1B4_5961 at 410.043 kb on - strand, within Pf1N1B4_5961 at 410.043 kb on - strand, within Pf1N1B4_5961 at 410.043 kb on - strand, within Pf1N1B4_5961 at 410.043 kb on - strand, within Pf1N1B4_5961 at 410.043 kb on - strand, within Pf1N1B4_5961 at 410.201 kb on + strand, within Pf1N1B4_5961 at 410.232 kb on - strand, within Pf1N1B4_5961 at 410.248 kb on + strand, within Pf1N1B4_5961 at 410.249 kb on - strand, within Pf1N1B4_5961 at 410.405 kb on + strand, within Pf1N1B4_5961 at 410.420 kb on + strand, within Pf1N1B4_5961 at 410.470 kb on + strand, within Pf1N1B4_5961 at 410.582 kb on + strand at 410.777 kb on + strand, within Pf1N1B4_5961 at 410.777 kb on + strand, within Pf1N1B4_5961 at 410.778 kb on - strand at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.938 kb on - strand at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.996 kb on - strand, within Pf1N1B4_5961 at 410.996 kb on - strand, within Pf1N1B4_5961 at 411.205 kb on - strand at 411.248 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.268 kb on - strand, within Pf1N1B4_5962 at 411.394 kb on + strand at 411.435 kb on - strand at 411.490 kb on - strand at 411.490 kb on - strand at 411.614 kb on + strand at 411.614 kb on + strand at 411.877 kb on + strand, within Pf1N1B4_5963 at 411.878 kb on - strand, within Pf1N1B4_5963 at 412.062 kb on - strand, within Pf1N1B4_5963
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 408,667 + +0.9 408,667 + -0.3 408,667 + +0.6 408,667 + +0.8 408,668 - -0.0 408,668 - +0.3 408,690 - +0.1 408,817 - Pf1N1B4_5959 0.50 +0.9 408,855 + Pf1N1B4_5959 0.65 +0.8 408,865 - Pf1N1B4_5959 0.69 +0.2 408,865 - Pf1N1B4_5959 0.69 +0.5 408,865 - Pf1N1B4_5959 0.69 -0.0 408,900 + Pf1N1B4_5959 0.82 +0.2 408,900 + Pf1N1B4_5959 0.82 -0.0 408,900 + Pf1N1B4_5959 0.82 -0.2 408,901 - +1.2 408,901 - Pf1N1B4_5959 0.82 +1.0 408,945 + +1.9 408,992 + -0.1 408,992 + -0.0 408,996 + +1.7 408,996 + +2.1 408,996 + +0.4 408,996 + +0.5 408,996 + -0.2 408,996 + -0.4 408,996 + -0.2 409,084 + Pf1N1B4_5960 0.24 -0.2 409,085 - Pf1N1B4_5960 0.25 -0.5 409,091 + Pf1N1B4_5960 0.26 -0.8 409,092 - Pf1N1B4_5960 0.26 -0.6 409,321 + Pf1N1B4_5960 0.69 -1.2 409,321 + Pf1N1B4_5960 0.69 -0.1 409,322 - Pf1N1B4_5960 0.70 -1.3 409,322 - Pf1N1B4_5960 0.70 -0.5 409,547 + -0.3 409,547 + +0.6 409,547 + +0.1 409,547 + +0.8 409,547 + -0.3 409,547 + -0.2 409,548 - -0.8 409,548 - +0.6 409,548 - +1.2 409,548 - +0.4 409,548 - -0.1 409,548 - -0.2 409,548 - +1.1 409,548 - -0.0 409,548 - -0.6 409,548 - -0.6 409,646 + +0.7 409,647 - -0.8 409,647 - -0.3 409,647 - +0.2 409,647 - +0.7 409,647 - -1.3 409,647 - -0.8 409,797 - Pf1N1B4_5961 0.15 -1.9 409,797 - Pf1N1B4_5961 0.15 -0.1 409,811 + Pf1N1B4_5961 0.16 -0.3 409,811 + Pf1N1B4_5961 0.16 -0.5 409,811 + Pf1N1B4_5961 0.16 -0.4 409,812 - Pf1N1B4_5961 0.16 +0.7 409,812 - Pf1N1B4_5961 0.16 +0.8 409,812 - Pf1N1B4_5961 0.16 -1.1 409,812 - Pf1N1B4_5961 0.16 -1.1 409,812 - Pf1N1B4_5961 0.16 +0.3 409,812 - Pf1N1B4_5961 0.16 +1.4 409,812 - Pf1N1B4_5961 0.16 +0.5 410,043 - Pf1N1B4_5961 0.30 -0.6 410,043 - Pf1N1B4_5961 0.30 +0.0 410,043 - Pf1N1B4_5961 0.30 +0.0 410,043 - Pf1N1B4_5961 0.30 +0.0 410,043 - Pf1N1B4_5961 0.30 +0.6 410,201 + Pf1N1B4_5961 0.39 -0.2 410,232 - Pf1N1B4_5961 0.41 -0.8 410,248 + Pf1N1B4_5961 0.42 +0.2 410,249 - Pf1N1B4_5961 0.42 +0.7 410,405 + Pf1N1B4_5961 0.51 -0.5 410,420 + Pf1N1B4_5961 0.52 -0.4 410,470 + Pf1N1B4_5961 0.55 +0.7 410,582 + +0.3 410,777 + Pf1N1B4_5961 0.73 +0.9 410,777 + Pf1N1B4_5961 0.73 -1.6 410,778 - -0.1 410,778 - Pf1N1B4_5961 0.73 -0.8 410,778 - Pf1N1B4_5961 0.73 +0.0 410,778 - Pf1N1B4_5961 0.73 +0.6 410,778 - Pf1N1B4_5961 0.73 -1.4 410,937 + Pf1N1B4_5961 0.83 +0.2 410,937 + Pf1N1B4_5961 0.83 +1.0 410,937 + Pf1N1B4_5961 0.83 +0.1 410,938 - +3.0 410,938 - Pf1N1B4_5961 0.83 -0.8 410,938 - -0.5 410,938 - Pf1N1B4_5961 0.83 +0.4 410,938 - Pf1N1B4_5961 0.83 -1.6 410,938 - Pf1N1B4_5961 0.83 -1.6 410,996 - Pf1N1B4_5961 0.86 +0.8 410,996 - Pf1N1B4_5961 0.86 -0.9 411,205 - -0.2 411,248 - Pf1N1B4_5962 0.41 +0.1 411,250 - Pf1N1B4_5962 0.43 -0.2 411,250 - Pf1N1B4_5962 0.43 -0.4 411,250 - Pf1N1B4_5962 0.43 -0.1 411,250 - Pf1N1B4_5962 0.43 +0.9 411,268 - Pf1N1B4_5962 0.54 -1.0 411,394 + +0.3 411,435 - +0.1 411,490 - -0.0 411,490 - -0.4 411,614 + +0.8 411,614 + -0.4 411,877 + Pf1N1B4_5963 0.45 -1.5 411,878 - Pf1N1B4_5963 0.45 -0.0 412,062 - Pf1N1B4_5963 0.72 +0.1
Or see this region's nucleotide sequence