Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03415 and MMP_RS03420 are separated by 13 nucleotides MMP_RS03420 and MMP_RS03425 are separated by 506 nucleotides MMP_RS03425 and MMP_RS03430 are separated by 21 nucleotides
MMP_RS03415: MMP_RS03415 - acetolactate synthase large subunit, at 641,377 to 643,140
_RS03415
MMP_RS03420: MMP_RS03420 - acetolactate synthase small subunit, at 643,154 to 643,663
_RS03420
MMP_RS03425: MMP_RS03425 - hypothetical protein, at 644,170 to 644,622
_RS03425
MMP_RS03430: MMP_RS03430 - bile acid:sodium symporter family protein, at 644,644 to 645,609
_RS03430
Position (kb)
643
644 Strain fitness (log2 ratio)
-2
-1
0
1 at 642.510 kb on - strand at 642.942 kb on - strand at 643.031 kb on - strand at 643.031 kb on - strand at 643.031 kb on - strand at 643.056 kb on - strand at 643.056 kb on - strand at 643.057 kb on + strand at 643.062 kb on + strand at 643.062 kb on + strand at 643.122 kb on + strand at 643.132 kb on + strand at 643.302 kb on - strand, within MMP_RS03420 at 643.327 kb on + strand, within MMP_RS03420 at 643.576 kb on + strand, within MMP_RS03420 at 643.615 kb on - strand at 643.616 kb on + strand at 643.655 kb on + strand at 643.765 kb on + strand at 643.903 kb on + strand at 643.972 kb on - strand at 643.987 kb on - strand at 643.988 kb on + strand at 644.071 kb on + strand at 644.090 kb on + strand at 644.090 kb on + strand at 644.129 kb on - strand at 644.145 kb on + strand at 644.147 kb on + strand at 644.262 kb on - strand, within MMP_RS03425 at 644.268 kb on - strand, within MMP_RS03425 at 644.307 kb on - strand, within MMP_RS03425 at 644.324 kb on - strand, within MMP_RS03425 at 644.324 kb on - strand, within MMP_RS03425 at 644.354 kb on + strand, within MMP_RS03425 at 644.419 kb on - strand, within MMP_RS03425 at 644.431 kb on + strand, within MMP_RS03425 at 644.485 kb on + strand, within MMP_RS03425 at 644.495 kb on + strand, within MMP_RS03425 at 644.562 kb on - strand, within MMP_RS03425 at 644.569 kb on - strand, within MMP_RS03425 at 644.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 642,510 - -0.6 642,942 - -1.7 643,031 - -0.8 643,031 - -1.7 643,031 - -1.0 643,056 - -1.2 643,056 - -1.9 643,057 + -1.3 643,062 + -1.8 643,062 + -2.1 643,122 + +0.3 643,132 + -0.1 643,302 - MMP_RS03420 0.29 +0.0 643,327 + MMP_RS03420 0.34 -0.5 643,576 + MMP_RS03420 0.83 +0.3 643,615 - -0.2 643,616 + +0.4 643,655 + -0.1 643,765 + +0.9 643,903 + +0.2 643,972 - -0.5 643,987 - -0.4 643,988 + +0.1 644,071 + -0.3 644,090 + +0.7 644,090 + -0.2 644,129 - +0.0 644,145 + +0.2 644,147 + +0.5 644,262 - MMP_RS03425 0.20 -0.2 644,268 - MMP_RS03425 0.22 -0.2 644,307 - MMP_RS03425 0.30 -0.3 644,324 - MMP_RS03425 0.34 -0.3 644,324 - MMP_RS03425 0.34 +0.0 644,354 + MMP_RS03425 0.41 -0.3 644,419 - MMP_RS03425 0.55 -0.2 644,431 + MMP_RS03425 0.58 -0.1 644,485 + MMP_RS03425 0.70 -0.5 644,495 + MMP_RS03425 0.72 +0.3 644,562 - MMP_RS03425 0.87 +0.0 644,569 - MMP_RS03425 0.88 +0.3 644,621 + -0.2
Or see this region's nucleotide sequence