Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06540 and MMP_RS06545 are separated by 10 nucleotides MMP_RS06545 and MMP_RS06550 are separated by 83 nucleotides MMP_RS06550 and MMP_RS06555 are separated by 51 nucleotides MMP_RS06555 and MMP_RS06560 are separated by 262 nucleotides
MMP_RS06540: MMP_RS06540 - 3-methyl-2-oxobutanoate dehydrogenase subunit VorB, at 1,256,753 to 1,257,808
_RS06540
MMP_RS06545: MMP_RS06545 - ferredoxin family protein, at 1,257,819 to 1,258,058
_RS06545
MMP_RS06550: MMP_RS06550 - AMP-binding protein, at 1,258,142 to 1,259,815
_RS06550
MMP_RS06555: MMP_RS06555 - cupin domain-containing protein, at 1,259,867 to 1,260,421
_RS06555
MMP_RS06560: MMP_RS06560 - hypothetical protein, at 1,260,684 to 1,261,130
_RS06560
Position (kb)
1258
1259
1260 Strain fitness (log2 ratio)
-2
-1
0
1 at 1257.685 kb on + strand, within MMP_RS06540 at 1257.705 kb on + strand at 1257.738 kb on - strand at 1257.756 kb on - strand at 1257.756 kb on - strand at 1257.757 kb on + strand at 1257.810 kb on + strand at 1257.812 kb on + strand at 1257.812 kb on + strand at 1257.853 kb on + strand, within MMP_RS06545 at 1257.877 kb on - strand, within MMP_RS06545 at 1257.922 kb on + strand, within MMP_RS06545 at 1257.929 kb on - strand, within MMP_RS06545 at 1257.963 kb on - strand, within MMP_RS06545 at 1258.027 kb on + strand, within MMP_RS06545 at 1258.109 kb on + strand at 1258.130 kb on - strand at 1258.139 kb on - strand at 1258.143 kb on - strand at 1258.320 kb on + strand, within MMP_RS06550 at 1258.406 kb on + strand, within MMP_RS06550 at 1258.670 kb on - strand, within MMP_RS06550 at 1258.676 kb on - strand, within MMP_RS06550 at 1258.733 kb on + strand, within MMP_RS06550 at 1258.733 kb on + strand, within MMP_RS06550 at 1258.908 kb on - strand, within MMP_RS06550 at 1258.937 kb on + strand, within MMP_RS06550 at 1259.003 kb on - strand, within MMP_RS06550 at 1259.449 kb on - strand, within MMP_RS06550 at 1259.611 kb on - strand, within MMP_RS06550 at 1259.660 kb on + strand at 1259.660 kb on + strand at 1259.846 kb on - strand at 1259.860 kb on - strand at 1259.863 kb on + strand at 1259.864 kb on - strand at 1259.869 kb on + strand at 1259.907 kb on + strand at 1259.998 kb on - strand, within MMP_RS06555 at 1260.141 kb on - strand, within MMP_RS06555 at 1260.422 kb on + strand at 1260.422 kb on + strand at 1260.451 kb on + strand at 1260.456 kb on + strand at 1260.460 kb on - strand at 1260.463 kb on + strand at 1260.501 kb on - strand at 1260.501 kb on - strand at 1260.502 kb on + strand at 1260.522 kb on + strand at 1260.535 kb on + strand at 1260.535 kb on + strand at 1260.535 kb on + strand at 1260.549 kb on - strand at 1260.550 kb on + strand at 1260.551 kb on - strand at 1260.552 kb on + strand at 1260.552 kb on + strand at 1260.552 kb on + strand at 1260.564 kb on + strand at 1260.611 kb on - strand at 1260.612 kb on + strand at 1260.612 kb on + strand at 1260.612 kb on + strand at 1260.645 kb on - strand at 1260.648 kb on + strand at 1260.733 kb on - strand, within MMP_RS06560 at 1260.733 kb on - strand, within MMP_RS06560 at 1260.734 kb on + strand, within MMP_RS06560 at 1260.768 kb on - strand, within MMP_RS06560 at 1260.768 kb on - strand, within MMP_RS06560 at 1260.768 kb on - strand, within MMP_RS06560 at 1260.769 kb on + strand, within MMP_RS06560 at 1260.779 kb on + strand, within MMP_RS06560 at 1260.779 kb on + strand, within MMP_RS06560
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,257,685 + MMP_RS06540 0.88 +0.4 1,257,705 + +0.3 1,257,738 - +0.1 1,257,756 - +1.3 1,257,756 - -0.4 1,257,757 + +0.3 1,257,810 + +0.7 1,257,812 + +1.0 1,257,812 + +0.7 1,257,853 + MMP_RS06545 0.14 -0.2 1,257,877 - MMP_RS06545 0.24 +0.9 1,257,922 + MMP_RS06545 0.43 +0.4 1,257,929 - MMP_RS06545 0.46 +0.2 1,257,963 - MMP_RS06545 0.60 +0.1 1,258,027 + MMP_RS06545 0.87 +0.1 1,258,109 + +0.2 1,258,130 - +0.0 1,258,139 - +0.6 1,258,143 - +0.3 1,258,320 + MMP_RS06550 0.11 +0.3 1,258,406 + MMP_RS06550 0.16 +0.8 1,258,670 - MMP_RS06550 0.32 +0.1 1,258,676 - MMP_RS06550 0.32 +0.4 1,258,733 + MMP_RS06550 0.35 +0.5 1,258,733 + MMP_RS06550 0.35 -1.0 1,258,908 - MMP_RS06550 0.46 +0.6 1,258,937 + MMP_RS06550 0.47 +0.6 1,259,003 - MMP_RS06550 0.51 +0.3 1,259,449 - MMP_RS06550 0.78 +0.2 1,259,611 - MMP_RS06550 0.88 +0.3 1,259,660 + -0.0 1,259,660 + +0.5 1,259,846 - +0.6 1,259,860 - +0.6 1,259,863 + +0.2 1,259,864 - +0.2 1,259,869 + +0.2 1,259,907 + +0.5 1,259,998 - MMP_RS06555 0.24 +0.5 1,260,141 - MMP_RS06555 0.49 +0.3 1,260,422 + -0.2 1,260,422 + -0.2 1,260,451 + +0.5 1,260,456 + +0.0 1,260,460 - +0.5 1,260,463 + +0.6 1,260,501 - -0.2 1,260,501 - +0.4 1,260,502 + -0.2 1,260,522 + +0.5 1,260,535 + +0.1 1,260,535 + -0.5 1,260,535 + -0.6 1,260,549 - +0.1 1,260,550 + -0.3 1,260,551 - +0.5 1,260,552 + +0.8 1,260,552 + +0.3 1,260,552 + -0.2 1,260,564 + +0.3 1,260,611 - +0.0 1,260,612 + +0.1 1,260,612 + +0.3 1,260,612 + +0.0 1,260,645 - -0.1 1,260,648 + -2.4 1,260,733 - MMP_RS06560 0.11 +0.1 1,260,733 - MMP_RS06560 0.11 +0.1 1,260,734 + MMP_RS06560 0.11 +0.1 1,260,768 - MMP_RS06560 0.19 +0.1 1,260,768 - MMP_RS06560 0.19 -1.1 1,260,768 - MMP_RS06560 0.19 +0.1 1,260,769 + MMP_RS06560 0.19 +0.5 1,260,779 + MMP_RS06560 0.21 -0.2 1,260,779 + MMP_RS06560 0.21 +0.2
Or see this region's nucleotide sequence