Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08150 and MMP_RS08155 are separated by 102 nucleotides MMP_RS08155 and MMP_RS08160 are separated by 21 nucleotides MMP_RS08160 and MMP_RS08165 are separated by 32 nucleotides MMP_RS08165 and MMP_RS08170 are separated by 143 nucleotides
MMP_RS08150: MMP_RS08150 - agmatinase, at 1,534,843 to 1,535,691
_RS08150
MMP_RS08155: MMP_RS08155 - hypothetical protein, at 1,535,794 to 1,536,036
_RS08155
MMP_RS08160: MMP_RS08160 - EamA family transporter, at 1,536,058 to 1,536,471
_RS08160
MMP_RS08165: MMP_RS08165 - phosphoglycerate dehydrogenase, at 1,536,504 to 1,538,075
_RS08165
MMP_RS08170: MMP_RS08170 - glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit, at 1,538,219 to 1,539,319
_RS08170
Position (kb)
1536
1537
1538
1539 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1535.542 kb on + strand at 1535.544 kb on + strand at 1535.634 kb on - strand at 1535.677 kb on + strand at 1535.693 kb on + strand at 1535.750 kb on - strand at 1535.762 kb on - strand at 1535.763 kb on + strand at 1535.763 kb on + strand at 1535.810 kb on - strand at 1535.832 kb on + strand, within MMP_RS08155 at 1535.856 kb on - strand, within MMP_RS08155 at 1535.856 kb on - strand, within MMP_RS08155 at 1535.860 kb on - strand, within MMP_RS08155 at 1535.860 kb on - strand, within MMP_RS08155 at 1535.860 kb on - strand, within MMP_RS08155 at 1535.860 kb on - strand, within MMP_RS08155 at 1535.861 kb on + strand, within MMP_RS08155 at 1535.861 kb on + strand, within MMP_RS08155 at 1535.861 kb on + strand, within MMP_RS08155 at 1535.861 kb on + strand, within MMP_RS08155 at 1535.865 kb on + strand, within MMP_RS08155 at 1535.901 kb on + strand, within MMP_RS08155 at 1536.197 kb on + strand, within MMP_RS08160 at 1536.332 kb on + strand, within MMP_RS08160 at 1536.335 kb on + strand, within MMP_RS08160 at 1536.343 kb on - strand, within MMP_RS08160 at 1536.364 kb on - strand, within MMP_RS08160 at 1536.421 kb on + strand, within MMP_RS08160 at 1536.425 kb on + strand, within MMP_RS08160 at 1536.426 kb on - strand, within MMP_RS08160 at 1536.432 kb on + strand at 1536.506 kb on + strand at 1536.506 kb on + strand at 1536.532 kb on + strand at 1536.591 kb on - strand at 1536.651 kb on - strand at 1537.063 kb on + strand, within MMP_RS08165 at 1537.357 kb on - strand, within MMP_RS08165 at 1537.378 kb on + strand, within MMP_RS08165 at 1538.061 kb on + strand at 1538.077 kb on - strand at 1538.154 kb on + strand at 1538.154 kb on + strand at 1538.162 kb on - strand at 1538.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,535,542 + -1.1 1,535,544 + -1.7 1,535,634 - -0.9 1,535,677 + -0.9 1,535,693 + +0.1 1,535,750 - +0.7 1,535,762 - +1.0 1,535,763 + +0.9 1,535,763 + +0.7 1,535,810 - +0.4 1,535,832 + MMP_RS08155 0.16 +0.9 1,535,856 - MMP_RS08155 0.26 +0.8 1,535,856 - MMP_RS08155 0.26 +0.8 1,535,860 - MMP_RS08155 0.27 +0.4 1,535,860 - MMP_RS08155 0.27 +0.8 1,535,860 - MMP_RS08155 0.27 +0.2 1,535,860 - MMP_RS08155 0.27 +1.3 1,535,861 + MMP_RS08155 0.28 +0.8 1,535,861 + MMP_RS08155 0.28 +0.5 1,535,861 + MMP_RS08155 0.28 +0.2 1,535,861 + MMP_RS08155 0.28 +0.1 1,535,865 + MMP_RS08155 0.29 +0.4 1,535,901 + MMP_RS08155 0.44 +0.9 1,536,197 + MMP_RS08160 0.34 +0.3 1,536,332 + MMP_RS08160 0.66 +0.2 1,536,335 + MMP_RS08160 0.67 +0.5 1,536,343 - MMP_RS08160 0.69 -0.2 1,536,364 - MMP_RS08160 0.74 +0.6 1,536,421 + MMP_RS08160 0.88 -0.4 1,536,425 + MMP_RS08160 0.89 +0.7 1,536,426 - MMP_RS08160 0.89 +0.4 1,536,432 + +0.3 1,536,506 + +0.3 1,536,506 + +0.5 1,536,532 + -0.1 1,536,591 - -0.7 1,536,651 - -0.3 1,537,063 + MMP_RS08165 0.36 -0.4 1,537,357 - MMP_RS08165 0.54 +2.0 1,537,378 + MMP_RS08165 0.56 -1.7 1,538,061 + -1.0 1,538,077 - -3.2 1,538,154 + +0.2 1,538,154 + +0.6 1,538,162 - +0.2 1,538,709 + -0.0
Or see this region's nucleotide sequence