Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_211 and Pf1N1B4_212 overlap by 25 nucleotides Pf1N1B4_212 and Pf1N1B4_213 are separated by 122 nucleotides Pf1N1B4_213 and Pf1N1B4_214 are separated by 202 nucleotides
Pf1N1B4_211 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 259,661 to 260,527
_211
Pf1N1B4_212 - Thiol:disulfide interchange protein DsbG precursor, at 260,503 to 261,270
_212
Pf1N1B4_213 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 261,393 to 262,205
_213
Pf1N1B4_214 - Pyoverdine chromophore precursor synthetase PvdL, at 262,408 to 267,885
_214
Position (kb) 261
262
263 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 260.675 kb on - strand, within Pf1N1B4_212 at 260.698 kb on + strand, within Pf1N1B4_212 at 260.698 kb on + strand, within Pf1N1B4_212 at 260.699 kb on - strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.792 kb on - strand, within Pf1N1B4_212 at 260.792 kb on - strand, within Pf1N1B4_212 at 260.878 kb on + strand, within Pf1N1B4_212 at 260.879 kb on - strand, within Pf1N1B4_212 at 260.906 kb on - strand, within Pf1N1B4_212 at 261.187 kb on + strand, within Pf1N1B4_212 at 261.188 kb on - strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.192 kb on - strand, within Pf1N1B4_212 at 261.192 kb on - strand at 261.192 kb on - strand, within Pf1N1B4_212 at 261.192 kb on - strand, within Pf1N1B4_212 at 261.268 kb on + strand at 261.268 kb on + strand at 261.268 kb on + strand at 261.268 kb on + strand at 261.269 kb on - strand at 261.272 kb on + strand at 261.273 kb on - strand at 261.342 kb on + strand at 261.343 kb on - strand at 261.385 kb on - strand at 261.394 kb on + strand at 261.462 kb on - strand at 261.462 kb on - strand at 261.487 kb on + strand, within Pf1N1B4_213 at 261.488 kb on - strand, within Pf1N1B4_213 at 261.623 kb on + strand, within Pf1N1B4_213 at 261.749 kb on + strand, within Pf1N1B4_213 at 261.750 kb on - strand, within Pf1N1B4_213 at 261.750 kb on - strand, within Pf1N1B4_213 at 261.811 kb on + strand, within Pf1N1B4_213 at 261.811 kb on + strand, within Pf1N1B4_213 at 261.811 kb on + strand, within Pf1N1B4_213 at 261.812 kb on - strand, within Pf1N1B4_213 at 261.812 kb on - strand, within Pf1N1B4_213 at 261.812 kb on - strand at 261.812 kb on - strand, within Pf1N1B4_213 at 261.814 kb on + strand, within Pf1N1B4_213 at 261.847 kb on + strand, within Pf1N1B4_213 at 261.847 kb on + strand, within Pf1N1B4_213 at 261.848 kb on - strand, within Pf1N1B4_213 at 262.106 kb on + strand at 262.106 kb on + strand, within Pf1N1B4_213 at 262.123 kb on + strand, within Pf1N1B4_213 at 262.123 kb on + strand, within Pf1N1B4_213 at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.168 kb on + strand at 262.168 kb on + strand at 262.168 kb on + strand at 262.169 kb on - strand at 262.169 kb on - strand at 262.226 kb on + strand at 262.227 kb on - strand at 262.227 kb on - strand at 262.227 kb on - strand at 262.230 kb on - strand at 262.289 kb on + strand at 262.289 kb on + strand at 262.289 kb on + strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.409 kb on + strand at 262.410 kb on - strand at 262.455 kb on + strand at 262.494 kb on - strand at 262.668 kb on + strand at 262.823 kb on + strand at 262.823 kb on + strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.908 kb on - strand at 262.908 kb on - strand at 262.908 kb on - strand at 263.102 kb on + strand, within Pf1N1B4_214 at 263.175 kb on - strand, within Pf1N1B4_214 at 263.175 kb on - strand, within Pf1N1B4_214 at 263.175 kb on - strand, within Pf1N1B4_214
