Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00820 and MMP_RS00825 are separated by 27 nucleotides MMP_RS00825 and MMP_RS00830 are separated by 15 nucleotides MMP_RS00830 and MMP_RS00835 are separated by 31 nucleotides
MMP_RS00820: MMP_RS00820 - DUF2117 domain-containing protein, at 153,055 to 154,170
_RS00820
MMP_RS00825: MMP_RS00825 - fumarylacetoacetate hydrolase family protein, at 154,198 to 154,806
_RS00825
MMP_RS00830: MMP_RS00830 - purine phosphoribosyltransferase family protein, at 154,822 to 155,379
_RS00830
MMP_RS00835: MMP_RS00835 - FAD-dependent oxidoreductase, at 155,411 to 156,817
_RS00835
Position (kb) 154
155 Strain fitness (log2 ratio) -2
-1
0
1 at 153.213 kb on - strand at 153.327 kb on - strand at 153.345 kb on - strand at 153.355 kb on - strand at 153.562 kb on + strand at 153.562 kb on + strand at 153.574 kb on + strand at 153.592 kb on - strand at 154.681 kb on + strand, within MMP_RS00825 at 154.684 kb on - strand, within MMP_RS00825 at 154.684 kb on - strand, within MMP_RS00825 at 154.711 kb on - strand, within MMP_RS00825 at 154.740 kb on - strand, within MMP_RS00825 at 154.798 kb on - strand at 155.145 kb on + strand, within MMP_RS00830 at 155.309 kb on + strand, within MMP_RS00830 at 155.443 kb on + strand at 155.616 kb on - strand at 155.638 kb on - strand at 155.663 kb on + strand at 155.777 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 153,213 - -0.7 153,327 - -0.8 153,345 - -1.6 153,355 - -2.5 153,562 + -1.5 153,562 + -2.0 153,574 + -0.8 153,592 - -1.0 154,681 + MMP_RS00825 0.79 +0.3 154,684 - MMP_RS00825 0.80 +0.5 154,684 - MMP_RS00825 0.80 +0.5 154,711 - MMP_RS00825 0.84 +1.1 154,740 - MMP_RS00825 0.89 +0.5 154,798 - +0.4 155,145 + MMP_RS00830 0.58 -0.0 155,309 + MMP_RS00830 0.87 +0.1 155,443 + -0.2 155,616 - -1.1 155,638 - -2.0 155,663 + +0.3 155,777 + -0.3
Or see this region's nucleotide sequence