Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1918 and Dsui_1919 are separated by 210 nucleotides Dsui_1919 and Dsui_1920 overlap by 17 nucleotides Dsui_1920 and Dsui_1921 overlap by 23 nucleotides Dsui_1921 and Dsui_1922 are separated by 14 nucleotides
Dsui_1918: Dsui_1918 - TIGR03549 family protein, at 2,019,800 to 2,022,013
_1918
Dsui_1919: Dsui_1919 - Mg chelatase, cobalamin biosynthesis protein CobN, at 2,022,224 to 2,026,201
_1919
Dsui_1920: Dsui_1920 - hypothetical protein, at 2,026,185 to 2,026,526
_1920
Dsui_1921: Dsui_1921 - biopolymer transport protein, at 2,026,504 to 2,027,112
_1921
Dsui_1922: Dsui_1922 - outer membrane receptor for ferrienterochelin and colicins, at 2,027,127 to 2,029,406
_1922
Position (kb)
2022
2023
2024
2025
2026
2027 Strain fitness (log2 ratio)
-2
-1
0
1 at 2021.274 kb on - strand, within Dsui_1918 at 2021.281 kb on - strand, within Dsui_1918 at 2021.309 kb on - strand, within Dsui_1918 at 2021.316 kb on + strand, within Dsui_1918 at 2021.324 kb on - strand, within Dsui_1918 at 2021.324 kb on - strand, within Dsui_1918 at 2021.599 kb on + strand at 2021.608 kb on - strand, within Dsui_1918 at 2021.691 kb on - strand, within Dsui_1918 at 2021.843 kb on - strand at 2021.869 kb on + strand at 2021.927 kb on - strand at 2022.071 kb on + strand at 2022.158 kb on - strand at 2022.281 kb on + strand at 2022.568 kb on + strand at 2022.678 kb on + strand at 2022.704 kb on + strand, within Dsui_1919 at 2022.826 kb on + strand, within Dsui_1919 at 2022.898 kb on - strand at 2022.912 kb on + strand, within Dsui_1919 at 2023.112 kb on - strand at 2023.119 kb on - strand, within Dsui_1919 at 2023.156 kb on - strand at 2023.166 kb on - strand at 2023.203 kb on + strand, within Dsui_1919 at 2023.272 kb on + strand at 2023.390 kb on + strand at 2023.398 kb on - strand, within Dsui_1919 at 2023.407 kb on + strand, within Dsui_1919 at 2023.467 kb on - strand, within Dsui_1919 at 2023.536 kb on - strand at 2023.628 kb on - strand, within Dsui_1919 at 2023.664 kb on - strand, within Dsui_1919 at 2023.701 kb on - strand, within Dsui_1919 at 2023.702 kb on + strand, within Dsui_1919 at 2023.712 kb on - strand, within Dsui_1919 at 2023.753 kb on - strand at 2023.776 kb on - strand, within Dsui_1919 at 2024.027 kb on - strand at 2024.103 kb on - strand at 2024.278 kb on + strand, within Dsui_1919 at 2024.278 kb on + strand, within Dsui_1919 at 2024.393 kb on + strand at 2024.393 kb on + strand at 2024.439 kb on + strand at 2024.482 kb on - strand, within Dsui_1919 at 2024.551 kb on + strand, within Dsui_1919 at 2024.566 kb on + strand, within Dsui_1919 at 2024.678 kb on - strand at 2024.700 kb on + strand at 2024.721 kb on + strand, within Dsui_1919 at 2024.721 kb on + strand at 2024.721 kb on + strand, within Dsui_1919 at 2024.726 kb on + strand at 2024.729 kb on - strand, within Dsui_1919 at 2024.729 kb on - strand, within Dsui_1919 at 2024.729 kb