Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2918 and Dsui_2919 are separated by 161 nucleotides Dsui_2919 and Dsui_2920 are separated by 47 nucleotides Dsui_2920 and Dsui_2921 overlap by 4 nucleotides
Dsui_2918: Dsui_2918 - hypothetical protein, at 3,119,605 to 3,120,285
_2918
Dsui_2919: Dsui_2919 - addiction module antidote protein, HigA family, at 3,120,447 to 3,120,761
_2919
Dsui_2920: Dsui_2920 - acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, at 3,120,809 to 3,122,239
_2920
Dsui_2921: Dsui_2921 - isochorismate synthase family protein, at 3,122,236 to 3,123,675
_2921
Position (kb)
3120
3121
3122
3123 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3119.987 kb on - strand at 3119.991 kb on + strand at 3120.110 kb on - strand at 3120.160 kb on - strand at 3120.367 kb on + strand at 3120.463 kb on + strand at 3120.465 kb on + strand at 3120.478 kb on + strand at 3120.486 kb on - strand at 3120.488 kb on + strand, within Dsui_2919 at 3120.488 kb on + strand at 3120.598 kb on + strand at 3120.598 kb on + strand, within Dsui_2919 at 3120.600 kb on + strand, within Dsui_2919 at 3120.702 kb on + strand, within Dsui_2919 at 3120.770 kb on + strand at 3120.850 kb on - strand at 3120.891 kb on + strand at 3120.891 kb on + strand at 3121.005 kb on + strand, within Dsui_2920 at 3121.024 kb on + strand, within Dsui_2920 at 3121.262 kb on - strand at 3121.267 kb on - strand at 3121.750 kb on + strand, within Dsui_2920 at 3121.842 kb on - strand at 3121.880 kb on + strand, within Dsui_2920 at 3122.074 kb on - strand at 3122.110 kb on + strand at 3122.123 kb on - strand at 3122.560 kb on + strand at 3122.593 kb on - strand at 3122.596 kb on + strand, within Dsui_2921 at 3122.615 kb on - strand at 3122.713 kb on + strand, within Dsui_2921 at 3122.716 kb on - strand, within Dsui_2921 at 3122.804 kb on - strand at 3122.908 kb on + strand at 3123.106 kb on + strand, within Dsui_2921 at 3123.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 3,119,987 - +0.8 3,119,991 + +0.8 3,120,110 - -0.2 3,120,160 - +0.8 3,120,367 + -0.2 3,120,463 + -2.0 3,120,465 + +0.8 3,120,478 + -0.2 3,120,486 - +0.8 3,120,488 + Dsui_2919 0.13 -0.2 3,120,488 + +0.8 3,120,598 + +0.8 3,120,598 + Dsui_2919 0.48 -0.2 3,120,600 + Dsui_2919 0.49 -0.2 3,120,702 + Dsui_2919 0.81 -2.0 3,120,770 + -2.0 3,120,850 - -0.2 3,120,891 + +0.4 3,120,891 + -1.7 3,121,005 + Dsui_2920 0.14 -2.2 3,121,024 + Dsui_2920 0.15 -2.0 3,121,262 - -0.2 3,121,267 - +0.8 3,121,750 + Dsui_2920 0.66 +0.8 3,121,842 - +0.8 3,121,880 + Dsui_2920 0.75 -1.8 3,122,074 - +0.8 3,122,110 + -0.8 3,122,123 - -0.8 3,122,560 + +0.2 3,122,593 - +0.8 3,122,596 + Dsui_2921 0.25 -0.4 3,122,615 - -0.2 3,122,713 + Dsui_2921 0.33 -1.4 3,122,716 - Dsui_2921 0.33 -1.2 3,122,804 - -0.2 3,122,908 + +2.8 3,123,106 + Dsui_2921 0.60 -0.5 3,123,109 - +0.8
Or see this region's nucleotide sequence