Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4930 and Pf1N1B4_4931 are separated by 252 nucleotides Pf1N1B4_4931 and Pf1N1B4_4932 are separated by 40 nucleotides
Pf1N1B4_4930 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,463,792 to 5,464,949
_4930
Pf1N1B4_4931 - Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20); Succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.79), at 5,465,202 to 5,466,659
_4931
Pf1N1B4_4932 - Tartrate dehydrogenase (EC 1.1.1.93) / Tartrate decarboxylase (EC 4.1.1.73) / D-malic enzyme (EC 1.1.1.83), at 5,466,700 to 5,467,779
_4932
Position (kb) 5465
5466
5467 Strain fitness (log2 ratio) -1
0
1 at 5464.327 kb on - strand, within Pf1N1B4_4930 at 5464.831 kb on + strand at 5464.831 kb on + strand, within Pf1N1B4_4930 at 5464.831 kb on + strand, within Pf1N1B4_4930 at 5464.832 kb on - strand, within Pf1N1B4_4930 at 5465.170 kb on - strand at 5465.250 kb on + strand at 5465.251 kb on - strand at 5465.251 kb on - strand at 5465.251 kb on - strand at 5466.114 kb on + strand, within Pf1N1B4_4931 at 5466.115 kb on - strand, within Pf1N1B4_4931 at 5466.457 kb on - strand at 5466.460 kb on - strand, within Pf1N1B4_4931 at 5466.460 kb on - strand, within Pf1N1B4_4931 at 5466.606 kb on + strand at 5466.622 kb on - strand at 5466.646 kb on + strand at 5466.647 kb on - strand at 5466.647 kb on - strand at 5466.824 kb on + strand, within Pf1N1B4_4932 at 5466.847 kb on + strand at 5467.006 kb on + strand, within Pf1N1B4_4932 at 5467.117 kb on + strand, within Pf1N1B4_4932 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.568 kb on - strand, within Pf1N1B4_4932
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 5,464,327 - Pf1N1B4_4930 0.46 +0.6 5,464,831 + +0.4 5,464,831 + Pf1N1B4_4930 0.90 -0.1 5,464,831 + Pf1N1B4_4930 0.90 +0.6 5,464,832 - Pf1N1B4_4930 0.90 -0.3 5,465,170 - -0.8 5,465,250 + +1.0 5,465,251 - -0.5 5,465,251 - +1.0 5,465,251 - -0.2 5,466,114 + Pf1N1B4_4931 0.63 -0.6 5,466,115 - Pf1N1B4_4931 0.63 -0.6 5,466,457 - -0.1 5,466,460 - Pf1N1B4_4931 0.86 +0.3 5,466,460 - Pf1N1B4_4931 0.86 -1.2 5,466,606 + +0.2 5,466,622 - -0.2 5,466,646 + -0.4 5,466,647 - +0.1 5,466,647 - -0.4 5,466,824 + Pf1N1B4_4932 0.11 +1.2 5,466,847 + -0.0 5,467,006 + Pf1N1B4_4932 0.28 -0.0 5,467,117 + Pf1N1B4_4932 0.39 -0.8 5,467,567 + Pf1N1B4_4932 0.80 -0.9 5,467,567 + Pf1N1B4_4932 0.80 -0.8 5,467,568 - Pf1N1B4_4932 0.80 -0.0
Or see this region's nucleotide sequence