Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2480 and Dsui_2481 overlap by 4 nucleotides Dsui_2481 and Dsui_2482 are separated by 71 nucleotides Dsui_2482 and Dsui_2483 overlap by 11 nucleotides
Dsui_2480: Dsui_2480 - nitrous oxidase accessory protein, at 2,630,518 to 2,631,753
_2480
Dsui_2481: Dsui_2481 - DNA polymerase III, epsilon subunit, at 2,631,750 to 2,632,469
_2481
Dsui_2482: Dsui_2482 - ribonuclease HI, at 2,632,541 to 2,632,993
_2482
Dsui_2483: Dsui_2483 - methylase involved in ubiquinone/menaquinone biosynthesis, at 2,632,983 to 2,633,711
_2483
Position (kb)
2631
2632
2633 Strain fitness (log2 ratio)
-1
0
1 at 2630.792 kb on + strand, within Dsui_2480 at 2630.799 kb on - strand, within Dsui_2480 at 2630.884 kb on + strand at 2631.108 kb on - strand, within Dsui_2480 at 2631.293 kb on - strand, within Dsui_2480 at 2631.381 kb on + strand at 2631.415 kb on + strand, within Dsui_2480 at 2631.428 kb on - strand, within Dsui_2480 at 2631.493 kb on - strand, within Dsui_2480 at 2631.663 kb on + strand at 2631.734 kb on - strand at 2632.499 kb on - strand at 2632.524 kb on - strand at 2632.524 kb on - strand at 2632.545 kb on - strand at 2632.975 kb on - strand at 2632.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 2,630,792 + Dsui_2480 0.22 +0.2 2,630,799 - Dsui_2480 0.23 +0.3 2,630,884 + +0.8 2,631,108 - Dsui_2480 0.48 +0.8 2,631,293 - Dsui_2480 0.63 -0.1 2,631,381 + +0.8 2,631,415 + Dsui_2480 0.73 -0.2 2,631,428 - Dsui_2480 0.74 +0.1 2,631,493 - Dsui_2480 0.79 -0.2 2,631,663 + +0.8 2,631,734 - -0.4 2,632,499 - -0.5 2,632,524 - -0.9 2,632,524 - +0.9 2,632,545 - -0.2 2,632,975 - -1.3 2,632,998 - -0.3
Or see this region's nucleotide sequence