Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5240 and Pf1N1B4_5241 are separated by 198 nucleotides Pf1N1B4_5241 and Pf1N1B4_5242 overlap by 8 nucleotides Pf1N1B4_5242 and Pf1N1B4_5243 are separated by 71 nucleotides Pf1N1B4_5243 and Pf1N1B4_5244 are separated by 85 nucleotides Pf1N1B4_5244 and Pf1N1B4_5245 are separated by 42 nucleotides
Pf1N1B4_5240 - hypothetical protein, at 753 to 872
_5240
Pf1N1B4_5241 - hypothetical protein, at 1,071 to 1,271
_5241
Pf1N1B4_5242 - hypothetical protein, at 1,264 to 1,524
_5242
Pf1N1B4_5243 - hypothetical protein, at 1,596 to 1,763
_5243
Pf1N1B4_5244 - hypothetical protein, at 1,849 to 2,097
_5244
Pf1N1B4_5245 - hypothetical protein, at 2,140 to 2,784
_5245
Position (kb)
1
2 Strain fitness (log2 ratio)
-2
-1
0
1 at 0.597 kb on - strand at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.943 kb on + strand at 0.943 kb on + strand at 0.992 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.075 kb on - strand at 1.075 kb on - strand at 1.092 kb on - strand, within Pf1N1B4_5241 at 1.265 kb on + strand at 1.265 kb on + strand at 1.337 kb on - strand, within Pf1N1B4_5242 at 1.369 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.501 kb on + strand at 1.501 kb on + strand at 1.664 kb on + strand, within Pf1N1B4_5243 at 1.664 kb on + strand, within Pf1N1B4_5243 at 1.665 kb on - strand, within Pf1N1B4_5243 at 1.689 kb on + strand at 1.786 kb on + strand at 1.787 kb on - strand at 1.953 kb on + strand, within Pf1N1B4_5244 at 1.954 kb on - strand, within Pf1N1B4_5244 at 2.026 kb on + strand, within Pf1N1B4_5244 at 2.042 kb on + strand, within Pf1N1B4_5244 at 2.096 kb on - strand at 2.096 kb on - strand at 2.239 kb on - strand, within Pf1N1B4_5245 at 2.240 kb on + strand at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.241 kb on - strand, within Pf1N1B4_5245 at 2.265 kb on - strand, within Pf1N1B4_5245 at 2.265 kb on - strand, within Pf1N1B4_5245 at 2.324 kb on - strand, within Pf1N1B4_5245 at 2.346 kb on - strand, within Pf1N1B4_5245 at 2.370 kb on + strand, within Pf1N1B4_5245 at 2.588 kb on + strand, within Pf1N1B4_5245 at 2.588 kb on + strand at 2.589 kb on - strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.648 kb on + strand, within Pf1N1B4_5245 at 2.649 kb on - strand, within Pf1N1B4_5245 at 2.649 kb on - strand, within Pf1N1B4_5245 at 2.678 kb on + strand, within Pf1N1B4_5245 at 2.679 kb on - strand, within Pf1N1B4_5245 at 2.679 kb on - strand, within Pf1N1B4_5245
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 597 - +0.6 773 - Pf1N1B4_5240 0.17 +1.0 773 - Pf1N1B4_5240 0.17 -0.5 773 - Pf1N1B4_5240 0.17 +1.2 943 + +0.8 943 + +0.3 992 + -0.1 1,074 + -0.0 1,074 + -1.0 1,074 + +0.5 1,074 + +1.2 1,074 + -1.7 1,075 - -1.6 1,075 - -0.1 1,092 - Pf1N1B4_5241 0.10 -0.9 1,265 + -0.3 1,265 + -0.3 1,337 - Pf1N1B4_5242 0.28 +0.6 1,369 + Pf1N1B4_5242 0.40 +0.4 1,407 + Pf1N1B4_5242 0.55 +0.5 1,407 + Pf1N1B4_5242 0.55 -0.3 1,501 + -0.0 1,501 + -0.3 1,664 + Pf1N1B4_5243 0.40 -1.8 1,664 + Pf1N1B4_5243 0.40 -0.6 1,665 - Pf1N1B4_5243 0.41 -0.8 1,689 + +0.9 1,786 + +0.3 1,787 - -0.5 1,953 + Pf1N1B4_5244 0.42 -0.3 1,954 - Pf1N1B4_5244 0.42 +0.5 2,026 + Pf1N1B4_5244 0.71 -0.6 2,042 + Pf1N1B4_5244 0.78 -0.1 2,096 - -0.6 2,096 - -2.3 2,239 - Pf1N1B4_5245 0.15 -0.0 2,240 + +1.6 2,241 - Pf1N1B4_5245 0.16 -0.7 2,241 - Pf1N1B4_5245 0.16 -0.6 2,241 - Pf1N1B4_5245 0.16 -0.6 2,241 - Pf1N1B4_5245 0.16 +1.1 2,241 - Pf1N1B4_5245 0.16 -0.1 2,241 - Pf1N1B4_5245 0.16 -0.5 2,241 - Pf1N1B4_5245 0.16 +0.1 2,265 - Pf1N1B4_5245 0.19 +1.1 2,265 - Pf1N1B4_5245 0.19 +0.6 2,324 - Pf1N1B4_5245 0.29 +0.2 2,346 - Pf1N1B4_5245 0.32 -0.8 2,370 + Pf1N1B4_5245 0.36 -0.4 2,588 + Pf1N1B4_5245 0.69 -0.2 2,588 + -1.4 2,589 - Pf1N1B4_5245 0.70 -0.8 2,648 + Pf1N1B4_5245 0.79 -1.0 2,648 + Pf1N1B4_5245 0.79 +0.4 2,648 + +0.6 2,648 + Pf1N1B4_5245 0.79 -0.1 2,648 + Pf1N1B4_5245 0.79 +0.7 2,648 + Pf1N1B4_5245 0.79 -0.7 2,648 + Pf1N1B4_5245 0.79 -1.4 2,649 - Pf1N1B4_5245 0.79 -0.8 2,649 - Pf1N1B4_5245 0.79 -0.2 2,678 + Pf1N1B4_5245 0.83 -0.2 2,679 - Pf1N1B4_5245 0.84 -0.4 2,679 - Pf1N1B4_5245 0.84 -2.2
Or see this region's nucleotide sequence