on - strand, within Dsui_1919 at 2024.772 kb on + strand at 2024.930 kb on + strand, within Dsui_1919 at 2024.964 kb on - strand, within Dsui_1919 at 2024.982 kb on + strand at 2025.059 kb on - strand, within Dsui_1919 at 2025.329 kb on + strand at 2025.498 kb on - strand, within Dsui_1919 at 2025.598 kb on + strand, within Dsui_1919 at 2025.622 kb on + strand at 2025.657 kb on + strand at 2025.699 kb on + strand, within Dsui_1919 at 2025.800 kb on + strand, within Dsui_1919 at 2025.945 kb on - strand at 2026.054 kb on + strand at 2026.074 kb on + strand at 2026.106 kb on - strand at 2026.366 kb on + strand, within Dsui_1920 at 2026.366 kb on + strand, within Dsui_1920 at 2026.393 kb on + strand, within Dsui_1920 at 2026.503 kb on + strand at 2026.553 kb on - strand at 2026.800 kb on - strand, within Dsui_1921
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 2,021,274 - Dsui_1918 0.67 -1.2 2,021,281 - Dsui_1918 0.67 -0.1 2,021,309 - Dsui_1918 0.68 +0.1 2,021,316 + Dsui_1918 0.68 +0.1 2,021,324 - Dsui_1918 0.69 -1.1 2,021,324 - Dsui_1918 0.69 +0.1 2,021,599 + +1.1 2,021,608 - Dsui_1918 0.82 +1.4 2,021,691 - Dsui_1918 0.85 +0.1 2,021,843 - -1.9 2,021,869 + -1.5 2,021,927 - -0.5 2,022,071 + +0.1 2,022,158 - +0.1 2,022,281 + -0.5 2,022,568 + +1.1 2,022,678 + +1.1 2,022,704 + Dsui_1919 0.12 -0.5 2,022,826 + Dsui_1919 0.15 +0.1 2,022,898 - +1.1 2,022,912 + Dsui_1919 0.17 -0.9 2,023,112 - +1.1 2,023,119 - Dsui_1919 0.22 -1.5 2,023,156 - +1.1 2,023,166 - +1.1 2,023,203 + Dsui_1919 0.25 +1.1 2,023,272 + +1.1 2,023,390 + +1.1 2,023,398 - Dsui_1919 0.30 -0.9 2,023,407 + Dsui_1919 0.30 -0.5 2,023,467 - Dsui_1919 0.31 -2.2 2,023,536 - +0.1 2,023,628 - Dsui_1919 0.35 +0.1 2,023,664 - Dsui_1919 0.36 -0.5 2,023,701 - Dsui_1919 0.37 +0.1 2,023,702 + Dsui_1919 0.37 +1.1 2,023,712 - Dsui_1919 0.37 -0.5 2,023,753 - +1.1 2,023,776 - Dsui_1919 0.39 +1.1 2,024,027 - +1.1 2,024,103 - +1.1 2,024,278 + Dsui_1919 0.52 +1.1 2,024,278 + Dsui_1919 0.52 +0.1 2,024,393 + +1.1 2,024,393 + +1.1 2,024,439 + +1.1 2,024,482 - Dsui_1919 0.57 +0.1 2,024,551 + Dsui_1919 0.58 +0.1 2,024,566 + Dsui_1919 0.59 -1.2 2,024,678 - +1.1 2,024,700 + +0.1 2,024,721 + Dsui_1919 0.63 -0.9 2,024,721 + +1.1 2,024,721 + Dsui_1919 0.63 -1.7 2,024,726 + +1.1 2,024,729 - Dsui_1919 0.63 -0.9 2,024,729 - Dsui_1919 0.63 -0.5 2,024,729 - Dsui_1919 0.63 -2.2 2,024,772 + +0.1 2,024,930 + Dsui_1919 0.68 -0.5 2,024,964 - Dsui_1919 0.69 -1.9 2,024,982 + +1.1 2,025,059 - Dsui_1919 0.71 +1.1 2,025,329 + +1.1 2,025,498 - Dsui_1919 0.82 +0.1 2,025,598 + Dsui_1919 0.85 +0.1 2,025,622 + +1.1 2,025,657 + +0.1 2,025,699 + Dsui_1919 0.87 +0.1 2,025,800 + Dsui_1919 0.90 +1.1 2,025,945 - +1.1 2,026,054 + +0.1 2,026,074 + +1.1 2,026,106 - -0.5 2,026,366 + Dsui_1920 0.53 +1.1 2,026,366 + Dsui_1920 0.53 +0.1 2,026,393 + Dsui_1920 0.61 -0.9 2,026,503 + -0.9 2,026,553 - +1.1 2,026,800 - Dsui_1921 0.49 +0.1
Or see this region's nucleotide sequence