Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2814 and Dsui_2815 are separated by 256 nucleotides Dsui_2815 and Dsui_2816 are separated by 110 nucleotides Dsui_2816 and Dsui_2817 are separated by 184 nucleotides
Dsui_2814: Dsui_2814 - polyphosphate kinase 1, at 2,988,455 to 2,990,563
_2814
Dsui_2815: Dsui_2815 - putative flavoprotein, at 2,990,820 to 2,991,386
_2815
Dsui_2816: Dsui_2816 - (p)ppGpp synthetase, RelA/SpoT family, at 2,991,497 to 2,993,719
_2816
Dsui_2817: Dsui_2817 - DMT(drug/metabolite transporter) superfamily permease, at 2,993,904 to 2,994,830
_2817
Position (kb)
2991
2992
2993
2994 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2990.512 kb on + strand at 2990.697 kb on + strand at 2990.747 kb on + strand at 2990.747 kb on + strand at 2990.755 kb on - strand at 2990.755 kb on - strand at 2990.798 kb on - strand at 2990.853 kb on - strand at 2990.871 kb on - strand at 2990.916 kb on + strand, within Dsui_2815 at 2991.015 kb on + strand, within Dsui_2815 at 2991.118 kb on + strand, within Dsui_2815 at 2991.219 kb on + strand, within Dsui_2815 at 2991.227 kb on - strand, within Dsui_2815 at 2991.262 kb on - strand, within Dsui_2815 at 2991.296 kb on + strand, within Dsui_2815 at 2991.363 kb on + strand at 2991.388 kb on - strand at 2991.468 kb on - strand at 2991.570 kb on - strand at 2991.804 kb on - strand, within Dsui_2816 at 2991.836 kb on - strand, within Dsui_2816 at 2992.278 kb on - strand, within Dsui_2816 at 2992.349 kb on - strand, within Dsui_2816 at 2992.458 kb on - strand, within Dsui_2816 at 2992.524 kb on - strand, within Dsui_2816 at 2992.701 kb on - strand, within Dsui_2816 at 2992.825 kb on - strand, within Dsui_2816 at 2992.882 kb on - strand, within Dsui_2816 at 2992.882 kb on - strand, within Dsui_2816 at 2993.021 kb on - strand, within Dsui_2816 at 2993.573 kb on - strand at 2993.687 kb on - strand at 2993.702 kb on - strand at 2993.876 kb on + strand at 2993.876 kb on + strand at 2993.901 kb on + strand at 2993.929 kb on - strand at 2993.982 kb on + strand at 2994.072 kb on + strand, within Dsui_2817 at 2994.180 kb on + strand, within Dsui_2817 at 2994.180 kb on + strand, within Dsui_2817 at 2994.183 kb on + strand, within Dsui_2817 at 2994.185 kb on + strand, within Dsui_2817 at 2994.193 kb on - strand, within Dsui_2817 at 2994.315 kb on - strand at 2994.358 kb on + strand, within Dsui_2817 at 2994.413 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 2,990,512 + -1.7 2,990,697 + +0.8 2,990,747 + +0.8 2,990,747 + -0.2 2,990,755 - +0.8 2,990,755 - -0.2 2,990,798 - +0.8 2,990,853 - -1.8 2,990,871 - -0.8 2,990,916 + Dsui_2815 0.17 -1.2 2,991,015 + Dsui_2815 0.34 -1.8 2,991,118 + Dsui_2815 0.53 -3.0 2,991,219 + Dsui_2815 0.70 -2.2 2,991,227 - Dsui_2815 0.72 +0.8 2,991,262 - Dsui_2815 0.78 -0.2 2,991,296 + Dsui_2815 0.84 +0.8 2,991,363 + -1.2 2,991,388 - -1.8 2,991,468 - -0.3 2,991,570 - -4.2 2,991,804 - Dsui_2816 0.14 -2.0 2,991,836 - Dsui_2816 0.15 -4.2 2,992,278 - Dsui_2816 0.35 -3.9 2,992,349 - Dsui_2816 0.38 -4.0 2,992,458 - Dsui_2816 0.43 -5.4 2,992,524 - Dsui_2816 0.46 -6.4 2,992,701 - Dsui_2816 0.54 -2.6 2,992,825 - Dsui_2816 0.60 -1.8 2,992,882 - Dsui_2816 0.62 -1.5 2,992,882 - Dsui_2816 0.62 -3.6 2,993,021 - Dsui_2816 0.69 -2.5 2,993,573 - -3.3 2,993,687 - -2.8 2,993,702 - -1.8 2,993,876 + +0.8 2,993,876 + +0.8 2,993,901 + -0.2 2,993,929 - -0.2 2,993,982 + -2.0 2,994,072 + Dsui_2817 0.18 +0.8 2,994,180 + Dsui_2817 0.30 +1.4 2,994,180 + Dsui_2817 0.30 +0.8 2,994,183 + Dsui_2817 0.30 -1.8 2,994,185 + Dsui_2817 0.30 -1.8 2,994,193 - Dsui_2817 0.31 +0.8 2,994,315 - -0.2 2,994,358 + Dsui_2817 0.49 -0.8 2,994,413 - +0.8
Or see this region's nucleotide sequence