Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0321 and Dsui_0322 are separated by 63 nucleotides Dsui_0322 and Dsui_0323 overlap by 8 nucleotides Dsui_0323 and Dsui_0324 are separated by 9 nucleotides Dsui_0324 and Dsui_0325 are separated by 7 nucleotides
Dsui_0321: Dsui_0321 - long-chain fatty acid transport protein, at 325,119 to 326,417
_0321
Dsui_0322: Dsui_0322 - acyl-CoA hydrolase, at 326,481 to 326,879
_0322
Dsui_0323: Dsui_0323 - 3-hydroxyacyl-CoA dehydrogenase, at 326,872 to 328,809
_0323
Dsui_0324: Dsui_0324 - EDD domain protein, DegV family, at 328,819 to 329,763
_0324
Dsui_0325: Dsui_0325 - acetyl-CoA acetyltransferase, at 329,771 to 331,069
_0325
Position (kb)
326
327
328
329 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 325.898 kb on - strand, within Dsui_0321 at 325.917 kb on - strand, within Dsui_0321 at 325.950 kb on - strand, within Dsui_0321 at 326.004 kb on + strand, within Dsui_0321 at 326.009 kb on + strand, within Dsui_0321 at 326.012 kb on - strand, within Dsui_0321 at 326.034 kb on + strand, within Dsui_0321 at 326.187 kb on - strand, within Dsui_0321 at 326.194 kb on + strand, within Dsui_0321 at 326.199 kb on + strand, within Dsui_0321 at 326.261 kb on + strand, within Dsui_0321 at 326.303 kb on - strand at 326.359 kb on + strand at 326.397 kb on + strand at 326.405 kb on - strand at 326.460 kb on - strand at 326.597 kb on + strand, within Dsui_0322 at 326.603 kb on - strand, within Dsui_0322 at 326.605 kb on - strand, within Dsui_0322 at 326.605 kb on - strand, within Dsui_0322 at 326.611 kb on - strand, within Dsui_0322 at 326.769 kb on + strand, within Dsui_0322 at 326.839 kb on - strand, within Dsui_0322 at 327.040 kb on + strand at 327.064 kb on + strand at 327.167 kb on - strand, within Dsui_0323 at 327.228 kb on + strand, within Dsui_0323 at 327.256 kb on + strand, within Dsui_0323 at 327.292 kb on + strand, within Dsui_0323 at 327.341 kb on + strand, within Dsui_0323 at 327.412 kb on + strand at 327.414 kb on - strand, within Dsui_0323 at 327.420 kb on - strand, within Dsui_0323 at 327.449 kb on + strand, within Dsui_0323 at 327.528 kb on + strand, within Dsui_0323 at 327.552 kb on + strand, within Dsui_0323 at 327.758 kb on + strand, within Dsui_0323 at 327.777 kb on - strand, within Dsui_0323 at 327.987 kb on - strand, within Dsui_0323 at 328.171 kb on - strand, within Dsui_0323 at 328.370 kb on + strand, within Dsui_0323 at 328.388 kb on - strand, within Dsui_0323 at 328.719 kb on - strand at 329.020 kb on - strand, within Dsui_0324 at 329.024 kb on - strand, within Dsui_0324 at 329.070 kb on - strand, within Dsui_0324 at 329.081 kb on - strand, within Dsui_0324 at 329.157 kb on + strand, within Dsui_0324 at 329.157 kb on + strand, within Dsui_0324 at 329.165 kb on - strand, within Dsui_0324 at 329.213 kb on + strand, within Dsui_0324 at 329.279 kb on - strand, within Dsui_0324 at 329.346 kb on - strand, within Dsui_0324 at 329.348 kb on + strand, within Dsui_0324 at 329.387 kb on - strand, within Dsui_0324 at 329.553 kb on + strand at 329.553 kb on + strand, within Dsui_0324 at 329.598 kb on + strand, within Dsui_0324 at 329.607 kb on - strand, within Dsui_0324
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 325,898 - Dsui_0321 0.60 +0.8 325,917 - Dsui_0321 0.61 +0.3 325,950 - Dsui_0321 0.64 -0.6 326,004 + Dsui_0321 0.68 -3.0 326,009 + Dsui_0321 0.69 +0.5 326,012 - Dsui_0321 0.69 -1.3 326,034 + Dsui_0321 0.70 -1.1 326,187 - Dsui_0321 0.82 -0.2 326,194 + Dsui_0321 0.83 +0.5 326,199 + Dsui_0321 0.83 -1.1 326,261 + Dsui_0321 0.88 +0.5 326,303 - -0.3 326,359 + +0.5 326,397 + +0.5 326,405 - +0.0 326,460 - -0.2 326,597 + Dsui_0322 0.29 +0.5 326,603 - Dsui_0322 0.31 -0.2 326,605 - Dsui_0322 0.31 -0.8 326,605 - Dsui_0322 0.31 -0.3 326,611 - Dsui_0322 0.33 +0.6 326,769 + Dsui_0322 0.72 +0.5 326,839 - Dsui_0322 0.90 -0.6 327,040 + +0.5 327,064 + -0.5 327,167 - Dsui_0323 0.15 -0.7 327,228 + Dsui_0323 0.18 -0.5 327,256 + Dsui_0323 0.20 +0.5 327,292 + Dsui_0323 0.22 -1.1 327,341 + Dsui_0323 0.24 +0.5 327,412 + -0.5 327,414 - Dsui_0323 0.28 +0.4 327,420 - Dsui_0323 0.28 -0.4 327,449 + Dsui_0323 0.30 -0.5 327,528 + Dsui_0323 0.34 -0.5 327,552 + Dsui_0323 0.35 +0.5 327,758 + Dsui_0323 0.46 -1.1 327,777 - Dsui_0323 0.47 +0.3 327,987 - Dsui_0323 0.58 +0.2 328,171 - Dsui_0323 0.67 -1.2 328,370 + Dsui_0323 0.77 -1.1 328,388 - Dsui_0323 0.78 -0.4 328,719 - -0.3 329,020 - Dsui_0324 0.21 +0.9 329,024 - Dsui_0324 0.22 -0.2 329,070 - Dsui_0324 0.27 +0.5 329,081 - Dsui_0324 0.28 -0.9 329,157 + Dsui_0324 0.36 -1.5 329,157 + Dsui_0324 0.36 -0.3 329,165 - Dsui_0324 0.37 -0.2 329,213 + Dsui_0324 0.42 -1.1 329,279 - Dsui_0324 0.49 -0.8 329,346 - Dsui_0324 0.56 +0.0 329,348 + Dsui_0324 0.56 -4.0 329,387 - Dsui_0324 0.60 +0.0 329,553 + +0.5 329,553 + Dsui_0324 0.78 -0.5 329,598 + Dsui_0324 0.82 +0.5 329,607 - Dsui_0324 0.83 +0.1
Or see this region's nucleotide sequence