Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1443 and Dsui_1444 are separated by 233 nucleotides Dsui_1444 and Dsui_1445 are separated by 51 nucleotides Dsui_1445 and Dsui_1446 are separated by 11 nucleotides
Dsui_1443: Dsui_1443 - phenylacetate-CoA ligase, at 1,498,139 to 1,499,455
_1443
Dsui_1444: Dsui_1444 - Protein of unknown function (DUF2889), at 1,499,689 to 1,500,240
_1444
Dsui_1445: Dsui_1445 - succinyl-CoA synthetase, beta subunit, at 1,500,292 to 1,501,452
_1445
Dsui_1446: Dsui_1446 - succinyl-CoA synthetase, alpha subunit, at 1,501,464 to 1,502,360
_1446
Position (kb)
1500
1501
1502 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1499.331 kb on - strand at 1499.662 kb on - strand at 1499.828 kb on + strand, within Dsui_1444 at 1499.864 kb on + strand, within Dsui_1444 at 1499.929 kb on + strand, within Dsui_1444 at 1499.939 kb on + strand, within Dsui_1444 at 1499.939 kb on + strand, within Dsui_1444 at 1499.939 kb on + strand, within Dsui_1444 at 1499.939 kb on + strand, within Dsui_1444 at 1499.968 kb on + strand, within Dsui_1444 at 1499.968 kb on + strand, within Dsui_1444 at 1499.976 kb on - strand at 1499.976 kb on - strand, within Dsui_1444 at 1499.986 kb on + strand, within Dsui_1444 at 1499.987 kb on + strand, within Dsui_1444 at 1500.040 kb on + strand, within Dsui_1444 at 1500.040 kb on + strand, within Dsui_1444 at 1500.058 kb on - strand, within Dsui_1444 at 1500.058 kb on - strand, within Dsui_1444 at 1500.069 kb on + strand, within Dsui_1444 at 1500.102 kb on - strand at 1500.193 kb on + strand at 1500.201 kb on - strand at 1500.201 kb on - strand at 1500.243 kb on - strand at 1500.249 kb on + strand at 1500.249 kb on + strand at 1500.312 kb on + strand at 1500.362 kb on - strand at 1500.643 kb on + strand, within Dsui_1445 at 1500.643 kb on + strand, within Dsui_1445 at 1500.643 kb on + strand, within Dsui_1445 at 1500.651 kb on - strand, within Dsui_1445 at 1500.770 kb on + strand, within Dsui_1445 at 1500.923 kb on - strand, within Dsui_1445 at 1501.115 kb on + strand, within Dsui_1445 at 1501.123 kb on - strand, within Dsui_1445 at 1501.229 kb on - strand, within Dsui_1445 at 1501.412 kb on + strand at 1501.448 kb on + strand at 1501.448 kb on + strand at 1501.456 kb on - strand at 1501.493 kb on + strand at 1501.493 kb on + strand at 1501.628 kb on + strand, within Dsui_1446 at 1501.861 kb on + strand, within Dsui_1446 at 1501.869 kb on - strand, within Dsui_1446 at 1502.006 kb on + strand, within Dsui_1446 at 1502.052 kb on - strand, within Dsui_1446 at 1502.261 kb on - strand, within Dsui_1446
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 1,499,331 - -0.9 1,499,662 - -0.3 1,499,828 + Dsui_1444 0.25 -0.2 1,499,864 + Dsui_1444 0.32 -0.7 1,499,929 + Dsui_1444 0.43 +0.4 1,499,939 + Dsui_1444 0.45 -0.2 1,499,939 + Dsui_1444 0.45 -0.3 1,499,939 + Dsui_1444 0.45 +0.1 1,499,939 + Dsui_1444 0.45 +0.1 1,499,968 + Dsui_1444 0.51 -0.6 1,499,968 + Dsui_1444 0.51 +0.8 1,499,976 - +0.7 1,499,976 - Dsui_1444 0.52 +0.7 1,499,986 + Dsui_1444 0.54 +0.1 1,499,987 + Dsui_1444 0.54 -0.4 1,500,040 + Dsui_1444 0.64 -0.8 1,500,040 + Dsui_1444 0.64 +0.2 1,500,058 - Dsui_1444 0.67 -0.9 1,500,058 - Dsui_1444 0.67 -1.3 1,500,069 + Dsui_1444 0.69 -0.4 1,500,102 - +0.7 1,500,193 + -0.6 1,500,201 - +0.7 1,500,201 - -0.9 1,500,243 - -1.3 1,500,249 + -0.5 1,500,249 + -0.1 1,500,312 + -2.6 1,500,362 - -0.9 1,500,643 + Dsui_1445 0.30 -0.6 1,500,643 + Dsui_1445 0.30 -3.6 1,500,643 + Dsui_1445 0.30 -0.1 1,500,651 - Dsui_1445 0.31 +0.7 1,500,770 + Dsui_1445 0.41 -3.0 1,500,923 - Dsui_1445 0.54 -0.9 1,501,115 + Dsui_1445 0.71 -3.5 1,501,123 - Dsui_1445 0.72 +0.7 1,501,229 - Dsui_1445 0.81 -0.3 1,501,412 + -2.5 1,501,448 + -1.4 1,501,448 + -0.9 1,501,456 - -0.3 1,501,493 + +0.1 1,501,493 + -1.9 1,501,628 + Dsui_1446 0.18 -2.3 1,501,861 + Dsui_1446 0.44 +0.5 1,501,869 - Dsui_1446 0.45 -1.9 1,502,006 + Dsui_1446 0.60 -2.3 1,502,052 - Dsui_1446 0.66 +0.7 1,502,261 - Dsui_1446 0.89 +0.7
Or see this region's nucleotide sequence