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 260,675 - Pf1N1B4_212 0.22 -1.1 260,698 + Pf1N1B4_212 0.25 +1.2 260,698 + Pf1N1B4_212 0.25 -0.6 260,699 - Pf1N1B4_212 0.26 +0.1 260,723 + Pf1N1B4_212 0.29 -0.6 260,723 + Pf1N1B4_212 0.29 +0.2 260,723 + Pf1N1B4_212 0.29 +0.8 260,723 + Pf1N1B4_212 0.29 +0.7 260,723 + Pf1N1B4_212 0.29 +0.1 260,724 - Pf1N1B4_212 0.29 -0.5 260,724 - Pf1N1B4_212 0.29 +0.7 260,724 - Pf1N1B4_212 0.29 -0.5 260,724 - Pf1N1B4_212 0.29 +0.2 260,724 - Pf1N1B4_212 0.29 -0.5 260,792 - Pf1N1B4_212 0.38 -1.7 260,792 - Pf1N1B4_212 0.38 -0.3 260,878 + Pf1N1B4_212 0.49 -0.4 260,879 - Pf1N1B4_212 0.49 +0.1 260,906 - Pf1N1B4_212 0.52 +0.8 261,187 + Pf1N1B4_212 0.89 -1.3 261,188 - Pf1N1B4_212 0.89 -0.1 261,191 + Pf1N1B4_212 0.90 +0.8 261,191 + Pf1N1B4_212 0.90 -0.5 261,191 + Pf1N1B4_212 0.90 +0.2 261,191 + Pf1N1B4_212 0.90 -0.2 261,191 + Pf1N1B4_212 0.90 -0.1 261,191 + Pf1N1B4_212 0.90 +0.8 261,191 + Pf1N1B4_212 0.90 -0.6 261,192 - Pf1N1B4_212 0.90 +0.0 261,192 - -0.4 261,192 - Pf1N1B4_212 0.90 +0.2 261,192 - Pf1N1B4_212 0.90 +0.1 261,268 + -0.6 261,268 + -0.6 261,268 + -1.0 261,268 + -0.2 261,269 - -0.1 261,272 + -0.2 261,273 - -1.0 261,342 + +0.3 261,343 - -0.4 261,385 - -0.8 261,394 + -0.3 261,462 - -2.6 261,462 - +0.6 261,487 + Pf1N1B4_213 0.12 -0.1 261,488 - Pf1N1B4_213 0.12 -1.0 261,623 + Pf1N1B4_213 0.28 -1.6 261,749 + Pf1N1B4_213 0.44 -1.7 261,750 - Pf1N1B4_213 0.44 -1.5 261,750 - Pf1N1B4_213 0.44 +0.4 261,811 + Pf1N1B4_213 0.51 -3.2 261,811 + Pf1N1B4_213 0.51 -0.5 261,811 + Pf1N1B4_213 0.51 -0.5 261,812 - Pf1N1B4_213 0.52 -1.3 261,812 - Pf1N1B4_213 0.52 -2.6 261,812 - +1.1 261,812 - Pf1N1B4_213 0.52 -0.3 261,814 + Pf1N1B4_213 0.52 -0.9 261,847 + Pf1N1B4_213 0.56 -0.9 261,847 + Pf1N1B4_213 0.56 -0.3 261,848 - Pf1N1B4_213 0.56 +1.0 262,106 + -1.3 262,106 + Pf1N1B4_213 0.88 -0.5 262,123 + Pf1N1B4_213 0.90 -0.1 262,123 + Pf1N1B4_213 0.90 -0.9 262,164 + +0.6 262,164 + -0.8 262,164 + -0.9 262,164 + +0.7 262,164 + -0.2 262,164 + +1.0 262,165 - +0.0 262,165 - -2.1 262,165 - -0.4 262,165 - -0.7 262,165 - -1.8 262,165 - +0.4 262,168 + -0.2 262,168 + -0.9 262,168 + +0.6 262,169 - -0.3 262,169 - -1.4 262,226 + -0.0 262,227 - +0.0 262,227 - +1.2 262,227 - +1.1 262,230 - +0.4 262,289 + -1.0 262,289 + +0.2 262,289 + +0.6 262,290 - -0.2 262,290 - -0.5 262,290 - -0.2 262,290 - -0.2 262,290 - -1.0 262,290 - +2.3 262,409 + +0.2 262,410 - +1.2 262,455 + +0.8 262,494 - -0.3 262,668 + -3.3 262,823 + +0.4 262,823 + +0.2 262,824 - -0.1 262,824 - +0.3 262,824 - -0.2 262,824 - +0.0 262,824 - +0.1 262,824 - -0.8 262,908 - -0.1 262,908 - +0.7 262,908 - +0.1 263,102 + Pf1N1B4_214 0.13 -0.4 263,175 - Pf1N1B4_214 0.14 -0.3 263,175 - Pf1N1B4_214 0.14 +0.1 263,175 - Pf1N1B4_214 0.14 -0.5
Or see this region's nucleotide sequence