Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3312 and Dsui_3313 are separated by 63 nucleotides Dsui_3313 and Dsui_3314 are separated by 108 nucleotides Dsui_3314 and Dsui_3315 are separated by 10 nucleotides Dsui_3315 and Dsui_3316 are separated by 18 nucleotides Dsui_3316 and Dsui_3317 are separated by 105 nucleotides
Dsui_3312: Dsui_3312 - hypothetical protein, at 3,554,286 to 3,554,783
_3312
Dsui_3313: Dsui_3313 - hypothetical protein, at 3,554,847 to 3,555,242
_3313
Dsui_3314: Dsui_3314 - biopolymer transport protein, at 3,555,351 to 3,555,767
_3314
Dsui_3315: Dsui_3315 - biopolymer transport protein, at 3,555,778 to 3,556,497
_3315
Dsui_3316: Dsui_3316 - Gram-negative bacterial tonB protein, at 3,556,516 to 3,557,289
_3316
Dsui_3317: Dsui_3317 - cytochrome c551/c552, at 3,557,395 to 3,557,733
_3317
Position (kb)
3555
3556
3557 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3554.845 kb on + strand at 3554.853 kb on - strand at 3554.855 kb on + strand at 3554.855 kb on + strand at 3554.863 kb on - strand at 3554.869 kb on + strand at 3554.911 kb on - strand, within Dsui_3313 at 3555.015 kb on - strand, within Dsui_3313 at 3555.080 kb on + strand at 3555.105 kb on + strand, within Dsui_3313 at 3555.113 kb on - strand, within Dsui_3313 at 3555.225 kb on - strand at 3555.251 kb on + strand at 3555.260 kb on - strand at 3555.277 kb on + strand at 3555.337 kb on + strand at 3555.342 kb on + strand at 3555.561 kb on + strand, within Dsui_3314 at 3555.589 kb on - strand, within Dsui_3314 at 3555.696 kb on + strand, within Dsui_3314 at 3555.808 kb on - strand at 3555.820 kb on - strand at 3555.830 kb on + strand at 3555.908 kb on - strand, within Dsui_3315 at 3555.961 kb on + strand at 3556.002 kb on - strand, within Dsui_3315 at 3556.111 kb on + strand at 3556.119 kb on - strand, within Dsui_3315 at 3556.124 kb on - strand, within Dsui_3315 at 3556.223 kb on - strand, within Dsui_3315 at 3556.230 kb on - strand, within Dsui_3315 at 3556.323 kb on + strand, within Dsui_3315 at 3556.381 kb on - strand, within Dsui_3315 at 3556.449 kb on + strand at 3556.511 kb on - strand at 3556.513 kb on + strand at 3556.587 kb on - strand at 3556.632 kb on + strand at 3556.673 kb on + strand at 3556.762 kb on - strand, within Dsui_3316 at 3556.798 kb on - strand, within Dsui_3316 at 3556.802 kb on - strand, within Dsui_3316 at 3556.845 kb on - strand, within Dsui_3316 at 3556.964 kb on + strand, within Dsui_3316 at 3556.972 kb on - strand, within Dsui_3316 at 3557.093 kb on + strand at 3557.116 kb on - strand, within Dsui_3316 at 3557.177 kb on - strand, within Dsui_3316 at 3557.187 kb on - strand, within Dsui_3316 at 3557.239 kb on + strand at 3557.294 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 3,554,845 + +0.7 3,554,853 - -0.9 3,554,855 + -0.9 3,554,855 + +0.7 3,554,863 - -3.6 3,554,869 + -0.3 3,554,911 - Dsui_3313 0.16 -0.3 3,555,015 - Dsui_3313 0.42 -3.1 3,555,080 + +0.7 3,555,105 + Dsui_3313 0.65 +0.7 3,555,113 - Dsui_3313 0.67 -0.6 3,555,225 - +0.3 3,555,251 + -0.9 3,555,260 - -0.5 3,555,277 + -0.3 3,555,337 + -2.3 3,555,342 + -0.3 3,555,561 + Dsui_3314 0.50 -0.3 3,555,589 - Dsui_3314 0.57 +0.0 3,555,696 + Dsui_3314 0.83 -1.9 3,555,808 - +0.0 3,555,820 - -0.5 3,555,830 + -0.3 3,555,908 - Dsui_3315 0.18 -0.7 3,555,961 + -0.3 3,556,002 - Dsui_3315 0.31 -0.8 3,556,111 + +0.7 3,556,119 - Dsui_3315 0.47 -2.8 3,556,124 - Dsui_3315 0.48 -0.5 3,556,223 - Dsui_3315 0.62 -0.5 3,556,230 - Dsui_3315 0.63 -0.9 3,556,323 + Dsui_3315 0.76 -1.6 3,556,381 - Dsui_3315 0.84 -2.0 3,556,449 + +0.7 3,556,511 - +0.0 3,556,513 + -2.3 3,556,587 - -0.3 3,556,632 + -0.9 3,556,673 + -0.9 3,556,762 - Dsui_3316 0.32 -0.5 3,556,798 - Dsui_3316 0.36 -0.6 3,556,802 - Dsui_3316 0.37 -0.8 3,556,845 - Dsui_3316 0.43 -0.2 3,556,964 + Dsui_3316 0.58 -0.3 3,556,972 - Dsui_3316 0.59 -0.3 3,557,093 + +0.7 3,557,116 - Dsui_3316 0.78 -0.5 3,557,177 - Dsui_3316 0.85 -0.4 3,557,187 - Dsui_3316 0.87 -0.8 3,557,239 + -0.9 3,557,294 + -1.3
Or see this region's nucleotide sequence