Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5239 and Pf1N1B4_5240 are separated by 173 nucleotides Pf1N1B4_5240 and Pf1N1B4_5241 are separated by 198 nucleotides Pf1N1B4_5241 and Pf1N1B4_5242 overlap by 8 nucleotides
Pf1N1B4_5239 - hypothetical protein, at 184 to 579
_5239
Pf1N1B4_5240 - hypothetical protein, at 753 to 872
_5240
Pf1N1B4_5241 - hypothetical protein, at 1,071 to 1,271
_5241
Pf1N1B4_5242 - hypothetical protein, at 1,264 to 1,524
_5242
Position (kb) 0
1 Strain fitness (log2 ratio) -2
-1
0
1 at 0.011 kb on + strand at 0.011 kb on + strand at 0.011 kb on + strand at 0.040 kb on + strand at 0.040 kb on + strand at 0.040 kb on + strand at 0.041 kb on - strand at 0.124 kb on + strand at 0.124 kb on + strand at 0.125 kb on - strand at 0.125 kb on - strand at 0.125 kb on - strand at 0.146 kb on - strand at 0.186 kb on - strand at 0.284 kb on + strand, within Pf1N1B4_5239 at 0.285 kb on - strand, within Pf1N1B4_5239 at 0.463 kb on - strand, within Pf1N1B4_5239 at 0.494 kb on + strand, within Pf1N1B4_5239 at 0.494 kb on + strand at 0.495 kb on - strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.497 kb on - strand, within Pf1N1B4_5239 at 0.597 kb on - strand at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.943 kb on + strand at 0.943 kb on + strand at 0.992 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.075 kb on - strand at 1.075 kb on - strand at 1.092 kb on - strand, within Pf1N1B4_5241 at 1.265 kb on + strand at 1.265 kb on + strand at 1.337 kb on - strand, within Pf1N1B4_5242 at 1.369 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.501 kb on + strand at 1.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 11 + -0.2 11 + -0.3 11 + +0.0 40 + +0.2 40 + -0.3 40 + +0.3 41 - -0.3 124 + +0.6 124 + +0.4 125 - -0.0 125 - -0.6 125 - -1.0 146 - +0.5 186 - +1.6 284 + Pf1N1B4_5239 0.25 -0.6 285 - Pf1N1B4_5239 0.26 +0.2 463 - Pf1N1B4_5239 0.70 -0.0 494 + Pf1N1B4_5239 0.78 -2.1 494 + +0.4 495 - Pf1N1B4_5239 0.79 -0.6 496 + Pf1N1B4_5239 0.79 +0.1 496 + Pf1N1B4_5239 0.79 -1.1 496 + Pf1N1B4_5239 0.79 -0.3 496 + Pf1N1B4_5239 0.79 -0.1 497 - Pf1N1B4_5239 0.79 -0.5 597 - +0.6 773 - Pf1N1B4_5240 0.17 +1.0 773 - Pf1N1B4_5240 0.17 -0.5 773 - Pf1N1B4_5240 0.17 +1.2 943 + +0.8 943 + +0.3 992 + -0.1 1,074 + -0.0 1,074 + -1.0 1,074 + +0.5 1,074 + +1.2 1,074 + -1.7 1,075 - -1.6 1,075 - -0.1 1,092 - Pf1N1B4_5241 0.10 -0.9 1,265 + -0.3 1,265 + -0.3 1,337 - Pf1N1B4_5242 0.28 +0.6 1,369 + Pf1N1B4_5242 0.40 +0.4 1,407 + Pf1N1B4_5242 0.55 +0.5 1,407 + Pf1N1B4_5242 0.55 -0.3 1,501 + -0.0 1,501 + -0.3
Or see this region's nucleotide sequence