Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06110 and MMP_RS06115 are separated by 30 nucleotides MMP_RS06115 and MMP_RS06120 are separated by 71 nucleotides MMP_RS06120 and MMP_RS06125 are separated by 142 nucleotides MMP_RS06125 and MMP_RS06130 are separated by 68 nucleotides
MMP_RS06110: MMP_RS06110 - ATP-dependent protease LonB, at 1,168,587 to 1,170,665
_RS06110
MMP_RS06115: MMP_RS06115 - class II aldolase/adducin family protein, at 1,170,696 to 1,171,238
_RS06115
MMP_RS06120: MMP_RS06120 - UPF0147 family protein, at 1,171,310 to 1,171,585
_RS06120
MMP_RS06125: MMP_RS06125 - ribose-5-phosphate isomerase RpiA, at 1,171,728 to 1,172,447
_RS06125
MMP_RS06130: MMP_RS06130 - peptidylprolyl isomerase, at 1,172,516 to 1,172,968
_RS06130
Position (kb)
1171
1172 Strain fitness (log2 ratio)
-2
-1
0
1 at 1170.662 kb on + strand at 1170.669 kb on - strand at 1170.672 kb on - strand at 1170.713 kb on - strand at 1170.735 kb on - strand at 1170.773 kb on + strand, within MMP_RS06115 at 1170.797 kb on + strand, within MMP_RS06115 at 1170.798 kb on - strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.799 kb on + strand, within MMP_RS06115 at 1170.800 kb on - strand, within MMP_RS06115 at 1170.801 kb on + strand, within MMP_RS06115 at 1170.801 kb on + strand, within MMP_RS06115 at 1170.801 kb on + strand, within MMP_RS06115 at 1170.803 kb on - strand, within MMP_RS06115 at 1170.846 kb on + strand, within MMP_RS06115 at 1170.846 kb on + strand, within MMP_RS06115 at 1170.878 kb on + strand, within MMP_RS06115 at 1170.878 kb on + strand, within MMP_RS06115 at 1170.891 kb on + strand, within MMP_RS06115 at 1170.950 kb on + strand, within MMP_RS06115 at 1171.051 kb on + strand, within MMP_RS06115 at 1171.051 kb on + strand, within MMP_RS06115 at 1171.173 kb on - strand, within MMP_RS06115 at 1171.174 kb on + strand, within MMP_RS06115 at 1171.211 kb on + strand at 1171.215 kb on - strand at 1171.216 kb on + strand at 1171.220 kb on - strand at 1171.220 kb on - strand at 1171.221 kb on + strand at 1171.221 kb on + strand at 1171.232 kb on + strand at 1171.242 kb on + strand at 1171.255 kb on + strand at 1171.307 kb on - strand at 1171.337 kb on - strand at 1171.338 kb on + strand, within MMP_RS06120 at 1171.338 kb on + strand, within MMP_RS06120 at 1171.375 kb on - strand, within MMP_RS06120 at 1171.541 kb on + strand, within MMP_RS06120 at 1171.647 kb on - strand at 1171.654 kb on - strand at 1171.654 kb on - strand at 1171.685 kb on + strand at 1171.685 kb on + strand at 1171.710 kb on - strand at 1171.740 kb on - strand at 1171.867 kb on + strand at 1171.928 kb on - strand at 1171.993 kb on + strand at 1172.077 kb on - strand at 1172.083 kb on + strand at 1172.121 kb on - strand at 1172.164 kb on + strand at 1172.220 kb on + strand at 1172.249 kb on - strand at 1172.249 kb on - strand at 1172.250 kb on + strand at 1172.264 kb on - strand at 1172.265 kb on + strand at 1172.436 kb on + strand at 1172.495 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,170,662 + +0.3 1,170,669 - +0.2 1,170,672 - +0.4 1,170,713 - +0.3 1,170,735 - +0.1 1,170,773 + MMP_RS06115 0.14 -0.0 1,170,797 + MMP_RS06115 0.19 -0.2 1,170,798 - MMP_RS06115 0.19 +0.2 1,170,799 + MMP_RS06115 0.19 -0.4 1,170,799 + MMP_RS06115 0.19 +0.1 1,170,799 + MMP_RS06115 0.19 +0.1 1,170,799 + MMP_RS06115 0.19 +0.3 1,170,799 + MMP_RS06115 0.19 +0.3 1,170,799 + MMP_RS06115 0.19 -0.3 1,170,799 + MMP_RS06115 0.19 -0.2 1,170,800 - MMP_RS06115 0.19 +0.1 1,170,801 + MMP_RS06115 0.19 +0.3 1,170,801 + MMP_RS06115 0.19 -0.7 1,170,801 + MMP_RS06115 0.19 +0.4 1,170,803 - MMP_RS06115 0.20 -0.1 1,170,846 + MMP_RS06115 0.28 +0.1 1,170,846 + MMP_RS06115 0.28 +0.0 1,170,878 + MMP_RS06115 0.34 -0.3 1,170,878 + MMP_RS06115 0.34 -0.6 1,170,891 + MMP_RS06115 0.36 -0.4 1,170,950 + MMP_RS06115 0.47 -0.3 1,171,051 + MMP_RS06115 0.65 +0.2 1,171,051 + MMP_RS06115 0.65 +0.3 1,171,173 - MMP_RS06115 0.88 -0.9 1,171,174 + MMP_RS06115 0.88 +0.5 1,171,211 + +0.6 1,171,215 - +0.2 1,171,216 + -0.1 1,171,220 - +0.3 1,171,220 - +0.0 1,171,221 + -0.2 1,171,221 + +0.0 1,171,232 + +0.2 1,171,242 + -0.2 1,171,255 + -0.3 1,171,307 - +0.7 1,171,337 - -0.7 1,171,338 + MMP_RS06120 0.10 -1.6 1,171,338 + MMP_RS06120 0.10 -1.3 1,171,375 - MMP_RS06120 0.24 -1.3 1,171,541 + MMP_RS06120 0.84 -1.3 1,171,647 - -2.2 1,171,654 - -0.1 1,171,654 - -0.2 1,171,685 + +0.2 1,171,685 + +0.2 1,171,710 - -0.2 1,171,740 - +0.6 1,171,867 + -1.4 1,171,928 - -0.5 1,171,993 + -1.5 1,172,077 - -1.8 1,172,083 + +0.1 1,172,121 - -0.8 1,172,164 + -1.2 1,172,220 + -2.0 1,172,249 - -0.8 1,172,249 - -1.1 1,172,250 + -0.8 1,172,264 - -2.1 1,172,265 + -0.8 1,172,436 + -2.3 1,172,495 + -1.0
Or see this region's nucleotide sequence