Experiment: ALP with Cisplatin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0298 and Dsui_0299 are separated by 84 nucleotides Dsui_0299 and Dsui_0300 are separated by 4 nucleotides Dsui_0300 and Dsui_0301 are separated by 31 nucleotides Dsui_0301 and Dsui_0302 are separated by 160 nucleotides
Dsui_0298: Dsui_0298 - Kef-type K+ ransport system, predicted NAD-binding component, at 299,543 to 300,421
_0298
Dsui_0299: Dsui_0299 - acetyltransferase, at 300,506 to 300,991
_0299
Dsui_0300: Dsui_0300 - response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain, at 300,996 to 302,621
_0300
Dsui_0301: Dsui_0301 - histidine kinase, at 302,653 to 303,342
_0301
Dsui_0302: Dsui_0302 - UbiD family decarboxylase, at 303,503 to 304,978
_0302
Position (kb)
300
301
302
303 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 299.999 kb on - strand, within Dsui_0298 at 300.001 kb on + strand, within Dsui_0298 at 300.001 kb on + strand, within Dsui_0298 at 300.027 kb on - strand, within Dsui_0298 at 300.098 kb on - strand, within Dsui_0298 at 300.149 kb on + strand, within Dsui_0298 at 300.149 kb on + strand at 300.152 kb on - strand, within Dsui_0298 at 300.174 kb on + strand at 300.182 kb on - strand, within Dsui_0298 at 300.237 kb on - strand, within Dsui_0298 at 300.243 kb on - strand, within Dsui_0298 at 300.272 kb on + strand, within Dsui_0298 at 300.525 kb on + strand at 300.533 kb on - strand at 300.564 kb on - strand, within Dsui_0299 at 300.616 kb on + strand at 300.616 kb on + strand at 300.663 kb on + strand at 300.671 kb on - strand, within Dsui_0299 at 300.671 kb on - strand, within Dsui_0299 at 300.677 kb on + strand at 300.734 kb on + strand, within Dsui_0299 at 300.800 kb on - strand, within Dsui_0299 at 300.812 kb on - strand, within Dsui_0299 at 300.933 kb on - strand, within Dsui_0299 at 300.961 kb on + strand at 301.046 kb on - strand at 301.124 kb on + strand at 301.132 kb on - strand at 301.132 kb on - strand at 301.241 kb on + strand, within Dsui_0300 at 301.285 kb on + strand, within Dsui_0300 at 301.303 kb on - strand, within Dsui_0300 at 301.353 kb on - strand, within Dsui_0300 at 301.374 kb on + strand, within Dsui_0300 at 301.387 kb on + strand, within Dsui_0300 at 301.548 kb on + strand, within Dsui_0300 at 301.576 kb on + strand, within Dsui_0300 at 301.582 kb on + strand, within Dsui_0300 at 301.590 kb on - strand, within Dsui_0300 at 301.590 kb on - strand, within Dsui_0300 at 301.597 kb on + strand, within Dsui_0300 at 301.616 kb on + strand, within Dsui_0300 at 301.698 kb on + strand, within Dsui_0300 at 301.706 kb on - strand, within Dsui_0300 at 301.787 kb on - strand, within Dsui_0300 at 301.896 kb on + strand, within Dsui_0300 at 301.903 kb on + strand, within Dsui_0300 at 301.923 kb on - strand, within Dsui_0300 at 301.935 kb on + strand, within Dsui_0300 at 301.956 kb on - strand, within Dsui_0300 at 301.987 kb on - strand, within Dsui_0300 at 302.002 kb on - strand, within Dsui_0300 at 