Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4886 and Pf1N1B4_4887 are separated by 158 nucleotides Pf1N1B4_4887 and Pf1N1B4_4888 are separated by 330 nucleotides
Pf1N1B4_4886 - Phosphoglucomutase (EC 5.4.2.2), at 5,405,519 to 5,407,165
_4886
Pf1N1B4_4887 - Pirin, at 5,407,324 to 5,408,019
_4887
Pf1N1B4_4888 - UvrD/REP helicase family protein, at 5,408,350 to 5,410,773
_4888
Position (kb)
5407
5408
5409 Strain fitness (log2 ratio)
-2
-1
0
1 at 5406.494 kb on + strand, within Pf1N1B4_4886 at 5406.494 kb on + strand, within Pf1N1B4_4886 at 5406.494 kb on + strand, within Pf1N1B4_4886 at 5406.494 kb on + strand, within Pf1N1B4_4886 at 5406.495 kb on - strand, within Pf1N1B4_4886 at 5406.495 kb on - strand, within Pf1N1B4_4886 at 5407.067 kb on + strand at 5407.067 kb on + strand at 5407.067 kb on + strand at 5407.067 kb on + strand at 5407.068 kb on - strand at 5407.068 kb on - strand at 5407.068 kb on - strand at 5407.076 kb on + strand at 5407.077 kb on - strand at 5407.077 kb on - strand at 5407.077 kb on - strand at 5407.265 kb on + strand at 5407.800 kb on + strand, within Pf1N1B4_4887 at 5407.801 kb on - strand, within Pf1N1B4_4887 at 5407.820 kb on + strand, within Pf1N1B4_4887 at 5407.821 kb on - strand, within Pf1N1B4_4887 at 5407.821 kb on - strand at 5407.821 kb on - strand, within Pf1N1B4_4887 at 5408.101 kb on + strand at 5408.102 kb on - strand at 5408.483 kb on - strand at 5408.489 kb on - strand at 5408.491 kb on - strand at 5408.797 kb on + strand, within Pf1N1B4_4888 at 5408.868 kb on + strand, within Pf1N1B4_4888
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 5,406,494 + Pf1N1B4_4886 0.59 +0.5 5,406,494 + Pf1N1B4_4886 0.59 +0.6 5,406,494 + Pf1N1B4_4886 0.59 +1.0 5,406,494 + Pf1N1B4_4886 0.59 -0.3 5,406,495 - Pf1N1B4_4886 0.59 -1.8 5,406,495 - Pf1N1B4_4886 0.59 -0.5 5,407,067 + +0.2 5,407,067 + -0.0 5,407,067 + -0.9 5,407,067 + +0.8 5,407,068 - -0.9 5,407,068 - +0.6 5,407,068 - +1.6 5,407,076 + +0.2 5,407,077 - +0.8 5,407,077 - +0.1 5,407,077 - +1.1 5,407,265 + -1.1 5,407,800 + Pf1N1B4_4887 0.68 -0.8 5,407,801 - Pf1N1B4_4887 0.69 -0.6 5,407,820 + Pf1N1B4_4887 0.71 -0.2 5,407,821 - Pf1N1B4_4887 0.71 -0.5 5,407,821 - +0.3 5,407,821 - Pf1N1B4_4887 0.71 +0.8 5,408,101 + +1.2 5,408,102 - +1.6 5,408,483 - -0.6 5,408,489 - +0.4 5,408,491 - +0.6 5,408,797 + Pf1N1B4_4888 0.18 +0.1 5,408,868 + Pf1N1B4_4888 0.21 -0.6
Or see this region's nucleotide sequence