Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4780 and Pf1N1B4_4781 are separated by 56 nucleotides Pf1N1B4_4781 and Pf1N1B4_4782 are separated by 160 nucleotides Pf1N1B4_4782 and Pf1N1B4_4783 are separated by 157 nucleotides
Pf1N1B4_4780 - COGs COG3558, at 5,281,745 to 5,282,221
_4780
Pf1N1B4_4781 - Transcriptional regulator, TetR family, at 5,282,278 to 5,282,898
_4781
Pf1N1B4_4782 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 5,283,059 to 5,284,402
_4782
Pf1N1B4_4783 - Transcriptional regulator, AraC family, at 5,284,560 to 5,285,576
_4783
Position (kb) 5283
5284
5285 Strain fitness (log2 ratio) -2
-1
0
1 at 5282.216 kb on - strand at 5282.222 kb on - strand at 5282.222 kb on - strand at 5282.224 kb on - strand at 5282.243 kb on - strand at 5282.267 kb on + strand at 5282.710 kb on - strand, within Pf1N1B4_4781 at 5282.991 kb on - strand at 5283.017 kb on + strand at 5283.017 kb on + strand at 5283.018 kb on - strand at 5283.059 kb on - strand at 5283.059 kb on - strand at 5283.091 kb on + strand at 5283.092 kb on - strand at 5283.200 kb on + strand at 5283.200 kb on + strand, within Pf1N1B4_4782 at 5283.200 kb on + strand at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.201 kb on - strand at 5283.201 kb on - strand, within Pf1N1B4_4782 at 5283.216 kb on - strand, within Pf1N1B4_4782 at 5283.722 kb on + strand, within Pf1N1B4_4782 at 5283.723 kb on - strand, within Pf1N1B4_4782 at 5284.010 kb on + strand, within Pf1N1B4_4782 at 5284.112 kb on + strand, within Pf1N1B4_4782 at 5284.113 kb on - strand, within Pf1N1B4_4782 at 5284.113 kb on - strand, within Pf1N1B4_4782 at 5284.475 kb on + strand at 5284.519 kb on + strand at 5284.519 kb on + strand at 5284.520 kb on - strand at 5284.714 kb on - strand, within Pf1N1B4_4783 at 5284.974 kb on + strand, within Pf1N1B4_4783 at 5284.974 kb on + strand, within Pf1N1B4_4783 at 5284.975 kb on - strand at 5284.984 kb on - strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783 at 5285.385 kb on + strand, within Pf1N1B4_4783
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 5,282,216 - -0.2 5,282,222 - -0.2 5,282,222 - +0.4 5,282,224 - +0.2 5,282,243 - -0.7 5,282,267 + -0.9 5,282,710 - Pf1N1B4_4781 0.70 -0.6 5,282,991 - -0.4 5,283,017 + +0.6 5,283,017 + +0.6 5,283,018 - -1.9 5,283,059 - -1.7 5,283,059 - -0.2 5,283,091 + -0.1 5,283,092 - -0.9 5,283,200 + +0.6 5,283,200 + Pf1N1B4_4782 0.10 -1.3 5,283,200 + +1.6 5,283,201 - Pf1N1B4_4782 0.11 -2.7 5,283,201 - Pf1N1B4_4782 0.11 -0.7 5,283,201 - Pf1N1B4_4782 0.11 +1.1 5,283,201 - Pf1N1B4_4782 0.11 -0.7 5,283,201 - Pf1N1B4_4782 0.11 -0.2 5,283,201 - -0.4 5,283,201 - Pf1N1B4_4782 0.11 -1.1 5,283,216 - Pf1N1B4_4782 0.12 -1.6 5,283,722 + Pf1N1B4_4782 0.49 -1.9 5,283,723 - Pf1N1B4_4782 0.49 +0.2 5,284,010 + Pf1N1B4_4782 0.71 -0.8 5,284,112 + Pf1N1B4_4782 0.78 -0.8 5,284,113 - Pf1N1B4_4782 0.78 -0.3 5,284,113 - Pf1N1B4_4782 0.78 -0.7 5,284,475 + +1.0 5,284,519 + -0.2 5,284,519 + -1.0 5,284,520 - +0.9 5,284,714 - Pf1N1B4_4783 0.15 -0.0 5,284,974 + Pf1N1B4_4783 0.41 +0.1 5,284,974 + Pf1N1B4_4783 0.41 +0.4 5,284,975 - -1.4 5,284,984 - Pf1N1B4_4783 0.42 -1.7 5,285,385 + Pf1N1B4_4783 0.81 -0.8 5,285,385 + Pf1N1B4_4783 0.81 +0.2 5,285,385 + Pf1N1B4_4783 0.81 -0.3
Or see this region's nucleotide sequence