Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5827

Experiment: LB 10C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5824 and Pf1N1B4_5825 overlap by 4 nucleotidesPf1N1B4_5825 and Pf1N1B4_5826 overlap by 4 nucleotidesPf1N1B4_5826 and Pf1N1B4_5827 are separated by 8 nucleotidesPf1N1B4_5827 and Pf1N1B4_5828 are separated by 95 nucleotides Pf1N1B4_5824 - Na(+) H(+) antiporter subunit D, at 259,307 to 260,983 _5824 Pf1N1B4_5825 - Na(+) H(+) antiporter subunit E, at 260,980 to 261,468 _5825 Pf1N1B4_5826 - Na(+) H(+) antiporter subunit F, at 261,465 to 261,743 _5826 Pf1N1B4_5827 - Na(+) H(+) antiporter subunit G, at 261,752 to 262,102 _5827 Pf1N1B4_5828 - Mobile element protein, at 262,198 to 263,499 _5828 Position (kb) 261 262 263Strain fitness (log2 ratio) -2 -1 0 1at 260.906 kb on + strandat 260.906 kb on + strandat 260.906 kb on + strandat 260.906 kb on + strandat 260.907 kb on - strandat 260.907 kb on - strandat 260.907 kb on - strandat 261.068 kb on + strand, within Pf1N1B4_5825at 261.595 kb on - strand, within Pf1N1B4_5826at 261.595 kb on - strand, within Pf1N1B4_5826at 261.595 kb on - strand, within Pf1N1B4_5826at 261.808 kb on + strand, within Pf1N1B4_5827at 261.923 kb on + strand, within Pf1N1B4_5827at 262.075 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 10C
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260,906 + +1.0
260,906 + -0.3
260,906 + +0.9
260,906 + -0.3
260,907 - +1.4
260,907 - -0.5
260,907 - -2.0
261,068 + Pf1N1B4_5825 0.18 +0.4
261,595 - Pf1N1B4_5826 0.47 -0.6
261,595 - Pf1N1B4_5826 0.47 -1.3
261,595 - Pf1N1B4_5826 0.47 +0.3
261,808 + Pf1N1B4_5827 0.16 -1.0
261,923 + Pf1N1B4_5827 0.49 -0.5
262,075 + -0.6

Or see this region's nucleotide sequence