302.079 kb on + strand, within Dsui_0300 at 302.124 kb on + strand, within Dsui_0300 at 302.127 kb on - strand, within Dsui_0300 at 302.169 kb on + strand, within Dsui_0300 at 302.221 kb on - strand, within Dsui_0300 at 302.271 kb on - strand, within Dsui_0300 at 302.271 kb on - strand, within Dsui_0300 at 302.297 kb on - strand, within Dsui_0300 at 302.307 kb on - strand, within Dsui_0300 at 302.512 kb on - strand at 302.516 kb on + strand at 302.524 kb on - strand at 302.538 kb on + strand at 302.649 kb on - strand at 302.651 kb on + strand at 302.662 kb on + strand at 302.882 kb on - strand, within Dsui_0301 at 302.924 kb on + strand at 302.987 kb on + strand, within Dsui_0301 at 303.007 kb on + strand, within Dsui_0301 at 303.061 kb on - strand, within Dsui_0301 at 303.315 kb on + strand at 303.323 kb on - strand at 303.433 kb on - strand at 303.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cisplatin 0.0008 mg/ml remove 299,999 - Dsui_0298 0.52 +1.6 300,001 + Dsui_0298 0.52 +0.5 300,001 + Dsui_0298 0.52 +0.5 300,027 - Dsui_0298 0.55 -3.3 300,098 - Dsui_0298 0.63 +0.1 300,149 + Dsui_0298 0.69 -2.3 300,149 + +0.5 300,152 - Dsui_0298 0.69 -1.6 300,174 + +0.5 300,182 - Dsui_0298 0.73 +0.6 300,237 - Dsui_0298 0.79 -0.4 300,243 - Dsui_0298 0.80 +0.5 300,272 + Dsui_0298 0.83 -1.1 300,525 + -1.5 300,533 - -0.9 300,564 - Dsui_0299 0.12 -0.4 300,616 + -1.1 300,616 + +0.5 300,663 + +0.5 300,671 - Dsui_0299 0.34 -0.4 300,671 - Dsui_0299 0.34 -0.6 300,677 + -0.5 300,734 + Dsui_0299 0.47 -1.5 300,800 - Dsui_0299 0.60 -0.3 300,812 - Dsui_0299 0.63 -1.0 300,933 - Dsui_0299 0.88 -0.0 300,961 + -0.5 301,046 - -0.0 301,124 + +0.5 301,132 - -0.3 301,132 - -0.4 301,241 + Dsui_0300 0.15 +0.5 301,285 + Dsui_0300 0.18 +0.5 301,303 - Dsui_0300 0.19 -0.2 301,353 - Dsui_0300 0.22 -0.1 301,374 + Dsui_0300 0.23 -1.8 301,387 + Dsui_0300 0.24 -1.5 301,548 + Dsui_0300 0.34 -1.8 301,576 + Dsui_0300 0.36 -1.8 301,582 + Dsui_0300 0.36 -0.5 301,590 - Dsui_0300 0.37 -1.0 301,590 - Dsui_0300 0.37 -0.7 301,597 + Dsui_0300 0.37 -0.5 301,616 + Dsui_0300 0.38 -1.1 301,698 + Dsui_0300 0.43 +0.5 301,706 - Dsui_0300 0.44 -0.4 301,787 - Dsui_0300 0.49 -0.1 301,896 + Dsui_0300 0.55 +0.5 301,903 + Dsui_0300 0.56 -3.1 301,923 - Dsui_0300 0.57 +0.1 301,935 + Dsui_0300 0.58 -1.5 301,956 - Dsui_0300 0.59 -0.8 301,987 - Dsui_0300 0.61 +0.4 302,002 - Dsui_0300 0.62 -0.2 302,079 + Dsui_0300 0.67 -0.5 302,124 + Dsui_0300 0.69 -1.1 302,127 - Dsui_0300 0.70 -0.3 302,169 + Dsui_0300 0.72 +0.5 302,221 - Dsui_0300 0.75 -0.0 302,271 - Dsui_0300 0.78 -0.2 302,271 - Dsui_0300 0.78 +0.2 302,297 - Dsui_0300 0.80 -0.3 302,307 - Dsui_0300 0.81 -0.0 302,512 - -0.6 302,516 + +0.5 302,524 - -0.4 302,538 + +0.5 302,649 - -0.3 302,651 + -1.1 302,662 + -1.5 302,882 - Dsui_0301 0.33 -0.8 302,924 + +0.5 302,987 + Dsui_0301 0.48 -0.5 303,007 + Dsui_0301 0.51 -1.5 303,061 - Dsui_0301 0.59 -1.1 303,315 + -0.5 303,323 - -0.3 303,433 - -0.5 303,516 - -0.1
Or see this region's nucleotide sequence