Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01250 and MMP_RS01255 are separated by 33 nucleotides MMP_RS01255 and MMP_RS01260 are separated by 40 nucleotides MMP_RS01260 and MMP_RS01265 are separated by 81 nucleotides MMP_RS01265 and MMP_RS01270 are separated by 14 nucleotides
MMP_RS01250: MMP_RS01250 - tRNA (guanine(26)-N(2))-dimethyltransferase, at 236,852 to 237,973
_RS01250
MMP_RS01255: MMP_RS01255 - amino acid ABC transporter ATP-binding protein, at 238,007 to 238,738
_RS01255
MMP_RS01260: MMP_RS01260 - HDIG domain-containing protein, at 238,779 to 239,465
_RS01260
MMP_RS01265: MMP_RS01265 - hypothetical protein, at 239,547 to 239,915
_RS01265
MMP_RS01270: MMP_RS01270 - prepilin peptidase, at 239,930 to 240,970
_RS01270
Position (kb)
238
239
240 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 237.872 kb on + strand at 237.877 kb on - strand at 237.983 kb on + strand at 237.989 kb on - strand at 237.993 kb on - strand at 237.994 kb on + strand at 237.994 kb on + strand at 238.009 kb on + strand at 238.066 kb on - strand at 238.067 kb on + strand at 238.205 kb on - strand, within MMP_RS01255 at 238.215 kb on + strand, within MMP_RS01255 at 238.262 kb on - strand, within MMP_RS01255 at 238.450 kb on - strand, within MMP_RS01255 at 238.646 kb on - strand, within MMP_RS01255 at 239.228 kb on + strand, within MMP_RS01260 at 239.394 kb on - strand, within MMP_RS01260 at 239.394 kb on - strand, within MMP_RS01260 at 239.394 kb on - strand, within MMP_RS01260 at 239.397 kb on + strand at 239.454 kb on - strand at 239.454 kb on - strand at 239.482 kb on - strand at 239.482 kb on - strand at 239.483 kb on + strand at 239.485 kb on + strand at 239.486 kb on - strand at 239.499 kb on + strand at 239.512 kb on + strand at 239.544 kb on - strand at 239.544 kb on - strand at 239.546 kb on - strand at 239.546 kb on - strand at 239.546 kb on - strand at 239.547 kb on + strand at 239.804 kb on - strand, within MMP_RS01265 at 239.808 kb on + strand, within MMP_RS01265 at 239.868 kb on + strand, within MMP_RS01265 at 239.869 kb on - strand, within MMP_RS01265 at 239.999 kb on - strand at 240.000 kb on + strand at 240.001 kb on - strand at 240.001 kb on - strand at 240.052 kb on + strand, within MMP_RS01270 at 240.132 kb on - strand, within MMP_RS01270 at 240.166 kb on - strand, within MMP_RS01270 at 240.167 kb on + strand, within MMP_RS01270 at 240.175 kb on - strand, within MMP_RS01270 at 240.208 kb on - strand, within MMP_RS01270 at 240.208 kb on - strand, within MMP_RS01270 at 240.208 kb on - strand, within MMP_RS01270 at 240.209 kb on + strand, within MMP_RS01270 at 240.212 kb on - strand, within MMP_RS01270 at 240.213 kb on + strand, within MMP_RS01270 at 240.213 kb on + strand, within MMP_RS01270 at 240.249 kb on - strand, within MMP_RS01270 at 240.250 kb on + strand, within MMP_RS01270 at 240.311 kb on + strand, within MMP_RS01270 at 240.354 kb on - strand, within MMP_RS01270 at 240.400 kb on + strand, within MMP_RS01270 at 240.415 kb on + strand, within MMP_RS01270
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 237,872 + -0.9 237,877 - -1.8 237,983 + +0.4 237,989 - +0.5 237,993 - -0.7 237,994 + -0.2 237,994 + -0.3 238,009 + +0.1 238,066 - -0.4 238,067 + +0.2 238,205 - MMP_RS01255 0.27 -0.3 238,215 + MMP_RS01255 0.28 -0.0 238,262 - MMP_RS01255 0.35 -0.9 238,450 - MMP_RS01255 0.61 -0.4 238,646 - MMP_RS01255 0.87 -0.3 239,228 + MMP_RS01260 0.65 +0.3 239,394 - MMP_RS01260 0.90 +0.5 239,394 - MMP_RS01260 0.90 +0.4 239,394 - MMP_RS01260 0.90 -0.1 239,397 + -0.3 239,454 - -0.0 239,454 - +0.1 239,482 - +0.3 239,482 - +0.4 239,483 + +0.1 239,485 + +0.2 239,486 - -0.1 239,499 + +0.1 239,512 + +0.2 239,544 - -2.1 239,544 - -1.9 239,546 - -2.9 239,546 - -2.3 239,546 - -1.9 239,547 + -1.4 239,804 - MMP_RS01265 0.70 -3.2 239,808 + MMP_RS01265 0.71 -1.5 239,868 + MMP_RS01265 0.87 -2.9 239,869 - MMP_RS01265 0.87 -0.5 239,999 - -0.3 240,000 + +0.4 240,001 - -0.1 240,001 - +0.2 240,052 + MMP_RS01270 0.12 -0.2 240,132 - MMP_RS01270 0.19 +0.1 240,166 - MMP_RS01270 0.23 +0.2 240,167 + MMP_RS01270 0.23 +0.1 240,175 - MMP_RS01270 0.24 +0.1 240,208 - MMP_RS01270 0.27 -0.1 240,208 - MMP_RS01270 0.27 +0.2 240,208 - MMP_RS01270 0.27 +0.1 240,209 + MMP_RS01270 0.27 +0.0 240,212 - MMP_RS01270 0.27 +0.2 240,213 + MMP_RS01270 0.27 +0.3 240,213 + MMP_RS01270 0.27 -0.4 240,249 - MMP_RS01270 0.31 +0.2 240,250 + MMP_RS01270 0.31 +0.0 240,311 + MMP_RS01270 0.37 +0.0 240,354 - MMP_RS01270 0.41 +0.2 240,400 + MMP_RS01270 0.45 +0.1 240,415 + MMP_RS01270 0.47 +0.3
Or see this region's nucleotide sequence